Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress
Depression is a common mental disorder in modern society. A traditional Chinese medicine Huanglian-Wendan decoction with potential anti-inflammation is used as a clinical antidepressant. Our previous study showed central and peripheral inflammatory responses in a rat model of depression developed by...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/3093516 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849411302519209984 |
|---|---|
| author | Ke-Ke Jia Hong Ding Han-Wen Yu Tie-Jun Dong Ying Pan Ling-Dong Kong |
| author_facet | Ke-Ke Jia Hong Ding Han-Wen Yu Tie-Jun Dong Ying Pan Ling-Dong Kong |
| author_sort | Ke-Ke Jia |
| collection | DOAJ |
| description | Depression is a common mental disorder in modern society. A traditional Chinese medicine Huanglian-Wendan decoction with potential anti-inflammation is used as a clinical antidepressant. Our previous study showed central and peripheral inflammatory responses in a rat model of depression developed by chronic unpredictable mild stress (CUMS). Here, we investigated the anti-inflammatory activity and mechanism of Huanglian-Wendan decoction in CUMS rats. LC-MS/MS and HPLC were performed to determine the major compounds in water extract of this decoction. This study showed that Huanglian-Wendan decoction significantly increased sucrose consumption and reduced serum levels of interleukin-1 beta (IL-1β), IL-6, and alanine aminotransferase (ALT) in CUMS rats. Moreover, this decoction inhibited nuclear entry of nuclear factor-kappa B (NF-κB) with the reduction of phosphorylated protein of NF-κB (p-NF-κB) and inhibitor of NF-κB alpha (p-IκBα) and downregulated protein of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ASC), cysteinyl aspartate-specific proteinase-1 (Caspase-1), and IL-1β in liver and brain regions of CUMS rats. These findings demonstrated that Huanglian-Wendan decoction had antidepressant activity with hepatoprotection in CUMS rats coinciding with its anti-inflammation in both periphery and central. The inhibitory modulation of NF-κB and NLRP3 inflammasome activation by Huanglian-Wendan decoction may mediate its antidepressant action. |
| format | Article |
| id | doaj-art-66f9a58ff6c64cc8a08600d40ebd0a4d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-66f9a58ff6c64cc8a08600d40ebd0a4d2025-08-20T03:34:48ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/30935163093516Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild StressKe-Ke Jia0Hong Ding1Han-Wen Yu2Tie-Jun Dong3Ying Pan4Ling-Dong Kong5State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, ChinaDepression is a common mental disorder in modern society. A traditional Chinese medicine Huanglian-Wendan decoction with potential anti-inflammation is used as a clinical antidepressant. Our previous study showed central and peripheral inflammatory responses in a rat model of depression developed by chronic unpredictable mild stress (CUMS). Here, we investigated the anti-inflammatory activity and mechanism of Huanglian-Wendan decoction in CUMS rats. LC-MS/MS and HPLC were performed to determine the major compounds in water extract of this decoction. This study showed that Huanglian-Wendan decoction significantly increased sucrose consumption and reduced serum levels of interleukin-1 beta (IL-1β), IL-6, and alanine aminotransferase (ALT) in CUMS rats. Moreover, this decoction inhibited nuclear entry of nuclear factor-kappa B (NF-κB) with the reduction of phosphorylated protein of NF-κB (p-NF-κB) and inhibitor of NF-κB alpha (p-IκBα) and downregulated protein of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ASC), cysteinyl aspartate-specific proteinase-1 (Caspase-1), and IL-1β in liver and brain regions of CUMS rats. These findings demonstrated that Huanglian-Wendan decoction had antidepressant activity with hepatoprotection in CUMS rats coinciding with its anti-inflammation in both periphery and central. The inhibitory modulation of NF-κB and NLRP3 inflammasome activation by Huanglian-Wendan decoction may mediate its antidepressant action.http://dx.doi.org/10.1155/2018/3093516 |
| spellingShingle | Ke-Ke Jia Hong Ding Han-Wen Yu Tie-Jun Dong Ying Pan Ling-Dong Kong Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress Mediators of Inflammation |
| title | Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress |
| title_full | Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress |
| title_fullStr | Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress |
| title_full_unstemmed | Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress |
| title_short | Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress |
| title_sort | huanglian wendan decoction inhibits nf κb nlrp3 inflammasome activation in liver and brain of rats exposed to chronic unpredictable mild stress |
| url | http://dx.doi.org/10.1155/2018/3093516 |
| work_keys_str_mv | AT kekejia huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress AT hongding huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress AT hanwenyu huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress AT tiejundong huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress AT yingpan huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress AT lingdongkong huanglianwendandecoctioninhibitsnfkbnlrp3inflammasomeactivationinliverandbrainofratsexposedtochronicunpredictablemildstress |