Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine
<b>Objectives</b>: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. <b>Materials and Methods</b>: In this single-center study, a total of 250 subjects provided urine and/or b...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/15/3/269 |
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| Summary: | <b>Objectives</b>: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. <b>Materials and Methods</b>: In this single-center study, a total of 250 subjects provided urine and/or blood samples: 234 provided urine and blood samples, and 16 provided only blood samples. Biochip-based narcotics screening was performed on 234 urine and 16 blood samples, and all samples were analyzed using tandem liquid chromatography-mass spectrometry (LC-MS/MS). <b>Results</b>: The two most prevalent narcotics were methamphetamine and amphetamine, with both biochip-based array and LC-MS/MS. Cohen’s kappa correlation indicated a substantial agreement between LC-MS/MS and biochip-based screening for both methamphetamine (κ = 0.661, <i>p</i> <0.001) and amphetamine (κ = 0.663, <i>p</i> < 0.001). LC-MS/MS demonstrated near-perfect agreement between urine and blood samples for both methamphetamine (κ = 0.855, <i>p</i> <0.001) and amphetamine (κ = 0.881, <i>p</i> < 0.001). <b>Conclusions</b>: Biochip-based arrays are a valid method offering a faster, less material-intensive alternative to LC-MS/MS for substance detection in forensic examination. |
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| ISSN: | 2075-4418 |