Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro
Given the dynamic growth of antibiotic resistance observed over the past few decades, new therapeutic methods for life-threatening infections are crucial. Effective treatment of infections caused by multidrug-resistant microorganisms increasingly requires the use of last resort drugs, and new antibi...
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| Format: | Article |
| Language: | English |
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Sciendo
2025-01-01
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| Series: | Postępy Mikrobiologii |
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| Online Access: | https://doi.org/10.2478/am-2024-0017 |
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| author | Kmiecikowski Paweł Z. Gabriel Aniela Depka Dagmara Bogiel Tomasz |
| author_facet | Kmiecikowski Paweł Z. Gabriel Aniela Depka Dagmara Bogiel Tomasz |
| author_sort | Kmiecikowski Paweł Z. |
| collection | DOAJ |
| description | Given the dynamic growth of antibiotic resistance observed over the past few decades, new therapeutic methods for life-threatening infections are crucial. Effective treatment of infections caused by multidrug-resistant microorganisms increasingly requires the use of last resort drugs, and new antibiotics generate resistance in less time than it takes to bring them to the therapy. Therefore it is advisable to seek solutions using currently available antibiotics. Effective therapy of infections with multidrug-resistant microorganisms may be enabled by the use of additive and synergistic interactions, resulting from the combination of different groups of antibiotics with the same or different modes of action – including those to which a particular bacterial strain is resistant. This paper describes methods for determining the synergistic and additive effects of antibiotics in vitro, including the CombiANT method under testing. The purpose of this study is to present possible methods for determining the additive and synergistic effects between antibiotics in vitro and to propose appropriate laboratory procedures for their use. This paper reviews the latest literature on methods for determining the antimicrobials interactions. Clinical studies indicate significant benefits of using the phenomenon of antibiotic addition or synergy in clinical practice, not only improving the effectiveness of therapy, but also minimizing side effects and reducing the risk of developing de novo antibiotic resistance. Despite the significant problem of increasing antibiotic resistance level, clinical practice still lacks clearly standardized methods for determining synergism. Further research is needed to determine the most beneficial standard available for a wide range of microbiology diagnostic facilities. |
| format | Article |
| id | doaj-art-66a9243044f14b8f8097e65792422599 |
| institution | Kabale University |
| issn | 2545-3149 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Postępy Mikrobiologii |
| spelling | doaj-art-66a9243044f14b8f8097e657924225992025-02-02T15:47:31ZengSciendoPostępy Mikrobiologii2545-31492025-01-0163419922210.2478/am-2024-0017Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in VitroKmiecikowski Paweł Z.0Gabriel Aniela1Depka Dagmara2Bogiel Tomasz31Microbiology Student Science Club, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094Bydgoszcz, Poland1Microbiology Student Science Club, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094Bydgoszcz, Poland2Microbiology Department Ludwik Rydygier, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094 Bydgoszcz, Poland2Microbiology Department Ludwik Rydygier, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Skłodowska-Curie Street, 85-094 Bydgoszcz, PolandGiven the dynamic growth of antibiotic resistance observed over the past few decades, new therapeutic methods for life-threatening infections are crucial. Effective treatment of infections caused by multidrug-resistant microorganisms increasingly requires the use of last resort drugs, and new antibiotics generate resistance in less time than it takes to bring them to the therapy. Therefore it is advisable to seek solutions using currently available antibiotics. Effective therapy of infections with multidrug-resistant microorganisms may be enabled by the use of additive and synergistic interactions, resulting from the combination of different groups of antibiotics with the same or different modes of action – including those to which a particular bacterial strain is resistant. This paper describes methods for determining the synergistic and additive effects of antibiotics in vitro, including the CombiANT method under testing. The purpose of this study is to present possible methods for determining the additive and synergistic effects between antibiotics in vitro and to propose appropriate laboratory procedures for their use. This paper reviews the latest literature on methods for determining the antimicrobials interactions. Clinical studies indicate significant benefits of using the phenomenon of antibiotic addition or synergy in clinical practice, not only improving the effectiveness of therapy, but also minimizing side effects and reducing the risk of developing de novo antibiotic resistance. Despite the significant problem of increasing antibiotic resistance level, clinical practice still lacks clearly standardized methods for determining synergism. Further research is needed to determine the most beneficial standard available for a wide range of microbiology diagnostic facilities.https://doi.org/10.2478/am-2024-0017antibiotic resistanceantibiotic synergismcheckerboard testtime-kill test |
| spellingShingle | Kmiecikowski Paweł Z. Gabriel Aniela Depka Dagmara Bogiel Tomasz Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro Postępy Mikrobiologii antibiotic resistance antibiotic synergism checkerboard test time-kill test |
| title | Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro |
| title_full | Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro |
| title_fullStr | Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro |
| title_full_unstemmed | Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro |
| title_short | Description of Methodology for Testing the Synergistic and Additive Effects of Antibiotics in Vitro |
| title_sort | description of methodology for testing the synergistic and additive effects of antibiotics in vitro |
| topic | antibiotic resistance antibiotic synergism checkerboard test time-kill test |
| url | https://doi.org/10.2478/am-2024-0017 |
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