Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves
Cryptococcus gattii is an etiologic agent of cryptococcosis and a serious disease that affects immunocompromised and immunocompetent patients worldwide. The therapeutic arsenal used to treat cryptococcosis is limited to a few antifungal agents, and the ability of C. gattii to form biofilms may hinde...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/5764187 |
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| author | Adriana A. Almeida-Apolonio Wellinton J. Cupozak-Pinheiro Vagner M. Berres Fabiana G. S. Dantas Terezinha I. E. Svidzinski Kelly M. P. Oliveira Marilene R. Chang |
| author_facet | Adriana A. Almeida-Apolonio Wellinton J. Cupozak-Pinheiro Vagner M. Berres Fabiana G. S. Dantas Terezinha I. E. Svidzinski Kelly M. P. Oliveira Marilene R. Chang |
| author_sort | Adriana A. Almeida-Apolonio |
| collection | DOAJ |
| description | Cryptococcus gattii is an etiologic agent of cryptococcosis and a serious disease that affects immunocompromised and immunocompetent patients worldwide. The therapeutic arsenal used to treat cryptococcosis is limited to a few antifungal agents, and the ability of C. gattii to form biofilms may hinder treatment and decrease its susceptibility to antifungal agents. The objective of this study was to evaluate the antifungal and antibiofilm activities of an ethanolic extract of Cochlospermum regium (Schrank) Pilger leaves against C. gattii. The antifungal activity was assessed by measuring the minimum inhibitory concentration (MIC) using the broth microdilution technique and interaction of the extract with fluconazole was performed of checkerboard assay. The antibiofilm activity of the extract was evaluated in 96-well polystyrene microplates, and the biofilms were quantified by counting colony forming units. The extract showed antifungal activity at concentrations of 62.5 to 250 μg/mL and when the extract was evaluated in combination with fluconazole, C. gattii was inhibited at sub-MIC levels. The antibiofilm activity of the extract against C. gattii was observed both during biofilm formation and on an already established biofilm. The results showed that the ethanolic extract of the leaves of C. regium shows promise for the development of antifungal drugs to treat cryptococcosis and to combat C. gattii biofilms. |
| format | Article |
| id | doaj-art-663b826641204a1a8417497030a366b9 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-663b826641204a1a8417497030a366b92025-02-03T05:46:20ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/57641875764187Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger LeavesAdriana A. Almeida-Apolonio0Wellinton J. Cupozak-Pinheiro1Vagner M. Berres2Fabiana G. S. Dantas3Terezinha I. E. Svidzinski4Kelly M. P. Oliveira5Marilene R. Chang6Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, BrazilFaculdade de Ciências Exatas e Tecnologia, Universidade Federal da Grande Dourados, Dourados, MS 79804-970, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS 79804-970, BrazilFaculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS 79804-970, BrazilDepartamento de Análise Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR 87020-900, BrazilFaculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS 79804-970, BrazilFaculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, BrazilCryptococcus gattii is an etiologic agent of cryptococcosis and a serious disease that affects immunocompromised and immunocompetent patients worldwide. The therapeutic arsenal used to treat cryptococcosis is limited to a few antifungal agents, and the ability of C. gattii to form biofilms may hinder treatment and decrease its susceptibility to antifungal agents. The objective of this study was to evaluate the antifungal and antibiofilm activities of an ethanolic extract of Cochlospermum regium (Schrank) Pilger leaves against C. gattii. The antifungal activity was assessed by measuring the minimum inhibitory concentration (MIC) using the broth microdilution technique and interaction of the extract with fluconazole was performed of checkerboard assay. The antibiofilm activity of the extract was evaluated in 96-well polystyrene microplates, and the biofilms were quantified by counting colony forming units. The extract showed antifungal activity at concentrations of 62.5 to 250 μg/mL and when the extract was evaluated in combination with fluconazole, C. gattii was inhibited at sub-MIC levels. The antibiofilm activity of the extract against C. gattii was observed both during biofilm formation and on an already established biofilm. The results showed that the ethanolic extract of the leaves of C. regium shows promise for the development of antifungal drugs to treat cryptococcosis and to combat C. gattii biofilms.http://dx.doi.org/10.1155/2018/5764187 |
| spellingShingle | Adriana A. Almeida-Apolonio Wellinton J. Cupozak-Pinheiro Vagner M. Berres Fabiana G. S. Dantas Terezinha I. E. Svidzinski Kelly M. P. Oliveira Marilene R. Chang Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves The Scientific World Journal |
| title | Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves |
| title_full | Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves |
| title_fullStr | Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves |
| title_full_unstemmed | Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves |
| title_short | Control of Cryptococcus Gattii Biofilms by an Ethanolic Extract of Cochlospermum Regium (Schrank) Pilger Leaves |
| title_sort | control of cryptococcus gattii biofilms by an ethanolic extract of cochlospermum regium schrank pilger leaves |
| url | http://dx.doi.org/10.1155/2018/5764187 |
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