Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis
Introduction. Increased apoptosis of epithelial cells and decreased apoptosis of myofibroblasts are involved in the pathogenesis of IPF. The apoptotic profile of alveolar macrophages (AMs) in IPF is unclear. Aim. To investigate whether AMs of patients with IPF exhibit a different apoptotic profile c...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2012/981730 |
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| author | Fotios Drakopanagiotakis Areti Xifteri Evaggelos Tsiambas Andreas Karameris Konstantina Tsakanika Napoleon Karagiannidis Demetrios Mermigkis Vlasis Polychronopoulos Demosthenes Bouros |
| author_facet | Fotios Drakopanagiotakis Areti Xifteri Evaggelos Tsiambas Andreas Karameris Konstantina Tsakanika Napoleon Karagiannidis Demetrios Mermigkis Vlasis Polychronopoulos Demosthenes Bouros |
| author_sort | Fotios Drakopanagiotakis |
| collection | DOAJ |
| description | Introduction. Increased apoptosis of epithelial cells and decreased apoptosis of myofibroblasts are involved in the pathogenesis of IPF. The apoptotic profile of alveolar macrophages (AMs) in IPF is unclear. Aim. To investigate whether AMs of patients with IPF exhibit a different apoptotic profile compared to normal subjects. Methods. We analyzed, by immunohistochemistry, the expression of the apoptotic markers fas, fas ligand , bcl-2, and bax in AM obtained from bronchoalveolar lavage fluid (BALF) of 20 newly diagnosed, treatment-naive IPF patients and of 16 controls.
Apoptosis of AM was evaluated by Apoptag immunohistochemistry. IPF patients received either interferon-g and corticosteroids or azathioprine and corticosteroids for six months. Results. BALF AMs undergoing apoptosis were significantly less in IPF patients. No difference was found in the expression of fas or fas ligand, bcl-2 and bax between IPF
and control group. No difference was found between the respiratory function parameters of the two treatment groups after six months. A positive correlation was found between the number of bcl-2 positive stained macrophages and DLCO after treatment. Conclusions. The decreased apoptotic rate of AM of patients with IPF is not associated with decreased expression of apoptosis mediators involved in the external or internal apoptotic pathway. |
| format | Article |
| id | doaj-art-6679f107601b49469bd24df97e506ed6 |
| institution | Kabale University |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-6679f107601b49469bd24df97e506ed62025-02-03T01:10:28ZengWileyPulmonary Medicine2090-18362090-18442012-01-01201210.1155/2012/981730981730Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary FibrosisFotios Drakopanagiotakis0Areti Xifteri1Evaggelos Tsiambas2Andreas Karameris3Konstantina Tsakanika4Napoleon Karagiannidis5Demetrios Mermigkis6Vlasis Polychronopoulos7Demosthenes Bouros83rd Respiratory Medicine Department, Sismanoglio General Hospital, 15126 Marousi, Greece3rd Respiratory Medicine Department, Sismanoglio General Hospital, 15126 Marousi, GreeceDepartment of Pathology and Computerized Image Analysis, 417 NIMTS Hospital, 11521 Athens, GreeceDepartment of Pathology and Computerized Image Analysis, 417 NIMTS Hospital, 11521 Athens, GreeceBronchoalveolar Lavage Unit, Sismanoglio General Hospital, 15126 Marousi, Greece3rd Respiratory Medicine Department, Sismanoglio General Hospital, 15126 Marousi, Greece3rd Respiratory Medicine Department, Sismanoglio General Hospital, 15126 Marousi, Greece3rd Respiratory Medicine Department, Sismanoglio General Hospital, 15126 Marousi, GreeceDepartment of Pneumonology, University Hospital of Alexandroupolis and Medical School of Democritus University of Thrace, 68100 Alexandroupolis, GreeceIntroduction. Increased apoptosis of epithelial cells and decreased apoptosis of myofibroblasts are involved in the pathogenesis of IPF. The apoptotic profile of alveolar macrophages (AMs) in IPF is unclear. Aim. To investigate whether AMs of patients with IPF exhibit a different apoptotic profile compared to normal subjects. Methods. We analyzed, by immunohistochemistry, the expression of the apoptotic markers fas, fas ligand , bcl-2, and bax in AM obtained from bronchoalveolar lavage fluid (BALF) of 20 newly diagnosed, treatment-naive IPF patients and of 16 controls. Apoptosis of AM was evaluated by Apoptag immunohistochemistry. IPF patients received either interferon-g and corticosteroids or azathioprine and corticosteroids for six months. Results. BALF AMs undergoing apoptosis were significantly less in IPF patients. No difference was found in the expression of fas or fas ligand, bcl-2 and bax between IPF and control group. No difference was found between the respiratory function parameters of the two treatment groups after six months. A positive correlation was found between the number of bcl-2 positive stained macrophages and DLCO after treatment. Conclusions. The decreased apoptotic rate of AM of patients with IPF is not associated with decreased expression of apoptosis mediators involved in the external or internal apoptotic pathway.http://dx.doi.org/10.1155/2012/981730 |
| spellingShingle | Fotios Drakopanagiotakis Areti Xifteri Evaggelos Tsiambas Andreas Karameris Konstantina Tsakanika Napoleon Karagiannidis Demetrios Mermigkis Vlasis Polychronopoulos Demosthenes Bouros Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis Pulmonary Medicine |
| title | Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis |
| title_full | Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis |
| title_fullStr | Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis |
| title_full_unstemmed | Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis |
| title_short | Decreased Apoptotic Rate of Alveolar Macrophages of Patients with Idiopathic Pulmonary Fibrosis |
| title_sort | decreased apoptotic rate of alveolar macrophages of patients with idiopathic pulmonary fibrosis |
| url | http://dx.doi.org/10.1155/2012/981730 |
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