Exploring the potential of fungal proteases in aquatic environments of the Tapajós Basin, Brazil
In this study, we focused on aquatic environments and explored the fungal biodiversity and protease production in the Tapajós River Basin in Pará, Brazil. Our objectives were to identify and characterize fungal genera with potential protease activity and evaluate their biotechnological applications....
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIMS Press
2025-03-01
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| Series: | AIMS Bioengineering |
| Subjects: | |
| Online Access: | https://www.aimspress.com/article/doi/10.3934/bioeng.2025007 |
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| Summary: | In this study, we focused on aquatic environments and explored the fungal biodiversity and protease production in the Tapajós River Basin in Pará, Brazil. Our objectives were to identify and characterize fungal genera with potential protease activity and evaluate their biotechnological applications. Variations in the enzymatic activity index, colony diameters, and halo sizes were documented. For molecular identification, genomic DNA was isolated and analyzed using both the ITS region and the 28S rRNA region, which provided additional resolution for the most promising isolate. Penicillium citrinum UFOPA-MI0019 exhibited significant protease activity, suggesting potential applications in biotechnological processes. We also included the kinetics of protease production of P. citrinum UFOPA-MI0019 under solid-state fermentation using wheat bran, indicating a peak (340 ± 30 UI/mL) in enzyme activity at 72 hours of fermentation at an optimal temperature of 30 °C. These findings highlight the ecological role and industrial potential of Amazonian fungi, contributing to sustainable waste transformation. |
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| ISSN: | 2375-1495 |