Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
In this study, a method for the simultaneous determination of neonicotinoid insecticides and their metabolites in meat was established by the QuEChERS (quick, easy, cheap, effective, rugged, safe) pretreatment method followed by ultra-high performance liquid chromatography-tandem mass spectrometry (...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2024-12-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-031.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539822054440960 |
|---|---|
| author | LIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian |
| author_facet | LIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian |
| author_sort | LIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian |
| collection | DOAJ |
| description | In this study, a method for the simultaneous determination of neonicotinoid insecticides and their metabolites in meat was established by the QuEChERS (quick, easy, cheap, effective, rugged, safe) pretreatment method followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted with acetonitrile containing 1% acetic acid. After salting-out with 1 g of NaCl and 4 g of anhydrous MgSO4, the supernatant was cleaned-up using C18. The separation of the analytes was carried out on a XSelect HSS T3 (150 mm × 3.0 mm, 2.5 μm) column by gradient elution with a mobile phase composed of 0.1% formic acid and acetonitrile. The analytes were detected by multiple reaction monitoring (MRM) in the positive ion mode and quantified by the matrix-matched external standard method. The results showed that the calibration curves for all analytes had a good linearity with correlation coefficients (r) more than 0.995. The limit of quantitation (LOQ) was 2 μg/kg for 12 target compounds and 20 μg/kg for the two compounds. The recovery rates of the proposed method were in the range of 61.0%–116.7% with relative standard deviations (RSDs) below 14.81%. This method has the merits of simple pretreatment steps and satisfying recovery rates and enables fast and accurate detection of neonicotinoid insecticides and their metabolites in meat. |
| format | Article |
| id | doaj-art-666363679e4f4a6bad2987779894a570 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-666363679e4f4a6bad2987779894a5702025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452427328110.7506/spkx1002-6630-20240519-146Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass SpectrometryLIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian0(Xiamen Institute for Food and Drug Quality Control, Xiamen 361013, China)In this study, a method for the simultaneous determination of neonicotinoid insecticides and their metabolites in meat was established by the QuEChERS (quick, easy, cheap, effective, rugged, safe) pretreatment method followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted with acetonitrile containing 1% acetic acid. After salting-out with 1 g of NaCl and 4 g of anhydrous MgSO4, the supernatant was cleaned-up using C18. The separation of the analytes was carried out on a XSelect HSS T3 (150 mm × 3.0 mm, 2.5 μm) column by gradient elution with a mobile phase composed of 0.1% formic acid and acetonitrile. The analytes were detected by multiple reaction monitoring (MRM) in the positive ion mode and quantified by the matrix-matched external standard method. The results showed that the calibration curves for all analytes had a good linearity with correlation coefficients (r) more than 0.995. The limit of quantitation (LOQ) was 2 μg/kg for 12 target compounds and 20 μg/kg for the two compounds. The recovery rates of the proposed method were in the range of 61.0%–116.7% with relative standard deviations (RSDs) below 14.81%. This method has the merits of simple pretreatment steps and satisfying recovery rates and enables fast and accurate detection of neonicotinoid insecticides and their metabolites in meat.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-031.pdfneonicotinoid insecticides; metabolites; meat; quick, easy, cheap, effective, rugged, safe; ultra-high performance liquid chromatography-tandem mass spectrometry |
| spellingShingle | LIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry Shipin Kexue neonicotinoid insecticides; metabolites; meat; quick, easy, cheap, effective, rugged, safe; ultra-high performance liquid chromatography-tandem mass spectrometry |
| title | Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | simultaneous determination of nine neonicotinoid insecticides and five metabolites in meat by quechers coupled with ultra high performance liquid chromatography tandem mass spectrometry |
| topic | neonicotinoid insecticides; metabolites; meat; quick, easy, cheap, effective, rugged, safe; ultra-high performance liquid chromatography-tandem mass spectrometry |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-031.pdf |
| work_keys_str_mv | AT linbinbinyangzhijinhuangxulianglinxiaolian simultaneousdeterminationofnineneonicotinoidinsecticidesandfivemetabolitesinmeatbyquecherscoupledwithultrahighperformanceliquidchromatographytandemmassspectrometry |