Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens
We investigated the virulence gene expression of carbapenem-resistant Acinetobacter baumanii (CRAB) isolated from the respiratory samples of patients with CRAB pneumonia and those with CRAB colonization to identify the virulence genes contributing to CRAB pneumonia’s development and mortality. Patie...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/8265683 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558532777476096 |
|---|---|
| author | Ji Yun Bae Ina Yun Kang Il Jun Chung-Jong Kim Miae Lee Hee Jung Choi |
| author_facet | Ji Yun Bae Ina Yun Kang Il Jun Chung-Jong Kim Miae Lee Hee Jung Choi |
| author_sort | Ji Yun Bae |
| collection | DOAJ |
| description | We investigated the virulence gene expression of carbapenem-resistant Acinetobacter baumanii (CRAB) isolated from the respiratory samples of patients with CRAB pneumonia and those with CRAB colonization to identify the virulence genes contributing to CRAB pneumonia’s development and mortality. Patients with CRAB identified from respiratory specimens were screened at a tertiary university hospital between January 2018 and January 2019. Patients were classified into CRAB pneumonia or CRAB colonization groups according to predefined clinical criteria. A. baumannii isolated from respiratory specimens was examined for the expression levels of ompA, uspA, hfq, hisF, feoA, and bfnL by quantitative reverse-transcription polymerase chain reaction. Among 156 patients with CRAB from respiratory specimens, 17 and 24 met the criteria for inclusion in the pneumonia and colonization groups, respectively. The expression level of ompA was significantly higher in the pneumonia group than in the colonization group (1.45 vs. 0.63, P=0.03). The expression levels of ompA (1.97 vs. 0.86, P=0.02), hisF (1.06 vs. 0.10, P<0.01), uspA (1.62 vs. 1.01, P<0.01), and bfnL (3.14 vs. 2.14, P=0.03) were significantly higher in patients with 30-day mortality than in the surviving patients. Elevated expression of hisF (adjusted odds ratio = 5.93, P=0.03) and uspA (adjusted odds ratio = 7.36, P=0.02) were associated with 30-day mortality after adjusting for age and the Charlson score. uspA and hisF may serve as putative targets for novel therapeutic strategies. |
| format | Article |
| id | doaj-art-658282ffd8114cccab7af10677a35e6e |
| institution | Kabale University |
| issn | 1918-1493 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-658282ffd8114cccab7af10677a35e6e2025-02-03T01:32:07ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1918-14932023-01-01202310.1155/2023/8265683Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical SpecimensJi Yun Bae0Ina Yun1Kang Il Jun2Chung-Jong Kim3Miae Lee4Hee Jung Choi5Department of Internal MedicineEwha Education and Research Center for InfectionEwha Education and Research Center for InfectionEwha Education and Research Center for InfectionEwha Education and Research Center for InfectionDepartment of Internal MedicineWe investigated the virulence gene expression of carbapenem-resistant Acinetobacter baumanii (CRAB) isolated from the respiratory samples of patients with CRAB pneumonia and those with CRAB colonization to identify the virulence genes contributing to CRAB pneumonia’s development and mortality. Patients with CRAB identified from respiratory specimens were screened at a tertiary university hospital between January 2018 and January 2019. Patients were classified into CRAB pneumonia or CRAB colonization groups according to predefined clinical criteria. A. baumannii isolated from respiratory specimens was examined for the expression levels of ompA, uspA, hfq, hisF, feoA, and bfnL by quantitative reverse-transcription polymerase chain reaction. Among 156 patients with CRAB from respiratory specimens, 17 and 24 met the criteria for inclusion in the pneumonia and colonization groups, respectively. The expression level of ompA was significantly higher in the pneumonia group than in the colonization group (1.45 vs. 0.63, P=0.03). The expression levels of ompA (1.97 vs. 0.86, P=0.02), hisF (1.06 vs. 0.10, P<0.01), uspA (1.62 vs. 1.01, P<0.01), and bfnL (3.14 vs. 2.14, P=0.03) were significantly higher in patients with 30-day mortality than in the surviving patients. Elevated expression of hisF (adjusted odds ratio = 5.93, P=0.03) and uspA (adjusted odds ratio = 7.36, P=0.02) were associated with 30-day mortality after adjusting for age and the Charlson score. uspA and hisF may serve as putative targets for novel therapeutic strategies.http://dx.doi.org/10.1155/2023/8265683 |
| spellingShingle | Ji Yun Bae Ina Yun Kang Il Jun Chung-Jong Kim Miae Lee Hee Jung Choi Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens |
| title_full | Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens |
| title_fullStr | Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens |
| title_full_unstemmed | Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens |
| title_short | Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens |
| title_sort | association between pneumonia development and virulence gene expression in carbapenem resistant acinetobacter baumannii isolated from clinical specimens |
| url | http://dx.doi.org/10.1155/2023/8265683 |
| work_keys_str_mv | AT jiyunbae associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens AT inayun associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens AT kangiljun associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens AT chungjongkim associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens AT miaelee associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens AT heejungchoi associationbetweenpneumoniadevelopmentandvirulencegeneexpressionincarbapenemresistantacinetobacterbaumanniiisolatedfromclinicalspecimens |