Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant
Data presented here demonstrate multifunctional vaccination strategies that harness vaccinia virus mediated delivery of a gene encoding an immunoenhanced diabetes autoantigen in combination with complete Freund’s adjuvant (CFA) that can maintain safe and durable immunologic homeostasis in NOD mice....
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/578786 |
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| author | Béla Dénes István Fodor William H. R. Langridge |
| author_facet | Béla Dénes István Fodor William H. R. Langridge |
| author_sort | Béla Dénes |
| collection | DOAJ |
| description | Data presented here demonstrate multifunctional vaccination strategies that harness vaccinia virus mediated delivery of a gene encoding an immunoenhanced diabetes autoantigen in combination with complete Freund’s adjuvant (CFA) that can maintain safe and durable immunologic homeostasis in NOD mice. Systemic coinoculation of prediabetic mice with recombinant vaccinia virus rVV-CTB::GAD and undiluted or 10-fold diluted CFA demonstrated a significant decrease in hyperglycemia and pancreatic islet inflammation in comparison with control animals during 17–61 and 17–105 weeks of age, respectively. Synergy in these beneficial effects was observed during 43–61 and 61–105 wks of age, respectively. Inflammatory cytokine and chemokine levels in GAD-stimulated splenocytes isolated from vaccinated mice were generally lower than those detected in unvaccinated mice. The overall health and humoral immune responses of the vaccinated animals remained normal throughout the duration of the experiments. |
| format | Article |
| id | doaj-art-659d17b734844ad6afa17a224baae502 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-659d17b734844ad6afa17a224baae5022025-02-03T05:53:47ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/578786578786Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s AdjuvantBéla Dénes0István Fodor1William H. R. Langridge2Center for Health Disparities and Molecular Medicine, Department of Biochemistry, School of Medicine, Loma Linda University, 11085 Campus Street, Loma Linda, CA 92350, USACenter for Health Disparities and Molecular Medicine, Department of Biochemistry, School of Medicine, Loma Linda University, 11085 Campus Street, Loma Linda, CA 92350, USACenter for Health Disparities and Molecular Medicine, Department of Biochemistry, School of Medicine, Loma Linda University, 11085 Campus Street, Loma Linda, CA 92350, USAData presented here demonstrate multifunctional vaccination strategies that harness vaccinia virus mediated delivery of a gene encoding an immunoenhanced diabetes autoantigen in combination with complete Freund’s adjuvant (CFA) that can maintain safe and durable immunologic homeostasis in NOD mice. Systemic coinoculation of prediabetic mice with recombinant vaccinia virus rVV-CTB::GAD and undiluted or 10-fold diluted CFA demonstrated a significant decrease in hyperglycemia and pancreatic islet inflammation in comparison with control animals during 17–61 and 17–105 weeks of age, respectively. Synergy in these beneficial effects was observed during 43–61 and 61–105 wks of age, respectively. Inflammatory cytokine and chemokine levels in GAD-stimulated splenocytes isolated from vaccinated mice were generally lower than those detected in unvaccinated mice. The overall health and humoral immune responses of the vaccinated animals remained normal throughout the duration of the experiments.http://dx.doi.org/10.1155/2013/578786 |
| spellingShingle | Béla Dénes István Fodor William H. R. Langridge Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant Clinical and Developmental Immunology |
| title | Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant |
| title_full | Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant |
| title_fullStr | Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant |
| title_full_unstemmed | Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant |
| title_short | Persistent Suppression of Type 1 Diabetes by a Multicomponent Vaccine Containing a Cholera Toxin B Subunit-Autoantigen Fusion Protein and Complete Freund’s Adjuvant |
| title_sort | persistent suppression of type 1 diabetes by a multicomponent vaccine containing a cholera toxin b subunit autoantigen fusion protein and complete freund s adjuvant |
| url | http://dx.doi.org/10.1155/2013/578786 |
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