Soy 11S Globulin Acid Subunits as the Novel Food Polymer Carrier
Aflatoxins were conjugated with soy 11S globulins acid subunits and the hapten-specific monoclonal antibodies (McAbs) cross-reactive with four major aflatoxins were achieved using indirect competitive ELIS...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2015/250146 |
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| Summary: | Aflatoxins were
conjugated with soy 11S globulins acid subunits
and the hapten-specific monoclonal
antibodies (McAbs) cross-reactive
with four major aflatoxins were achieved using
indirect competitive ELISA screening procedure.
The two antibodies (clones 1B2 and 2D3) had
similar reaction efficiency with aflatoxins B1,
B2, and G1 but showed a weak cross-reaction to
G2. The clone 4C5 exhibited the highest
sensitivity for all four aflatoxins. The
concentrations of aflatoxins B1, B2, G1, and G2 at
50% inhibition for 4C5 were 1.1, 1.2, 2.1,
and 17.6 pg mL−1. The
results indicated that soy 11S globulin acid
subunits were suitable novel carriers for
aflatoxin antigen in immunization experiments
and clone 4C5 could be used for simultaneous
analysis of total aflatoxins. |
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| ISSN: | 1687-9422 1687-9430 |