Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma
Obesity is an established risk factor for breast cancer incidence and mortality. However, the mechanism that links obesity to tumorigenesis is not well understood. Here we combined nonlinear optical imaging technologies with an early-onset diet-induced obesity breast cancer animal model to evaluate...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2007-05-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2007.00018 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832544616973336576 |
|---|---|
| author | Thuc T. Le Charles W. Rehrer Terry B. Huff Maxine B. Nichols Ignacio G. Camarillo Ji-Xin Cheng |
| author_facet | Thuc T. Le Charles W. Rehrer Terry B. Huff Maxine B. Nichols Ignacio G. Camarillo Ji-Xin Cheng |
| author_sort | Thuc T. Le |
| collection | DOAJ |
| description | Obesity is an established risk factor for breast cancer incidence and mortality. However, the mechanism that links obesity to tumorigenesis is not well understood. Here we combined nonlinear optical imaging technologies with an early-onset diet-induced obesity breast cancer animal model to evaluate the impact of obesity on the composition of mammary gland and tumor stroma. Using coherent anti-Stokes Raman scattering and second harmonic generation on the same platform, we simultaneously imaged mammary adipocytes, blood capillaries, collagen fibrils, and tumor cells without any labeling. We observed that obesity increases the size of lipid droplets of adipocytes in mammary gland and collagen content in mammary tumor stroma, respectively. Such impacts of obesity on mammary gland and tumor stroma could not be analyzed using standard two-dimensional histologic evaluation. Given the importance of mammary stroma to the growth and migration of tumor cells, our observation provides the first imaging evidence that supports the relationship between obesity and breast cancer risk. |
| format | Article |
| id | doaj-art-660a6e0e3a2745c799a4c0a4f2300722 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-660a6e0e3a2745c799a4c0a4f23007222025-02-03T10:07:40ZengSAGE PublishingMolecular Imaging1536-01212007-05-01610.2310/7290.2007.0001810.2310_7290.2007.00018Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor StromaThuc T. LeCharles W. RehrerTerry B. HuffMaxine B. NicholsIgnacio G. CamarilloJi-Xin ChengObesity is an established risk factor for breast cancer incidence and mortality. However, the mechanism that links obesity to tumorigenesis is not well understood. Here we combined nonlinear optical imaging technologies with an early-onset diet-induced obesity breast cancer animal model to evaluate the impact of obesity on the composition of mammary gland and tumor stroma. Using coherent anti-Stokes Raman scattering and second harmonic generation on the same platform, we simultaneously imaged mammary adipocytes, blood capillaries, collagen fibrils, and tumor cells without any labeling. We observed that obesity increases the size of lipid droplets of adipocytes in mammary gland and collagen content in mammary tumor stroma, respectively. Such impacts of obesity on mammary gland and tumor stroma could not be analyzed using standard two-dimensional histologic evaluation. Given the importance of mammary stroma to the growth and migration of tumor cells, our observation provides the first imaging evidence that supports the relationship between obesity and breast cancer risk.https://doi.org/10.2310/7290.2007.00018 |
| spellingShingle | Thuc T. Le Charles W. Rehrer Terry B. Huff Maxine B. Nichols Ignacio G. Camarillo Ji-Xin Cheng Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma Molecular Imaging |
| title | Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma |
| title_full | Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma |
| title_fullStr | Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma |
| title_full_unstemmed | Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma |
| title_short | Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma |
| title_sort | nonlinear optical imaging to evaluate the impact of obesity on mammary gland and tumor stroma |
| url | https://doi.org/10.2310/7290.2007.00018 |
| work_keys_str_mv | AT thuctle nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma AT charleswrehrer nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma AT terrybhuff nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma AT maxinebnichols nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma AT ignaciogcamarillo nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma AT jixincheng nonlinearopticalimagingtoevaluatetheimpactofobesityonmammaryglandandtumorstroma |