Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations
Objectives. Tumor necrosis factor alpha (TNFα) plays a pivotal role in the inflammatory host response. The serum-level of TNFα and the production of TNFα by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducib...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/972609 |
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| author | T. Mózes I. Baráth K. Gornicsar A. Grosz Ta. Mózes Cs. Göndöcs P. Széphalmi K. Gaál E. Madarász |
| author_facet | T. Mózes I. Baráth K. Gornicsar A. Grosz Ta. Mózes Cs. Göndöcs P. Széphalmi K. Gaál E. Madarász |
| author_sort | T. Mózes |
| collection | DOAJ |
| description | Objectives. Tumor necrosis factor alpha (TNFα) plays a pivotal role in the inflammatory host response. The serum-level of TNFα and the production of TNFα by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNFα-activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNFα levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNFα levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50% of volunteers with low serum-TNFα activity, LPS stimulation failed to increase the TNFα production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications. |
| format | Article |
| id | doaj-art-672ed37b0a56496fa002db4bdec433c7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-672ed37b0a56496fa002db4bdec433c72025-02-03T05:47:07ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/972609972609Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human PopulationsT. Mózes0I. Baráth1K. Gornicsar2A. Grosz3Ta. Mózes4Cs. Göndöcs5P. Széphalmi6K. Gaál7E. Madarász8Health Centre, Ministry of Defense, Róbert Károly krt44, 1134 Budapest, HungaryDepartment of Trauma, Péterfy Hospital, Péterfy Sándor utca 14, 1076 Budapest, HungaryDepartment of Urology, Péterfy Hospital, Péterfy Sándor utca 14, 1076 Budapest, HungaryHealth Centre, Ministry of Defense, Róbert Károly krt44, 1134 Budapest, HungaryDepartment of Psychology, Eötvös Lorand University, Izabella utca 46, 1064 Budapest, HungaryDepartment of Rehabilitation, Péterfy Hospital, Péterfy Sándor utca 14, 1076 Budapest, HungaryDepartment of Trauma, Dorottya Kanizsai Hospital, Szekeres József utca 2-8, 8800 Nagykanizsa, HungaryInstitute of Experimental Medicine of Hungarian Academy of Sciences, Szigony utca 49, 1083 Budapest, HungaryInstitute of Experimental Medicine of Hungarian Academy of Sciences, Szigony utca 49, 1083 Budapest, HungaryObjectives. Tumor necrosis factor alpha (TNFα) plays a pivotal role in the inflammatory host response. The serum-level of TNFα and the production of TNFα by lympho/monocytes, however, seem to show high individual variations. The goal of the present study was to investigate the variations and inducibility of TNFα-activity in two age-groups of healthy volunteers. Methods. Sixty elderly, healthy volunteers were studied. These persons were free of malignant diseases, and within three months, they did not have any trauma or inflammatory disease and were not taking any steroids or nonsteroid anti-inflammatory drugs. Thirty young volunteers were also included. Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNFα levels were determined by bioassay using WEHI 164 cells. Results. The results indicated significant individual variations in TNFα levels of healthy volunteers irrespective of age. Subgroups with low, middle, and high serum TNF-levels were distinguished. In about 50% of volunteers with low serum-TNFα activity, LPS stimulation failed to increase the TNFα production by isolated lympho/monocytes. Conclusion. Our data suggest a chance to select individuals with enhanced sensitivity for septic complications.http://dx.doi.org/10.1155/2011/972609 |
| spellingShingle | T. Mózes I. Baráth K. Gornicsar A. Grosz Ta. Mózes Cs. Göndöcs P. Széphalmi K. Gaál E. Madarász Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations Mediators of Inflammation |
| title | Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations |
| title_full | Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations |
| title_fullStr | Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations |
| title_full_unstemmed | Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations |
| title_short | Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations |
| title_sort | deviations in circulating tnfα levels and tnfα production by mononuclear cells in healthy human populations |
| url | http://dx.doi.org/10.1155/2011/972609 |
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