Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates
Abstract Microtiter-plate-based systems are unified platforms of high-throughput experimentation (HTE). These polymeric devices are used worldwide on a daily basis—mainly in the pharmaceutical industry—for parallel syntheses, reaction optimization, various preclinical studies and high-throughput scr...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-85040-5 |
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| author | Bálint Árpád Ádám Sára Spátay Bálint Jávor Szabolcs László Levente Illés Péter Fürjes Tünde Tóth Péter Huszthy Ádám Golcs |
| author_facet | Bálint Árpád Ádám Sára Spátay Bálint Jávor Szabolcs László Levente Illés Péter Fürjes Tünde Tóth Péter Huszthy Ádám Golcs |
| author_sort | Bálint Árpád Ádám |
| collection | DOAJ |
| description | Abstract Microtiter-plate-based systems are unified platforms of high-throughput experimentation (HTE). These polymeric devices are used worldwide on a daily basis—mainly in the pharmaceutical industry—for parallel syntheses, reaction optimization, various preclinical studies and high-throughput screening methods. Accordingly, laboratory automation today aims to handle these commercially available multiwell plates, making developments focused on their modifications a priority area of modern applied research. We performed the covalent functionalization of the porous PVDF-membrane of microtiter filter plates as the essence of conventional and common sandwich plate systems by introducing a generalizable method. After surface-activation of the indifferent membrane polymer, customizable functionalization becomes feasible by covalently attached monofunctional molecular linkers. The study was designed with future adaptability, and thus, industrially widespread atmospheric plasma and two different chemical treatments were investigated and compared in terms of practical implementation, polarization effects, extent of labeling, effects on morphology and porosity as well as on permeability. For critical comparison, contact angle measurements, surface ATR-FTIR, 1H-NMR, 19F-NMR, UV–Vis spectroscopy, scanning electron microscopy and permeability tests were used. |
| format | Article |
| id | doaj-art-6716fca54fb44cb6be1c1008418fbfda |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-6716fca54fb44cb6be1c1008418fbfda2025-01-26T12:28:46ZengNature PortfolioScientific Reports2045-23222025-01-0115112010.1038/s41598-024-85040-5Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter platesBálint Árpád Ádám0Sára Spátay1Bálint Jávor2Szabolcs László3Levente Illés4Péter Fürjes5Tünde Tóth6Péter Huszthy7Ádám Golcs8Department of Organic Chemistry and Technology, Budapest University of Technology and EconomicsDepartment of Organic Chemistry and Technology, Budapest University of Technology and EconomicsDepartment of Organic Chemistry and Technology, Budapest University of Technology and EconomicsDepartment of Inorganic and Analytical Chemistry, Budapest University of Technology and EconomicsInstitute of Technical Physics and Materials Science, HUN-REN Centre for Energy ResearchInstitute of Technical Physics and Materials Science, HUN-REN Centre for Energy ResearchDepartment of Organic Chemistry and Technology, Budapest University of Technology and EconomicsDepartment of Organic Chemistry and Technology, Budapest University of Technology and EconomicsDepartment of Pharmaceutical Chemistry, Semmelweis UniversityAbstract Microtiter-plate-based systems are unified platforms of high-throughput experimentation (HTE). These polymeric devices are used worldwide on a daily basis—mainly in the pharmaceutical industry—for parallel syntheses, reaction optimization, various preclinical studies and high-throughput screening methods. Accordingly, laboratory automation today aims to handle these commercially available multiwell plates, making developments focused on their modifications a priority area of modern applied research. We performed the covalent functionalization of the porous PVDF-membrane of microtiter filter plates as the essence of conventional and common sandwich plate systems by introducing a generalizable method. After surface-activation of the indifferent membrane polymer, customizable functionalization becomes feasible by covalently attached monofunctional molecular linkers. The study was designed with future adaptability, and thus, industrially widespread atmospheric plasma and two different chemical treatments were investigated and compared in terms of practical implementation, polarization effects, extent of labeling, effects on morphology and porosity as well as on permeability. For critical comparison, contact angle measurements, surface ATR-FTIR, 1H-NMR, 19F-NMR, UV–Vis spectroscopy, scanning electron microscopy and permeability tests were used.https://doi.org/10.1038/s41598-024-85040-5PVDFMembranePlasmaSurface polarizationCovalent immobilization |
| spellingShingle | Bálint Árpád Ádám Sára Spátay Bálint Jávor Szabolcs László Levente Illés Péter Fürjes Tünde Tóth Péter Huszthy Ádám Golcs Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates Scientific Reports PVDF Membrane Plasma Surface polarization Covalent immobilization |
| title | Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates |
| title_full | Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates |
| title_fullStr | Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates |
| title_full_unstemmed | Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates |
| title_short | Atmospheric air plasma pre-activation and customizable covalent functionalization of PVDF-membranes of microtiter filter plates |
| title_sort | atmospheric air plasma pre activation and customizable covalent functionalization of pvdf membranes of microtiter filter plates |
| topic | PVDF Membrane Plasma Surface polarization Covalent immobilization |
| url | https://doi.org/10.1038/s41598-024-85040-5 |
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