Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213
In this study, the in vitro digestion and fermentation of two intra-polysaccharide fractions (IPS1 and IPS2) from Paecilomyces cicadae TJJ1213 were investigated. The constituent monosaccharides of IPS1 and IPS2 were not changed after simulated saliva, gastric and small intestinal digestion. However,...
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302200177X |
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| author | Juanjuan Tian Xiaomeng Wang Xueliang Zhang Xiaohong Chen Mingsheng Dong Xin Rui Qiuqin Zhang Mei Jiang Wei Li |
| author_facet | Juanjuan Tian Xiaomeng Wang Xueliang Zhang Xiaohong Chen Mingsheng Dong Xin Rui Qiuqin Zhang Mei Jiang Wei Li |
| author_sort | Juanjuan Tian |
| collection | DOAJ |
| description | In this study, the in vitro digestion and fermentation of two intra-polysaccharide fractions (IPS1 and IPS2) from Paecilomyces cicadae TJJ1213 were investigated. The constituent monosaccharides of IPS1 and IPS2 were not changed after simulated saliva, gastric and small intestinal digestion. However, they can be hydrolyzed and utilized by gut microbiota, and short-chain fatty acids (SCFAs) level were increased after IPS1and IPS2 treatments. Furthermore, 16S rRNA sequencing analysis of fermentation samples were performed. Alpha-diversity, beta-diversity and taxonomic composition differences analysis revealed that IPS1 and IPS2 promoted the proliferation of beneficial bacteria and modulated the overall structure of gut microbiota. Taxonomic comparison analysis found that IPS1 increased the relative abundances of beneficial bacteria including Megamonas, Bifidobacterium and Lactobacillus, while IPS2 could increase the abundance of Bacteroides, Parabacteroides and Phascolarctobacterium. In addition, they can also decrease the levels of pathogenic bacteria containing Escherichia-Shigella, Klebsiella and Fusobacterium. These results indicated that IPS from P. cicadae TJJ1213 could be used as potential candidates for new functional foods. |
| format | Article |
| id | doaj-art-65b4007c183b46acbc8e2aee77fdebea |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-65b4007c183b46acbc8e2aee77fdebea2025-02-03T04:06:19ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122622633Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213Juanjuan Tian0Xiaomeng Wang1Xueliang Zhang2Xiaohong Chen3Mingsheng Dong4Xin Rui5Qiuqin Zhang6Mei Jiang7Wei Li8College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCorresponding author at: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaIn this study, the in vitro digestion and fermentation of two intra-polysaccharide fractions (IPS1 and IPS2) from Paecilomyces cicadae TJJ1213 were investigated. The constituent monosaccharides of IPS1 and IPS2 were not changed after simulated saliva, gastric and small intestinal digestion. However, they can be hydrolyzed and utilized by gut microbiota, and short-chain fatty acids (SCFAs) level were increased after IPS1and IPS2 treatments. Furthermore, 16S rRNA sequencing analysis of fermentation samples were performed. Alpha-diversity, beta-diversity and taxonomic composition differences analysis revealed that IPS1 and IPS2 promoted the proliferation of beneficial bacteria and modulated the overall structure of gut microbiota. Taxonomic comparison analysis found that IPS1 increased the relative abundances of beneficial bacteria including Megamonas, Bifidobacterium and Lactobacillus, while IPS2 could increase the abundance of Bacteroides, Parabacteroides and Phascolarctobacterium. In addition, they can also decrease the levels of pathogenic bacteria containing Escherichia-Shigella, Klebsiella and Fusobacterium. These results indicated that IPS from P. cicadae TJJ1213 could be used as potential candidates for new functional foods.http://www.sciencedirect.com/science/article/pii/S221345302200177XPaecilomyces cicadae TJJ1213PolysaccharideFermentationGut microbiota |
| spellingShingle | Juanjuan Tian Xiaomeng Wang Xueliang Zhang Xiaohong Chen Mingsheng Dong Xin Rui Qiuqin Zhang Mei Jiang Wei Li Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 Food Science and Human Wellness Paecilomyces cicadae TJJ1213 Polysaccharide Fermentation Gut microbiota |
| title | Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 |
| title_full | Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 |
| title_fullStr | Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 |
| title_full_unstemmed | Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 |
| title_short | Artificial simulated saliva, gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 |
| title_sort | artificial simulated saliva gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from paecilomyces cicadae tjj1213 |
| topic | Paecilomyces cicadae TJJ1213 Polysaccharide Fermentation Gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S221345302200177X |
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