The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss
A potentially probiotic autochthonous strain of Gram-positive lactic acid bacteria, Lactiplantibacillus plantarum R2 Biocenol™ (CCM 8674), was assessed as a feed supplement in rainbow trout Oncorhynchus mykiss. Three treatment groups were compared; control, fish provided L. plantarum as a four-week...
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| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2025.2458198 |
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| author | Ivana Mikulíková Marija Radojičić Hana Banďouchová Jana Blahová Ondřej Malý Ivana Papežíková Hana Novotná Eva Poštulková Zuzana Lepková Ivona Toulová Klára Odehnalová Jan Mareš Dagmar Mudroňová Miroslava Palíková |
| author_facet | Ivana Mikulíková Marija Radojičić Hana Banďouchová Jana Blahová Ondřej Malý Ivana Papežíková Hana Novotná Eva Poštulková Zuzana Lepková Ivona Toulová Klára Odehnalová Jan Mareš Dagmar Mudroňová Miroslava Palíková |
| author_sort | Ivana Mikulíková |
| collection | DOAJ |
| description | A potentially probiotic autochthonous strain of Gram-positive lactic acid bacteria, Lactiplantibacillus plantarum R2 Biocenol™ (CCM 8674), was assessed as a feed supplement in rainbow trout Oncorhynchus mykiss. Three treatment groups were compared; control, fish provided L. plantarum as a four-week dietary pulse followed by three weeks of non-supplemented diet and fish provided the supplemented feed for seven weeks. A trend of improved growth performance (specific growth rate, feed conversion ratio) was observed in both supplemented groups. Though the effect of pulse supplementation on the intestinal microbiome community was not permanent, a statistically significant difference was recorded in the composition of the gut content bacterial community in both pulse and continuous supplemented groups. A significant reduction in the abundance of several bacterial genera was observed in the gut microbiome of fish given the seven-week supplementation. Continuous supplementation significantly increased blood haemoglobin and haematocrit levels and reduced plasmatic chloride concentration, while significantly increased concentrations of plasmatic magnesium and calcium were recorded in pulse-fed fish. No significant changes in immunological indices were revealed. Our results suggest that four weeks and seven weeks supplementation of L. plantarum show similar trends of effects on growth performance in rainbow trout. Further studies will follow to assess the effects of L. plantarum R2 Biocenol™ on the response of rainbow trout to a pathogens challenge in relation to the change in gut microbiome composition. |
| format | Article |
| id | doaj-art-66bf39bdef384c5c86178af53c0619cd |
| institution | Kabale University |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-66bf39bdef384c5c86178af53c0619cd2025-02-05T12:46:15ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2025-12-0124142443910.1080/1828051X.2025.24581982458198The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykissIvana Mikulíková0Marija Radojičić1Hana Banďouchová2Jana Blahová3Ondřej Malý4Ivana Papežíková5Hana Novotná6Eva Poštulková7Zuzana Lepková8Ivona Toulová9Klára Odehnalová10Jan Mareš11Dagmar Mudroňová12Miroslava Palíková13Ústav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav zoologie, rybářství, hydrobiologie a včelařství, Agronomická fakulta, Mendel University in BrnoÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav ochrany a welfare zvířat a veřejného veterinárního lékařství, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav zoologie, rybářství, hydrobiologie a včelařství, Agronomická fakulta, Mendel University in BrnoÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav zoologie, rybářství, hydrobiologie a včelařství, Agronomická fakulta, Mendel University in BrnoÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoOddělení experimentální fykologie a ekotoxikologie, Botanický ústav, Czech Academy of SciencesÚstav zoologie, rybářství, hydrobiologie a včelařství, Agronomická fakulta, Mendel University in BrnoKatedra mikrobiológie a imunológie, University of Veterinary Medicine and Pharmacy in KošiceÚstav ekologie a chorob zoozvířat, zvěře, ryb a včel, Fakulta veterinární hygieny a ekologie, University of Veterinary Sciences BrnoA potentially probiotic autochthonous strain of Gram-positive lactic acid bacteria, Lactiplantibacillus plantarum R2 Biocenol™ (CCM 8674), was assessed as a feed supplement in rainbow trout Oncorhynchus mykiss. Three treatment groups were compared; control, fish provided L. plantarum as a four-week dietary pulse followed by three weeks of non-supplemented diet and fish provided the supplemented feed for seven weeks. A trend of improved growth performance (specific growth rate, feed conversion ratio) was observed in both supplemented groups. Though the effect of pulse supplementation on the intestinal microbiome community was not permanent, a statistically significant difference was recorded in the composition of the gut content bacterial community in both pulse and continuous supplemented groups. A significant reduction in the abundance of several bacterial genera was observed in the gut microbiome of fish given the seven-week supplementation. Continuous supplementation significantly increased blood haemoglobin and haematocrit levels and reduced plasmatic chloride concentration, while significantly increased concentrations of plasmatic magnesium and calcium were recorded in pulse-fed fish. No significant changes in immunological indices were revealed. Our results suggest that four weeks and seven weeks supplementation of L. plantarum show similar trends of effects on growth performance in rainbow trout. Further studies will follow to assess the effects of L. plantarum R2 Biocenol™ on the response of rainbow trout to a pathogens challenge in relation to the change in gut microbiome composition.http://dx.doi.org/10.1080/1828051X.2025.2458198feed supplementprobioticsgut microbiotarainbow troutlactiplantibacillii |
| spellingShingle | Ivana Mikulíková Marija Radojičić Hana Banďouchová Jana Blahová Ondřej Malý Ivana Papežíková Hana Novotná Eva Poštulková Zuzana Lepková Ivona Toulová Klára Odehnalová Jan Mareš Dagmar Mudroňová Miroslava Palíková The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss Italian Journal of Animal Science feed supplement probiotics gut microbiota rainbow trout lactiplantibacillii |
| title | The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss |
| title_full | The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss |
| title_fullStr | The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss |
| title_full_unstemmed | The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss |
| title_short | The effect of continuous and pulse feeding Lactiplantibacillus plantarum on growth performance, gut microbiota, immunological, haematological and plasma biochemical parameters of rainbow trout Oncorhynchus mykiss |
| title_sort | effect of continuous and pulse feeding lactiplantibacillus plantarum on growth performance gut microbiota immunological haematological and plasma biochemical parameters of rainbow trout oncorhynchus mykiss |
| topic | feed supplement probiotics gut microbiota rainbow trout lactiplantibacillii |
| url | http://dx.doi.org/10.1080/1828051X.2025.2458198 |
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