Cultivable Anaerobic Microbiota of Infected Root Canals
Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/609689 |
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| author | Takuichi Sato Keiko Yamaki Naoko Ishida Kazuhiro Hashimoto Yasuhisa Takeuchi Megumi Shoji Emika Sato Junko Matsuyama Hidetoshi Shimauchi Nobuhiro Takahashi |
| author_facet | Takuichi Sato Keiko Yamaki Naoko Ishida Kazuhiro Hashimoto Yasuhisa Takeuchi Megumi Shoji Emika Sato Junko Matsuyama Hidetoshi Shimauchi Nobuhiro Takahashi |
| author_sort | Takuichi Sato |
| collection | DOAJ |
| description | Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification. Methods. Informed consent was obtained from all subjects (age ranges, 34–71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Results. The mean bacterial count (CFU) in root canals was (0.5±1.1)×106 (range 8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%). The predominant isolates were Olsenella (25.4%), Mogibacterium (17.7%), Pseudoramibacter (17.7%), Propionibacterium (11.9%) and Parvimonas (5.9%). Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes. |
| format | Article |
| id | doaj-art-66e1a394f8744d3697d0aeb476daac6f |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-66e1a394f8744d3697d0aeb476daac6f2025-02-03T06:06:10ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/609689609689Cultivable Anaerobic Microbiota of Infected Root CanalsTakuichi Sato0Keiko Yamaki1Naoko Ishida2Kazuhiro Hashimoto3Yasuhisa Takeuchi4Megumi Shoji5Emika Sato6Junko Matsuyama7Hidetoshi Shimauchi8Nobuhiro Takahashi9Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Pediatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8514, JapanDivision of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanDivision of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, JapanObjective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification. Methods. Informed consent was obtained from all subjects (age ranges, 34–71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing. Results. The mean bacterial count (CFU) in root canals was (0.5±1.1)×106 (range 8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%). The predominant isolates were Olsenella (25.4%), Mogibacterium (17.7%), Pseudoramibacter (17.7%), Propionibacterium (11.9%) and Parvimonas (5.9%). Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes.http://dx.doi.org/10.1155/2012/609689 |
| spellingShingle | Takuichi Sato Keiko Yamaki Naoko Ishida Kazuhiro Hashimoto Yasuhisa Takeuchi Megumi Shoji Emika Sato Junko Matsuyama Hidetoshi Shimauchi Nobuhiro Takahashi Cultivable Anaerobic Microbiota of Infected Root Canals International Journal of Dentistry |
| title | Cultivable Anaerobic Microbiota of Infected Root Canals |
| title_full | Cultivable Anaerobic Microbiota of Infected Root Canals |
| title_fullStr | Cultivable Anaerobic Microbiota of Infected Root Canals |
| title_full_unstemmed | Cultivable Anaerobic Microbiota of Infected Root Canals |
| title_short | Cultivable Anaerobic Microbiota of Infected Root Canals |
| title_sort | cultivable anaerobic microbiota of infected root canals |
| url | http://dx.doi.org/10.1155/2012/609689 |
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