Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer
MicroRNAs (miRNAs) have been demonstrated to play an important role in carcinogenesis. Previous studies revealed that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. In this study, we measured the plasma expression levels of three miRN...
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| Format: | Article |
| Language: | English |
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BioMed Central
2015-09-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-13-70.pdf |
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| author | Jong-Lyul Park Mirang Kim Kyu-Sang Song Seon-Young Kim Yong Sung Kim |
| author_facet | Jong-Lyul Park Mirang Kim Kyu-Sang Song Seon-Young Kim Yong Sung Kim |
| author_sort | Jong-Lyul Park |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) have been demonstrated to play an important role in carcinogenesis. Previous studies revealed that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. In this study, we measured the plasma expression levels of three miRNAs (miR-21, miR-27a, and miR-155) to investigate the usefulness of miRNAs for gastric cancer detection. We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. Therefore, we further validated the miR-27a expression level in 73 paired gastric cancer tissues and in a validation plasma cohort from 35 patients with gastric cancer and 35 healthy controls. In both the gastric cancer tissues and the validation plasma cohort, the miR-27a expression levels were significantly higher in patients with gastric cancer. Receiver-operator characteristic (ROC) analysis of the validation cohort, revealed an area under the ROC curve value of 0.70 with 75% sensitivity and 56% specificity in discriminating gastric cancer. Thus, the miR-27a expression level in plasma could be a useful biomarker for the diagnosis and/or prognosis of gastric cancer. |
| format | Article |
| id | doaj-art-6666998f2bf447bf9da84d9db1ceceb8 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-6666998f2bf447bf9da84d9db1ceceb82025-02-02T00:09:29ZengBioMed CentralGenomics & Informatics1598-866X2234-07422015-09-01133707510.5808/GI.2015.13.3.70156Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric CancerJong-Lyul Park0Mirang Kim1Kyu-Sang Song2Seon-Young Kim3Yong Sung Kim4Epigenome Research Center, Genome Institute, KRIBB, Daejeon 34141, Korea.Epigenome Research Center, Genome Institute, KRIBB, Daejeon 34141, Korea.Department of Pathology, Chungnam National University College of Medicine, Daejeon 35015, Korea.Epigenome Research Center, Genome Institute, KRIBB, Daejeon 34141, Korea.Epigenome Research Center, Genome Institute, KRIBB, Daejeon 34141, Korea.MicroRNAs (miRNAs) have been demonstrated to play an important role in carcinogenesis. Previous studies revealed that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. In this study, we measured the plasma expression levels of three miRNAs (miR-21, miR-27a, and miR-155) to investigate the usefulness of miRNAs for gastric cancer detection. We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. Therefore, we further validated the miR-27a expression level in 73 paired gastric cancer tissues and in a validation plasma cohort from 35 patients with gastric cancer and 35 healthy controls. In both the gastric cancer tissues and the validation plasma cohort, the miR-27a expression levels were significantly higher in patients with gastric cancer. Receiver-operator characteristic (ROC) analysis of the validation cohort, revealed an area under the ROC curve value of 0.70 with 75% sensitivity and 56% specificity in discriminating gastric cancer. Thus, the miR-27a expression level in plasma could be a useful biomarker for the diagnosis and/or prognosis of gastric cancer.http://genominfo.org/upload/pdf/gni-13-70.pdfmiR-27aplasmastomach neoplasms |
| spellingShingle | Jong-Lyul Park Mirang Kim Kyu-Sang Song Seon-Young Kim Yong Sung Kim Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer Genomics & Informatics miR-27a plasma stomach neoplasms |
| title | Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer |
| title_full | Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer |
| title_fullStr | Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer |
| title_full_unstemmed | Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer |
| title_short | Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer |
| title_sort | cell free mir 27a a potential diagnostic and prognostic biomarker for gastric cancer |
| topic | miR-27a plasma stomach neoplasms |
| url | http://genominfo.org/upload/pdf/gni-13-70.pdf |
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