DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression
Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of tes...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2023/1477658 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559696156819456 |
|---|---|
| author | Rui Hua Qingjun Chu Feiyan Guo Qinjie Chen Maocai Li Xuan Zhou Yongtong Zhu |
| author_facet | Rui Hua Qingjun Chu Feiyan Guo Qinjie Chen Maocai Li Xuan Zhou Yongtong Zhu |
| author_sort | Rui Hua |
| collection | DOAJ |
| description | Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real-time polymerase chain reaction. Mouse-derived GC-1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. The target microRNAs (miRNAs) of lncRNAs and the target mRNAs of miRNAs were predicted by a bioinformatics analysis. Functional studies performed using the CCK-8 assay, EdU incorporation assay, apoptosis detection, and senescence-associated β-galactosidase (SA-β-Gal) staining were conducted using GC-1 spg cells. Results. Totals of 2,652 lncRNAs and 2,625 mRNAs were found to be differentially expressed in the testicular tissue of NOA patients when compared with patients in a control group. Dynamin 3 opposite strand (DNM3OS) was a provider of pe-miR-214-5p that positively regulates miR-214-5p expression in GC-1 spg cells. The E2 factor (E2F) family of transcription factor 2 (E2F2) was initially predicted and subsequently verified to be a downstream gene of miR-214-5p. E2F2 expression was upregulated after DNM3OS knockdown in ADR-treated GC-1 spg cells. Moreover, knockdown of either DNM3OS or miR-214-5p significantly alleviated ADR-induced decreases in cellular activity and proliferation, as well as increases in apoptosis and senescence of mouse spermatogonial GC-1 spg cells. Conclusions. DNM3OS was found to regulate the apoptosis and senescence of spermatogonia by providing miR-214-5p and decreasing E2F2 expression, suggesting it as a novel target for gene therapy of male infertility. |
| format | Article |
| id | doaj-art-67366a1118ad4b0f8086c9839646ef48 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-67366a1118ad4b0f8086c9839646ef482025-02-03T01:29:30ZengWileyAnalytical Cellular Pathology2210-71852023-01-01202310.1155/2023/1477658DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 ExpressionRui Hua0Qingjun Chu1Feiyan Guo2Qinjie Chen3Maocai Li4Xuan Zhou5Yongtong Zhu6Center for Reproductive MedicineCenter for Reproductive MedicineCenter for Reproductive MedicineCenter for Reproductive MedicineCenter for Reproductive MedicineCenter for Reproductive MedicineCenter for Reproductive MedicineBackground. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real-time polymerase chain reaction. Mouse-derived GC-1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. The target microRNAs (miRNAs) of lncRNAs and the target mRNAs of miRNAs were predicted by a bioinformatics analysis. Functional studies performed using the CCK-8 assay, EdU incorporation assay, apoptosis detection, and senescence-associated β-galactosidase (SA-β-Gal) staining were conducted using GC-1 spg cells. Results. Totals of 2,652 lncRNAs and 2,625 mRNAs were found to be differentially expressed in the testicular tissue of NOA patients when compared with patients in a control group. Dynamin 3 opposite strand (DNM3OS) was a provider of pe-miR-214-5p that positively regulates miR-214-5p expression in GC-1 spg cells. The E2 factor (E2F) family of transcription factor 2 (E2F2) was initially predicted and subsequently verified to be a downstream gene of miR-214-5p. E2F2 expression was upregulated after DNM3OS knockdown in ADR-treated GC-1 spg cells. Moreover, knockdown of either DNM3OS or miR-214-5p significantly alleviated ADR-induced decreases in cellular activity and proliferation, as well as increases in apoptosis and senescence of mouse spermatogonial GC-1 spg cells. Conclusions. DNM3OS was found to regulate the apoptosis and senescence of spermatogonia by providing miR-214-5p and decreasing E2F2 expression, suggesting it as a novel target for gene therapy of male infertility.http://dx.doi.org/10.1155/2023/1477658 |
| spellingShingle | Rui Hua Qingjun Chu Feiyan Guo Qinjie Chen Maocai Li Xuan Zhou Yongtong Zhu DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression Analytical Cellular Pathology |
| title | DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression |
| title_full | DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression |
| title_fullStr | DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression |
| title_full_unstemmed | DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression |
| title_short | DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression |
| title_sort | dnm3os enhances the apoptosis and senescence of spermatogonia associated with nonobstructive azoospermia by providing mir 214 5p and decreasing e2f2 expression |
| url | http://dx.doi.org/10.1155/2023/1477658 |
| work_keys_str_mv | AT ruihua dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT qingjunchu dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT feiyanguo dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT qinjiechen dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT maocaili dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT xuanzhou dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression AT yongtongzhu dnm3osenhancestheapoptosisandsenescenceofspermatogoniaassociatedwithnonobstructiveazoospermiabyprovidingmir2145panddecreasinge2f2expression |