Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin

To predict the anti-mutant effects of antibiotic combinations against Staphylococcus aureus, the mutant prevention concentrations (MPCs) of linezolid and daptomycin were determined at a linezolid-to-daptomycin concentration ratio that corresponds to the ratio of the 24-hour areas under the concentra...

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Main Authors: K. N. Alieva, M. V. Golikova, Y. A. Portnoy, A. A. Firsov
Format: Article
Language:Russian
Published: LLC "Publishing House OKI" 2020-05-01
Series:Антибиотики и Химиотерапия
Subjects:
Online Access:https://www.antibiotics-chemotherapy.ru/jour/article/view/174
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author K. N. Alieva
M. V. Golikova
Y. A. Portnoy
A. A. Firsov
author_facet K. N. Alieva
M. V. Golikova
Y. A. Portnoy
A. A. Firsov
author_sort K. N. Alieva
collection DOAJ
description To predict the anti-mutant effects of antibiotic combinations against Staphylococcus aureus, the mutant prevention concentrations (MPCs) of linezolid and daptomycin were determined at a linezolid-to-daptomycin concentration ratio that corresponds to the ratio of the 24-hour areas under the concentration-time curve (AUC24s) that were used in pharmacokinetic simulations in an in vitro dynamic model. The enrichment of linezolid-resistant mutants of two S.aureus strains was observed under linezolid exposure. The growth of daptomycin-resistant mutants exposed to daptomycin was less pronounced. Combination therapy prevented or restricted the development of S.aureus resistance to linezolid or daptomycin, respectively. These findings support the applicability of MPC determination to predict the anti-mutant effects of combination therapy with linezolid and daptomycin.
format Article
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institution Kabale University
issn 0235-2990
language Russian
publishDate 2020-05-01
publisher LLC "Publishing House OKI"
record_format Article
series Антибиотики и Химиотерапия
spelling doaj-art-66ac2488fbbe4f00a93cd3636d375bb72025-08-20T03:37:09ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01649-10813890Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–DaptomycinK. N. Alieva0M. V. Golikova1Y. A. Portnoy2A. A. Firsov3Gause Institute of New AntibioticsGause Institute of New AntibioticsGause Institute of New AntibioticsGause Institute of New AntibioticsTo predict the anti-mutant effects of antibiotic combinations against Staphylococcus aureus, the mutant prevention concentrations (MPCs) of linezolid and daptomycin were determined at a linezolid-to-daptomycin concentration ratio that corresponds to the ratio of the 24-hour areas under the concentration-time curve (AUC24s) that were used in pharmacokinetic simulations in an in vitro dynamic model. The enrichment of linezolid-resistant mutants of two S.aureus strains was observed under linezolid exposure. The growth of daptomycin-resistant mutants exposed to daptomycin was less pronounced. Combination therapy prevented or restricted the development of S.aureus resistance to linezolid or daptomycin, respectively. These findings support the applicability of MPC determination to predict the anti-mutant effects of combination therapy with linezolid and daptomycin.https://www.antibiotics-chemotherapy.ru/jour/article/view/174linezolid, daptomycin, combination, s.aureus resistance, in vitro dynamic model
spellingShingle K. N. Alieva
M. V. Golikova
Y. A. Portnoy
A. A. Firsov
Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
Антибиотики и Химиотерапия
linezolid, daptomycin, combination, s.aureus resistance, in vitro dynamic model
title Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
title_full Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
title_fullStr Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
title_full_unstemmed Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
title_short Antibiotic Combinations Preventing Bacterial Resistance: Studies on an in vitro Model with Staphylococcus aureus Exposed to Linezolid–Daptomycin
title_sort antibiotic combinations preventing bacterial resistance studies on an in vitro model with staphylococcus aureus exposed to linezolid daptomycin
topic linezolid, daptomycin, combination, s.aureus resistance, in vitro dynamic model
url https://www.antibiotics-chemotherapy.ru/jour/article/view/174
work_keys_str_mv AT knalieva antibioticcombinationspreventingbacterialresistancestudiesonaninvitromodelwithstaphylococcusaureusexposedtolinezoliddaptomycin
AT mvgolikova antibioticcombinationspreventingbacterialresistancestudiesonaninvitromodelwithstaphylococcusaureusexposedtolinezoliddaptomycin
AT yaportnoy antibioticcombinationspreventingbacterialresistancestudiesonaninvitromodelwithstaphylococcusaureusexposedtolinezoliddaptomycin
AT aafirsov antibioticcombinationspreventingbacterialresistancestudiesonaninvitromodelwithstaphylococcusaureusexposedtolinezoliddaptomycin