Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq
The study aimed to determine the infection rate in sheep, analyze the correlation between the rate and certain risk factors, identify larval stage distribution in visceral organs, and provide a molecular characterization of Cysticercus tenuicollis. Ninety-six slaughtered sheep and their offal were v...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2025-01-01
|
| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_186058_8c2bfb0dbe92d8d785b2652381205d8b.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540896506150912 |
|---|---|
| author | Omer K. Alhankawe Nadia S. Alhayali Donea A. Abdullah |
| author_facet | Omer K. Alhankawe Nadia S. Alhayali Donea A. Abdullah |
| author_sort | Omer K. Alhankawe |
| collection | DOAJ |
| description | The study aimed to determine the infection rate in sheep, analyze the correlation between the rate and certain risk factors, identify larval stage distribution in visceral organs, and provide a molecular characterization of Cysticercus tenuicollis. Ninety-six slaughtered sheep and their offal were visually inspected between February and July 2024. Sheep were categorized based on their sex and age. Cysts were observed in the diaphragm, liver, lung, mesentery, and omentum after the animals were slaughtered. The 12SrRNA gene was amplified and sequenced. For statistical analysis, the Chi-square test was applied. The overall infection rate was 16.66% (16/96). Males showed a higher rate 17.8% than females 13.04%, with a statistically significant. The rate was higher in adult males 21.56% and females 14.28% than in young males 9.09% and females 11.11%. March had the lowest rate 6.66%, and June had the highest 31.57%. The most common site of C. tenuicollis cyst was in the omentum 68.75%, followed by mesentery 31.25%. Successful 12SrRNA gene amplification was achieved in all 16 positive cases, and the amplified products were 490 bp. GenBank accession numbers were registered. Genetic relatedness revealed that the local C. tenuicollis isolates were more closely related to isolates from Sulaymaniyah and Iran. The findings showed that sheep are commonly infected with cysticercosis, which may result in financial losses due to organ condemnation. Thus, it is important to appropriately dispose of butcher materials as they could have contributed to the disease prevalence. |
| format | Article |
| id | doaj-art-65bb2426265341918030be23762b6c3f |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-65bb2426265341918030be23762b6c3f2025-02-04T13:11:42ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139110711410.33899/ijvs.2024.153622.3884186058Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, IraqOmer K. Alhankawe0Nadia S. Alhayali1Donea A. Abdullah2Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Animal Production Techniques, Northern Technical University, Mosul, IraqThe study aimed to determine the infection rate in sheep, analyze the correlation between the rate and certain risk factors, identify larval stage distribution in visceral organs, and provide a molecular characterization of Cysticercus tenuicollis. Ninety-six slaughtered sheep and their offal were visually inspected between February and July 2024. Sheep were categorized based on their sex and age. Cysts were observed in the diaphragm, liver, lung, mesentery, and omentum after the animals were slaughtered. The 12SrRNA gene was amplified and sequenced. For statistical analysis, the Chi-square test was applied. The overall infection rate was 16.66% (16/96). Males showed a higher rate 17.8% than females 13.04%, with a statistically significant. The rate was higher in adult males 21.56% and females 14.28% than in young males 9.09% and females 11.11%. March had the lowest rate 6.66%, and June had the highest 31.57%. The most common site of C. tenuicollis cyst was in the omentum 68.75%, followed by mesentery 31.25%. Successful 12SrRNA gene amplification was achieved in all 16 positive cases, and the amplified products were 490 bp. GenBank accession numbers were registered. Genetic relatedness revealed that the local C. tenuicollis isolates were more closely related to isolates from Sulaymaniyah and Iran. The findings showed that sheep are commonly infected with cysticercosis, which may result in financial losses due to organ condemnation. Thus, it is important to appropriately dispose of butcher materials as they could have contributed to the disease prevalence.https://www.vetmedmosul.com/article_186058_8c2bfb0dbe92d8d785b2652381205d8b.pdfcysticercosiscystsinfection ratepcrgenebank |
| spellingShingle | Omer K. Alhankawe Nadia S. Alhayali Donea A. Abdullah Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq Iraqi Journal of Veterinary Sciences cysticercosis cysts infection rate pcr genebank |
| title | Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq |
| title_full | Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq |
| title_fullStr | Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq |
| title_full_unstemmed | Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq |
| title_short | Molecular identification and phylogenetic analysis of Cysticercus tenuicollis isolated from sheep in Mosul city, Iraq |
| title_sort | molecular identification and phylogenetic analysis of cysticercus tenuicollis isolated from sheep in mosul city iraq |
| topic | cysticercosis cysts infection rate pcr genebank |
| url | https://www.vetmedmosul.com/article_186058_8c2bfb0dbe92d8d785b2652381205d8b.pdf |
| work_keys_str_mv | AT omerkalhankawe molecularidentificationandphylogeneticanalysisofcysticercustenuicollisisolatedfromsheepinmosulcityiraq AT nadiasalhayali molecularidentificationandphylogeneticanalysisofcysticercustenuicollisisolatedfromsheepinmosulcityiraq AT doneaaabdullah molecularidentificationandphylogeneticanalysisofcysticercustenuicollisisolatedfromsheepinmosulcityiraq |