Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit
Fistula-in-ano (FIA, anal fistula) treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are a new frontier in the treatment of FIA. In this study, we established a FIA model of Japanese rabbit and evaluated the effect of four treatments on fistula h...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/6918090 |
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| author | Xiao Qin Peng Wang Yongming Huang Yansen Li Min Chao Wei Wang |
| author_facet | Xiao Qin Peng Wang Yongming Huang Yansen Li Min Chao Wei Wang |
| author_sort | Xiao Qin |
| collection | DOAJ |
| description | Fistula-in-ano (FIA, anal fistula) treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are a new frontier in the treatment of FIA. In this study, we established a FIA model of Japanese rabbit and evaluated the effect of four treatments on fistula healing: ADSC transplantation, acellular small intestinal submucosa (ASIS), noncutting seton, and PBS as negative control. High-throughput RNA sequencing was also performed to investigate the anal tissue (normal and ADSC treatment group) expression profile of mRNA. Our data showed that ADSC treatment had the shortest time to promote fistula healing compared to the other treatments, and fistula filled with new adipose tissue and muscle cells without scar. Gene Ontology (GO) analysis of RNA-seq data showed that the differential genes are enriched in system development and animal organ development. Taken together, our findings demonstrate that ADSCs rapidly promote fistula healing through differentiation, a promising stem cell therapy for FIA. The rabbit is an effective animal model for evaluating FIA therapeutic options. |
| format | Article |
| id | doaj-art-6644a7fd6d254daf80075813e9480fa4 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6644a7fd6d254daf80075813e9480fa42025-02-03T01:31:57ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/69180906918090Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese RabbitXiao Qin0Peng Wang1Yongming Huang2Yansen Li3Min Chao4Wei Wang5Department of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaDepartment of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272029 Shandong, ChinaFistula-in-ano (FIA, anal fistula) treatment remains a surgical challenge for coloproctologists. Adipose tissue-derived stem cells (ADSCs) are a new frontier in the treatment of FIA. In this study, we established a FIA model of Japanese rabbit and evaluated the effect of four treatments on fistula healing: ADSC transplantation, acellular small intestinal submucosa (ASIS), noncutting seton, and PBS as negative control. High-throughput RNA sequencing was also performed to investigate the anal tissue (normal and ADSC treatment group) expression profile of mRNA. Our data showed that ADSC treatment had the shortest time to promote fistula healing compared to the other treatments, and fistula filled with new adipose tissue and muscle cells without scar. Gene Ontology (GO) analysis of RNA-seq data showed that the differential genes are enriched in system development and animal organ development. Taken together, our findings demonstrate that ADSCs rapidly promote fistula healing through differentiation, a promising stem cell therapy for FIA. The rabbit is an effective animal model for evaluating FIA therapeutic options.http://dx.doi.org/10.1155/2019/6918090 |
| spellingShingle | Xiao Qin Peng Wang Yongming Huang Yansen Li Min Chao Wei Wang Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit Stem Cells International |
| title | Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit |
| title_full | Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit |
| title_fullStr | Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit |
| title_full_unstemmed | Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit |
| title_short | Adipose-Derived Stem Cells Are an Efficient Treatment for Fistula-in-ano of Japanese Rabbit |
| title_sort | adipose derived stem cells are an efficient treatment for fistula in ano of japanese rabbit |
| url | http://dx.doi.org/10.1155/2019/6918090 |
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