DNA barcoding: how many earthworm species are there in the south of West Siberia?
Earthworms are a widespread and ecologically important group of animals, which has the highest total biomass in some ecosystems and often defines the composition of soil fauna. Earthworms are known to have high cryptic genetic diversity. In this study we attempted to estimate earthworm species diver...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/471 |
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| author | S. V. Shekhovtsov N. E. Bazarova D. I. Berman N. A. Bulakhova E. V. Golovanova S. V. Konyaev T. M. Krugova I. I. Lyubechanskii S. E. Peltek |
| author_facet | S. V. Shekhovtsov N. E. Bazarova D. I. Berman N. A. Bulakhova E. V. Golovanova S. V. Konyaev T. M. Krugova I. I. Lyubechanskii S. E. Peltek |
| author_sort | S. V. Shekhovtsov |
| collection | DOAJ |
| description | Earthworms are a widespread and ecologically important group of animals, which has the highest total biomass in some ecosystems and often defines the composition of soil fauna. Earthworms are known to have high cryptic genetic diversity. In this study we attempted to estimate earthworm species diversity in the south of West Siberia by DNA barcoding. This method employs short fragments of the genome to identify species, and allows one to work with specimens that cannot be identified by conventional techniques, as well as to search for new species and predict their phylogenetic affinities. As the target sequence we took a fragment of the mitochondrial cytochrome oxidase 1 (cox1) gene. The studied territory (Novosibirsk and Tomsk oblasts, Altai krai, and the Altai Republic) is known to contain 16 species and subspecies of earthworms. We analyzed 259 individuals from twelve locations and detected 27 genetic clusters. Ten of them correspond to known species (A. caliginosa, E. fetida, O. tyrtaeum, D. r. tenuis, D. octaedra, E. balatonica, E. sibirica, as well as three genetic lineages of E. nordenskioldi nordenskioldi). Seventeen of the 27 clusters do not have close sequence similarity to any known earthworm species. Representatives of some of these novel clusters are morphologically similar to the Eisenia n. nordenskioldi/E. n. pallida species complex and may belong to new genetic lineages of this complex. The rest of the novel clusters probably represent new earthworm species. Therefore, we can conclude that a large portion of earthworm biodiversity in the south of West Siberia is still unexplored. |
| format | Article |
| id | doaj-art-6632323975c14b4dab9de649c17dc65f |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-6632323975c14b4dab9de649c17dc65f2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-03-0120112513010.18699/VJ15.110453DNA barcoding: how many earthworm species are there in the south of West Siberia?S. V. Shekhovtsov0N. E. Bazarova1D. I. Berman2N. A. Bulakhova3E. V. Golovanova4S. V. Konyaev5T. M. Krugova6I. I. Lyubechanskii7S. E. Peltek8Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk State Agricultural University, Novosibirsk, RussiaInstitute of Biological Problems of the North FEB RAS, Magadan, RussiaInstitute of Biological Problems of the North FEB RAS, Magadan, Russia Tomsk State University, Tomsk, RussiaOmsk State Paedagogical University, Omsk, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Novosibirsk, RussiaTigirek State Natural Reserve, Barnaul, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaEarthworms are a widespread and ecologically important group of animals, which has the highest total biomass in some ecosystems and often defines the composition of soil fauna. Earthworms are known to have high cryptic genetic diversity. In this study we attempted to estimate earthworm species diversity in the south of West Siberia by DNA barcoding. This method employs short fragments of the genome to identify species, and allows one to work with specimens that cannot be identified by conventional techniques, as well as to search for new species and predict their phylogenetic affinities. As the target sequence we took a fragment of the mitochondrial cytochrome oxidase 1 (cox1) gene. The studied territory (Novosibirsk and Tomsk oblasts, Altai krai, and the Altai Republic) is known to contain 16 species and subspecies of earthworms. We analyzed 259 individuals from twelve locations and detected 27 genetic clusters. Ten of them correspond to known species (A. caliginosa, E. fetida, O. tyrtaeum, D. r. tenuis, D. octaedra, E. balatonica, E. sibirica, as well as three genetic lineages of E. nordenskioldi nordenskioldi). Seventeen of the 27 clusters do not have close sequence similarity to any known earthworm species. Representatives of some of these novel clusters are morphologically similar to the Eisenia n. nordenskioldi/E. n. pallida species complex and may belong to new genetic lineages of this complex. The rest of the novel clusters probably represent new earthworm species. Therefore, we can conclude that a large portion of earthworm biodiversity in the south of West Siberia is still unexplored.https://vavilov.elpub.ru/jour/article/view/471earthwormslumbricidaewest siberiadna barcodingcox1cytochrome oxidase |
| spellingShingle | S. V. Shekhovtsov N. E. Bazarova D. I. Berman N. A. Bulakhova E. V. Golovanova S. V. Konyaev T. M. Krugova I. I. Lyubechanskii S. E. Peltek DNA barcoding: how many earthworm species are there in the south of West Siberia? Вавиловский журнал генетики и селекции earthworms lumbricidae west siberia dna barcoding cox1 cytochrome oxidase |
| title | DNA barcoding: how many earthworm species are there in the south of West Siberia? |
| title_full | DNA barcoding: how many earthworm species are there in the south of West Siberia? |
| title_fullStr | DNA barcoding: how many earthworm species are there in the south of West Siberia? |
| title_full_unstemmed | DNA barcoding: how many earthworm species are there in the south of West Siberia? |
| title_short | DNA barcoding: how many earthworm species are there in the south of West Siberia? |
| title_sort | dna barcoding how many earthworm species are there in the south of west siberia |
| topic | earthworms lumbricidae west siberia dna barcoding cox1 cytochrome oxidase |
| url | https://vavilov.elpub.ru/jour/article/view/471 |
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