Characterization of Human Mesenchymal Stem Cells Isolated from the Testis
Mesenchymal stem cells hold great promise for regenerative medicine as they can be easily isolated from different sources such as adipose tissue, bone marrow, and umbilical cord blood. Spontaneously arising pluripotent stem cells can be obtained in culture from murine spermatogonial stem cells (SSCs...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/4910304 |
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| author | Letizia De Chiara Elvira Smeralda Famulari Sharmila Fagoonee Saskia K. M. van Daalen Stefano Buttiglieri Alberto Revelli Emanuela Tolosano Lorenzo Silengo Ans M. M. van Pelt Fiorella Altruda |
| author_facet | Letizia De Chiara Elvira Smeralda Famulari Sharmila Fagoonee Saskia K. M. van Daalen Stefano Buttiglieri Alberto Revelli Emanuela Tolosano Lorenzo Silengo Ans M. M. van Pelt Fiorella Altruda |
| author_sort | Letizia De Chiara |
| collection | DOAJ |
| description | Mesenchymal stem cells hold great promise for regenerative medicine as they can be easily isolated from different sources such as adipose tissue, bone marrow, and umbilical cord blood. Spontaneously arising pluripotent stem cells can be obtained in culture from murine spermatogonial stem cells (SSCs), while the pluripotency of the human counterpart remains a matter of debate. Recent gene expression profiling studies have demonstrated that embryonic stem cell- (ESC-) like cells obtained from the human testis are indeed closer to mesenchymal stem cells (MSCs) than to pluripotent stem cells. Here, we confirm that colonies derived from human testicular cultures, with our isolation protocol, are of mesenchymal origin and do not arise from spermatogonial stem cells (SSCs). The testis, thus, provides an important and accessible source of MSCs (tMSCs) that can be potentially used for nephrotoxicity testing in vitro. We further demonstrate, for the first time, that tMSCs are able to secrete microvesicles that could possibly be applied to the treatment of various chronic diseases, such as those affecting the kidney. |
| format | Article |
| id | doaj-art-659415803a0e4fbf994885f43924c753 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-659415803a0e4fbf994885f43924c7532025-02-03T05:50:34ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/49103044910304Characterization of Human Mesenchymal Stem Cells Isolated from the TestisLetizia De Chiara0Elvira Smeralda Famulari1Sharmila Fagoonee2Saskia K. M. van Daalen3Stefano Buttiglieri4Alberto Revelli5Emanuela Tolosano6Lorenzo Silengo7Ans M. M. van Pelt8Fiorella Altruda9Centro di Eccellenza DeNothe, Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Pieraccini 6, 50139 Firenze, ItalyMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyCenter for Reproductive Medicine, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, NetherlandsMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyObstetrics and Gynecology 1U, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, Sant’Anna Hospital, University of Turin, Corso Spezia 60, 10126 Turin, ItalyMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyCenter for Reproductive Medicine, Women’s and Children’s Hospital, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, NetherlandsMolecular Biotechnology Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, Via Nizza 52, 10126 Turin, ItalyMesenchymal stem cells hold great promise for regenerative medicine as they can be easily isolated from different sources such as adipose tissue, bone marrow, and umbilical cord blood. Spontaneously arising pluripotent stem cells can be obtained in culture from murine spermatogonial stem cells (SSCs), while the pluripotency of the human counterpart remains a matter of debate. Recent gene expression profiling studies have demonstrated that embryonic stem cell- (ESC-) like cells obtained from the human testis are indeed closer to mesenchymal stem cells (MSCs) than to pluripotent stem cells. Here, we confirm that colonies derived from human testicular cultures, with our isolation protocol, are of mesenchymal origin and do not arise from spermatogonial stem cells (SSCs). The testis, thus, provides an important and accessible source of MSCs (tMSCs) that can be potentially used for nephrotoxicity testing in vitro. We further demonstrate, for the first time, that tMSCs are able to secrete microvesicles that could possibly be applied to the treatment of various chronic diseases, such as those affecting the kidney.http://dx.doi.org/10.1155/2018/4910304 |
| spellingShingle | Letizia De Chiara Elvira Smeralda Famulari Sharmila Fagoonee Saskia K. M. van Daalen Stefano Buttiglieri Alberto Revelli Emanuela Tolosano Lorenzo Silengo Ans M. M. van Pelt Fiorella Altruda Characterization of Human Mesenchymal Stem Cells Isolated from the Testis Stem Cells International |
| title | Characterization of Human Mesenchymal Stem Cells Isolated from the Testis |
| title_full | Characterization of Human Mesenchymal Stem Cells Isolated from the Testis |
| title_fullStr | Characterization of Human Mesenchymal Stem Cells Isolated from the Testis |
| title_full_unstemmed | Characterization of Human Mesenchymal Stem Cells Isolated from the Testis |
| title_short | Characterization of Human Mesenchymal Stem Cells Isolated from the Testis |
| title_sort | characterization of human mesenchymal stem cells isolated from the testis |
| url | http://dx.doi.org/10.1155/2018/4910304 |
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