Molecular Networks Involved in the Immune Control of BK Polyomavirus
BK polyomavirus infection is the important cause of virus-related nephropathy following kidney transplantation. BK virus reactivates in 30%–80% of kidney transplant recipients resulting in BK virus-related nephropathy in 1%–10% of cases. Currently, the molecular processes associated with asymptomati...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/972102 |
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| author | Eva Girmanova Irena Brabcova Jiri Klema Petra Hribova Mariana Wohlfartova Jelena Skibova Ondrej Viklicky |
| author_facet | Eva Girmanova Irena Brabcova Jiri Klema Petra Hribova Mariana Wohlfartova Jelena Skibova Ondrej Viklicky |
| author_sort | Eva Girmanova |
| collection | DOAJ |
| description | BK polyomavirus infection is the important cause of virus-related nephropathy following kidney transplantation. BK virus reactivates in 30%–80% of kidney transplant recipients resulting in BK virus-related nephropathy in 1%–10% of cases. Currently, the molecular processes associated with asymptomatic infections in transplant patients infected with BK virus remain unclear.
In this study we evaluate intrarenal molecular processes during different stages of BKV infection.
The gene expression profiles of 90 target genes known to be associated with immune response were evaluated in kidney graft biopsy material using TaqMan low density array. Three patient groups were examined: control patients with no evidence of BK virus reactivation (n=11), infected asymptomatic patients (n=9), and patients with BK virus nephropathy (n=10). Analysis of biopsies from asymptomatic viruria patients resulted in the identification of 5 differentially expressed genes (CD3E, CD68, CCR2, ICAM-1, and SKI) (P<0.05), and functional analysis showed a significantly heightened presence of costimulatory signals (e.g., CD40/CD40L; P<0.05). Gene ontology analysis revealed several biological networks associated with BKV immune control in comparison to the control group.
This study demonstrated that asymptomatic BK viruria is associated with a different intrarenal regulation of several genes implicating in antiviral immune response. |
| format | Article |
| id | doaj-art-65ef676ffeb042ab9c5317b24a5439a8 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-65ef676ffeb042ab9c5317b24a5439a82025-02-03T05:51:54ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/972102972102Molecular Networks Involved in the Immune Control of BK PolyomavirusEva Girmanova0Irena Brabcova1Jiri Klema2Petra Hribova3Mariana Wohlfartova4Jelena Skibova5Ondrej Viklicky6Transplant Laboratory, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicFaculty of Electrical Engineering, Czech Technical University in Prague, Karlovo namesti 13, 121 35 Prague 2, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicDepartment of Nephrology, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicDepartment of Statistics, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicTransplant Laboratory, Institute for Clinical and Experimental Medicine, Videnska 1958, 140 21 Prague 4, Czech RepublicBK polyomavirus infection is the important cause of virus-related nephropathy following kidney transplantation. BK virus reactivates in 30%–80% of kidney transplant recipients resulting in BK virus-related nephropathy in 1%–10% of cases. Currently, the molecular processes associated with asymptomatic infections in transplant patients infected with BK virus remain unclear. In this study we evaluate intrarenal molecular processes during different stages of BKV infection. The gene expression profiles of 90 target genes known to be associated with immune response were evaluated in kidney graft biopsy material using TaqMan low density array. Three patient groups were examined: control patients with no evidence of BK virus reactivation (n=11), infected asymptomatic patients (n=9), and patients with BK virus nephropathy (n=10). Analysis of biopsies from asymptomatic viruria patients resulted in the identification of 5 differentially expressed genes (CD3E, CD68, CCR2, ICAM-1, and SKI) (P<0.05), and functional analysis showed a significantly heightened presence of costimulatory signals (e.g., CD40/CD40L; P<0.05). Gene ontology analysis revealed several biological networks associated with BKV immune control in comparison to the control group. This study demonstrated that asymptomatic BK viruria is associated with a different intrarenal regulation of several genes implicating in antiviral immune response.http://dx.doi.org/10.1155/2012/972102 |
| spellingShingle | Eva Girmanova Irena Brabcova Jiri Klema Petra Hribova Mariana Wohlfartova Jelena Skibova Ondrej Viklicky Molecular Networks Involved in the Immune Control of BK Polyomavirus Clinical and Developmental Immunology |
| title | Molecular Networks Involved in the Immune Control of BK Polyomavirus |
| title_full | Molecular Networks Involved in the Immune Control of BK Polyomavirus |
| title_fullStr | Molecular Networks Involved in the Immune Control of BK Polyomavirus |
| title_full_unstemmed | Molecular Networks Involved in the Immune Control of BK Polyomavirus |
| title_short | Molecular Networks Involved in the Immune Control of BK Polyomavirus |
| title_sort | molecular networks involved in the immune control of bk polyomavirus |
| url | http://dx.doi.org/10.1155/2012/972102 |
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