Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses
Abstract Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia (Hymenochaetales) were validated. Many morphological characters of Fuscopori...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-023-00117-6 |
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| author | Yoonhee Cho Dohye Kim Yoongil Lee Juhwan Jeong Shahid Hussain Young Woon Lim |
| author_facet | Yoonhee Cho Dohye Kim Yoongil Lee Juhwan Jeong Shahid Hussain Young Woon Lim |
| author_sort | Yoonhee Cho |
| collection | DOAJ |
| description | Abstract Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia (Hymenochaetales) were validated. Many morphological characters of Fuscoporia overlap among the species, emphasizing the role of molecular identification for accuracy. The identities of 658 Fuscoporia GenBank internal transcribed spacer (ITS) sequences were assessed using ITS phylogeny, revealing 109 (16.6%) misidentified and 196 (29.8%) unspecified sequences. They were validated and re-identified based on the research articles they were published in and, if unpublished, based on sequences from the type, type locality-derived sequences, or otherwise reliable sequences. To enhance the resolution of species delimitation, a phylogenetic assessment of a multi-marker dataset (ITS + nrLSU + rpb2 + tef1) was conducted. The multi-marker phylogeny resolved five of the twelve species complexes found in the ITS phylogeny and uncovered five new Fuscoporia species: F. dolichoseta, F. gilvoides, F. koreana, F. reticulata, and F. semicephala. The validated ITS sequences in this study may prevent further accumulation of misidentified sequences in public databases and contribute to a more accurate taxonomic evaluation of Fuscoporia species. |
| format | Article |
| id | doaj-art-65e7b623b7724af89b05876ca5739ecd |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-65e7b623b7724af89b05876ca5739ecd2025-02-03T01:36:10ZengBMCIMA Fungus2210-63592023-06-0114112610.1186/s43008-023-00117-6Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analysesYoonhee Cho0Dohye Kim1Yoongil Lee2Juhwan Jeong3Shahid Hussain4Young Woon Lim5School of Biological Sciences and Institute of Microbiology, Seoul National UniversitySchool of Biological Sciences and Institute of Microbiology, Seoul National UniversitySchool of Biological Sciences and Institute of Microbiology, Seoul National UniversitySchool of Biological Sciences and Institute of Microbiology, Seoul National UniversityCentre for Plant Sciences and Biodiversity, University of SwatSchool of Biological Sciences and Institute of Microbiology, Seoul National UniversityAbstract Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia (Hymenochaetales) were validated. Many morphological characters of Fuscoporia overlap among the species, emphasizing the role of molecular identification for accuracy. The identities of 658 Fuscoporia GenBank internal transcribed spacer (ITS) sequences were assessed using ITS phylogeny, revealing 109 (16.6%) misidentified and 196 (29.8%) unspecified sequences. They were validated and re-identified based on the research articles they were published in and, if unpublished, based on sequences from the type, type locality-derived sequences, or otherwise reliable sequences. To enhance the resolution of species delimitation, a phylogenetic assessment of a multi-marker dataset (ITS + nrLSU + rpb2 + tef1) was conducted. The multi-marker phylogeny resolved five of the twelve species complexes found in the ITS phylogeny and uncovered five new Fuscoporia species: F. dolichoseta, F. gilvoides, F. koreana, F. reticulata, and F. semicephala. The validated ITS sequences in this study may prevent further accumulation of misidentified sequences in public databases and contribute to a more accurate taxonomic evaluation of Fuscoporia species.https://doi.org/10.1186/s43008-023-00117-6AnnotationFive new taxaHymenochaetaceaeITSMolecular identificationPhellinus |
| spellingShingle | Yoonhee Cho Dohye Kim Yoongil Lee Juhwan Jeong Shahid Hussain Young Woon Lim Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses IMA Fungus Annotation Five new taxa Hymenochaetaceae ITS Molecular identification Phellinus |
| title | Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses |
| title_full | Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses |
| title_fullStr | Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses |
| title_full_unstemmed | Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses |
| title_short | Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses |
| title_sort | validation of fuscoporia hymenochaetales basidiomycota its sequences and five new species based on multi marker phylogenetic and morphological analyses |
| topic | Annotation Five new taxa Hymenochaetaceae ITS Molecular identification Phellinus |
| url | https://doi.org/10.1186/s43008-023-00117-6 |
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