Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier
The usefulness of pH-sensitive fusogenic polymer-(succinylated poly(glycidol)-(SucPG-) modified liposomes as a vaccine carrier in the induction of immune responses was evaluated. Mice were intraperitoneally immunized with ovalbumin- (OVA-) containing SucPG-modified liposomes. After immunization, sig...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/903234 |
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| author | Shinobu Watarai Tana Iwase Tomoko Tajima Eiji Yuba Kenji Kono |
| author_facet | Shinobu Watarai Tana Iwase Tomoko Tajima Eiji Yuba Kenji Kono |
| author_sort | Shinobu Watarai |
| collection | DOAJ |
| description | The usefulness of pH-sensitive fusogenic polymer-(succinylated poly(glycidol)-(SucPG-) modified liposomes as a vaccine carrier in the induction of immune responses was evaluated. Mice were intraperitoneally immunized with ovalbumin- (OVA-) containing SucPG-modified liposomes. After immunization, significant OVA-specific antibodies were detected in the serum. When sera were analyzed for isotype distribution, OVA-specific IgG1 antibody responses were noted in mice immunized with OVA-containing polymer-unmodified liposomes, whereas immunization with OVA-containing SucPG-modified liposomes resulted in the induction of OVA-specific IgG1, IgG2a, and IgG3 Ab responses. In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ-(Th1-type-) and IL-4-(Th2 type-) specific mRNA were detected. Moreover, substantial production of IFN-γ and IL-4 was demonstrated in spleen cells from OVA-containing SucPG-modified liposomes in vitro. These results suggest that the pH-sensitive fusogenic polymer-(SucPG-) modified liposomes would serve effectively as an antigen delivery vehicle for inducing Th1 and Th2 immune responses. |
| format | Article |
| id | doaj-art-66ec9c90eaf244049b273964138c5ee9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-66ec9c90eaf244049b273964138c5ee92025-02-03T01:00:51ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/903234903234Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine CarrierShinobu Watarai0Tana Iwase1Tomoko Tajima2Eiji Yuba3Kenji Kono4Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, JapanDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, JapanDivision of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka 599-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai, Osaka 599-8531, JapanThe usefulness of pH-sensitive fusogenic polymer-(succinylated poly(glycidol)-(SucPG-) modified liposomes as a vaccine carrier in the induction of immune responses was evaluated. Mice were intraperitoneally immunized with ovalbumin- (OVA-) containing SucPG-modified liposomes. After immunization, significant OVA-specific antibodies were detected in the serum. When sera were analyzed for isotype distribution, OVA-specific IgG1 antibody responses were noted in mice immunized with OVA-containing polymer-unmodified liposomes, whereas immunization with OVA-containing SucPG-modified liposomes resulted in the induction of OVA-specific IgG1, IgG2a, and IgG3 Ab responses. In spleen lymphocytes from mice immunized with OVA-containing SucPG-modified liposomes, both IFN-γ-(Th1-type-) and IL-4-(Th2 type-) specific mRNA were detected. Moreover, substantial production of IFN-γ and IL-4 was demonstrated in spleen cells from OVA-containing SucPG-modified liposomes in vitro. These results suggest that the pH-sensitive fusogenic polymer-(SucPG-) modified liposomes would serve effectively as an antigen delivery vehicle for inducing Th1 and Th2 immune responses.http://dx.doi.org/10.1155/2013/903234 |
| spellingShingle | Shinobu Watarai Tana Iwase Tomoko Tajima Eiji Yuba Kenji Kono Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier The Scientific World Journal |
| title | Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier |
| title_full | Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier |
| title_fullStr | Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier |
| title_full_unstemmed | Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier |
| title_short | Efficiency of pH-Sensitive Fusogenic Polymer-Modified Liposomes as a Vaccine Carrier |
| title_sort | efficiency of ph sensitive fusogenic polymer modified liposomes as a vaccine carrier |
| url | http://dx.doi.org/10.1155/2013/903234 |
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