In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media
The molecular and mechanical characteristics of in situ degradation behavior of chitosan-polygalacturonic acid/hydroxyapatite (Chi-PgA-HAP) nanocomposite films is investigated using Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM), and modulus mapping techniques for up t...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2010/175264 |
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| author | Rohit Khanna Kalpana S. Katti Dinesh R. Katti |
| author_facet | Rohit Khanna Kalpana S. Katti Dinesh R. Katti |
| author_sort | Rohit Khanna |
| collection | DOAJ |
| description | The molecular and mechanical characteristics of in situ degradation behavior of chitosan-polygalacturonic acid/hydroxyapatite (Chi-PgA-HAP) nanocomposite films is investigated using Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM), and modulus mapping techniques for up to 48 days of soaking in cell culture media. The surface molecular structure of media-soaked samples changes over the course of 48 days of soaking, as indicated by significant changes in phosphate vibrations (1200–900 cm−1) indicating apatite formation. Chitosan-Polygalacturonic acid polyelectrolyte complexes (PECs) govern structural integrity of Chi-PgA-HAP nanocomposites and FTIR spectra indicate that PECs remain intact until 48 days of soaking. In situ AFM experiments on media-soaked samples indicate that soaking results in a change in topography and swelling proceeds differently at the initial soaking periods of about 8 days than for longer soaking. In situ modulus mapping experiments are done on soaked samples by probing ∼1–3 nm of surface indicating elastic moduli of ∼4 GPa resulting from proteins adsorbed on Chi-PgA-HAP nanocomposites. The elastic modulus decreases by ∼2 GPa over a long exposure to cell culture media (48 days). Thus, as water enters the Chi-PgA-HAP sample, surface molecular interactions in Chi-PgA-HAP structure occur that result in swelling, causing small changes in nanoscale mechanical properties. |
| format | Article |
| id | doaj-art-65b3aff9c624490b87257db0016deebe |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-65b3aff9c624490b87257db0016deebe2025-02-03T06:14:19ZengWileyInternational Journal of Polymer Science1687-94221687-94302010-01-01201010.1155/2010/175264175264In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture MediaRohit Khanna0Kalpana S. Katti1Dinesh R. Katti2Department of Civil Engineering, North Dakota State University, Fargo, ND 58105, USADepartment of Civil Engineering, North Dakota State University, Fargo, ND 58105, USADepartment of Civil Engineering, North Dakota State University, Fargo, ND 58105, USAThe molecular and mechanical characteristics of in situ degradation behavior of chitosan-polygalacturonic acid/hydroxyapatite (Chi-PgA-HAP) nanocomposite films is investigated using Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM), and modulus mapping techniques for up to 48 days of soaking in cell culture media. The surface molecular structure of media-soaked samples changes over the course of 48 days of soaking, as indicated by significant changes in phosphate vibrations (1200–900 cm−1) indicating apatite formation. Chitosan-Polygalacturonic acid polyelectrolyte complexes (PECs) govern structural integrity of Chi-PgA-HAP nanocomposites and FTIR spectra indicate that PECs remain intact until 48 days of soaking. In situ AFM experiments on media-soaked samples indicate that soaking results in a change in topography and swelling proceeds differently at the initial soaking periods of about 8 days than for longer soaking. In situ modulus mapping experiments are done on soaked samples by probing ∼1–3 nm of surface indicating elastic moduli of ∼4 GPa resulting from proteins adsorbed on Chi-PgA-HAP nanocomposites. The elastic modulus decreases by ∼2 GPa over a long exposure to cell culture media (48 days). Thus, as water enters the Chi-PgA-HAP sample, surface molecular interactions in Chi-PgA-HAP structure occur that result in swelling, causing small changes in nanoscale mechanical properties.http://dx.doi.org/10.1155/2010/175264 |
| spellingShingle | Rohit Khanna Kalpana S. Katti Dinesh R. Katti In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media International Journal of Polymer Science |
| title | In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media |
| title_full | In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media |
| title_fullStr | In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media |
| title_full_unstemmed | In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media |
| title_short | In Situ Swelling Behavior of Chitosan-Polygalacturonic Acid/Hydroxyapatite Nanocomposites in Cell Culture Media |
| title_sort | in situ swelling behavior of chitosan polygalacturonic acid hydroxyapatite nanocomposites in cell culture media |
| url | http://dx.doi.org/10.1155/2010/175264 |
| work_keys_str_mv | AT rohitkhanna insituswellingbehaviorofchitosanpolygalacturonicacidhydroxyapatitenanocompositesincellculturemedia AT kalpanaskatti insituswellingbehaviorofchitosanpolygalacturonicacidhydroxyapatitenanocompositesincellculturemedia AT dineshrkatti insituswellingbehaviorofchitosanpolygalacturonicacidhydroxyapatitenanocompositesincellculturemedia |