Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran
The live strain of Brucella melitensis Rev1 is recognised as the available vaccine for controlling brucellosis in small ruminants. This study aimed to assess the humoral immune responses in sheep when administered the native Rev.1 vaccine. All animals were inoculated with the Rev.1 vaccine, and bloo...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2025-03-01
|
| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832581898930487296 |
|---|---|
| author | J. Gharekhani E. Barati M. Dadar |
| author_facet | J. Gharekhani E. Barati M. Dadar |
| author_sort | J. Gharekhani |
| collection | DOAJ |
| description | The live strain of Brucella melitensis Rev1 is recognised as the available vaccine for controlling brucellosis in small ruminants. This study aimed to assess the humoral immune responses in sheep when administered the native Rev.1 vaccine. All animals were inoculated with the Rev.1 vaccine, and blood samples were collected on days 0, 14, 28, 44, 55, and 69 post-vaccination. These samples were tested using the modified Rose Bengal plate test (mRBPT), Wright test, 2-mercaptoethanol (2-ME) test, and enzyme-linked immunosorbent assay (ELISA) techniques. After six rounds of sampling, all animals tested negative in the mRBPT evaluation, and the control group exhibited low levels of anti-bodies to brucellosis when using ELISA. In the test group, most cases of positive mRBPT results were observed on days 14 and 28 of sampling. The Wright, 2-ME, and ELISA tests revealed that the highest antibody levels were detected 14 days after vaccination. In contrast to the Wright and 2-ME findings, the level of antibodies continued to rise until the 44th day after vaccination when using ELISA. The results demonstrated the stimulation of humoral immune responses in the target animals through the native vaccine, which could be employed to immunise livestock in endemic areas like Iran. The ELISA test proved to be more effective than conventional tests in revealing the serological response induced by Rev.1 vaccination, although ELISA may not reliably distinguish between anti-bodies generated by the vaccine and those resulting from the disease. |
| format | Article |
| id | doaj-art-657ff00f05ef468cb6daf3eb1d2f76b6 |
| institution | Kabale University |
| issn | 1311-1477 1313-3543 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj-art-657ff00f05ef468cb6daf3eb1d2f76b62025-01-30T08:33:11ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432025-03-0128113314410.15547/bjvm.2023-0117Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from IranJ. Gharekhani0E. Barati 1M. Dadar2Central Veterinary Laboratory, Iranian Veterinary Organization, Hamedan, IranCentral Veterinary Laboratory, Iranian Veterinary Organization, Hamedan, IranRazi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranThe live strain of Brucella melitensis Rev1 is recognised as the available vaccine for controlling brucellosis in small ruminants. This study aimed to assess the humoral immune responses in sheep when administered the native Rev.1 vaccine. All animals were inoculated with the Rev.1 vaccine, and blood samples were collected on days 0, 14, 28, 44, 55, and 69 post-vaccination. These samples were tested using the modified Rose Bengal plate test (mRBPT), Wright test, 2-mercaptoethanol (2-ME) test, and enzyme-linked immunosorbent assay (ELISA) techniques. After six rounds of sampling, all animals tested negative in the mRBPT evaluation, and the control group exhibited low levels of anti-bodies to brucellosis when using ELISA. In the test group, most cases of positive mRBPT results were observed on days 14 and 28 of sampling. The Wright, 2-ME, and ELISA tests revealed that the highest antibody levels were detected 14 days after vaccination. In contrast to the Wright and 2-ME findings, the level of antibodies continued to rise until the 44th day after vaccination when using ELISA. The results demonstrated the stimulation of humoral immune responses in the target animals through the native vaccine, which could be employed to immunise livestock in endemic areas like Iran. The ELISA test proved to be more effective than conventional tests in revealing the serological response induced by Rev.1 vaccination, although ELISA may not reliably distinguish between anti-bodies generated by the vaccine and those resulting from the disease.brucellosiselisarev.1 vaccine2- mercaptoethanol testwright test |
| spellingShingle | J. Gharekhani E. Barati M. Dadar Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran Bulgarian Journal of Veterinary Medicine brucellosis elisa rev.1 vaccine 2- mercaptoethanol test wright test |
| title | Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran |
| title_full | Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran |
| title_fullStr | Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran |
| title_full_unstemmed | Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran |
| title_short | Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran |
| title_sort | evaluation of the humoral immune responses of brucellosis vaccine rev 1 in sheep a study from iran |
| topic | brucellosis elisa rev.1 vaccine 2- mercaptoethanol test wright test |
| work_keys_str_mv | AT jgharekhani evaluationofthehumoralimmuneresponsesofbrucellosisvaccinerev1insheepastudyfromiran AT ebarati evaluationofthehumoralimmuneresponsesofbrucellosisvaccinerev1insheepastudyfromiran AT mdadar evaluationofthehumoralimmuneresponsesofbrucellosisvaccinerev1insheepastudyfromiran |