Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs

A study on florfenicol (FF) and florfenicol amine (FFA) residue depletion raws was conducted in eggs. After the chickens were administered successively florfenicol (FF) powder of 15 mg/kg (prevention group) and 30 mg/kg (treatment group) of body mass in the form of premix for 5 d, the eggs of each g...

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Main Authors: ZHANG Jing, ZHOU Qian, GAO Yushi, TANG Xiujun, LU Junxian, MA Lina, CHEN Dawei, TANG Mengjun
Format: Article
Language:English
Published: Zhejiang University Press 2021-01-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.03.241
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author ZHANG Jing
ZHOU Qian
GAO Yushi
TANG Xiujun
LU Junxian
MA Lina
CHEN Dawei
TANG Mengjun
author_facet ZHANG Jing
ZHOU Qian
GAO Yushi
TANG Xiujun
LU Junxian
MA Lina
CHEN Dawei
TANG Mengjun
author_sort ZHANG Jing
collection DOAJ
description A study on florfenicol (FF) and florfenicol amine (FFA) residue depletion raws was conducted in eggs. After the chickens were administered successively florfenicol (FF) powder of 15 mg/kg (prevention group) and 30 mg/kg (treatment group) of body mass in the form of premix for 5 d, the eggs of each group were collected at administration period and 1, 2, 3, 5, 7, 10, 12, 15, 18 d at withdrawal period. Then FF and FFA residues of egg, egg yolk and egg white were detected using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the limits of detection (LOD) of FF and FFA in egg, egg yolk and egg white were 2 μg/kg, and the limits of quantitation (LOQ) were 5 μg/kg. The average recoveries were in the range of 78.9%-105.0% when the egg samples were added with FF in the range of 1.0-5.0 μg/kg, and the intraday precision and the interday precision was 2.22%-4.22% and 4.22%-6.54%, respectively. The peak values of FF residues (520.70, 797.12 μg/kg) of egg white in prevention group and treatment group, respectively, were higher than those of egg yolk (285.96, 442.40 μg/kg), while the peak values of FFA residue (218.40, 646.14 μg/kg) of egg yolk in prevention group and treatment group, respectively, were higher than those of egg white (87.36, 36.89 μg/kg). The elimination rates of FF and FFA in egg white were faster than those in egg yolk. FF and FFA residues and their total residues were positively correlated with the dosage. FF and FFA mainly existed in the egg yolk, and FF mainly existed in the egg white. The FF residual time of egg yolk in different dosage groups was 18 d.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-656c4a2d6fc84aa5b6ffd27d674bbdfd2025-08-20T02:47:40ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552021-01-014712713410.3785/j.issn.1008-9209.2020.03.24110089209Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggsZHANG JingZHOU QianGAO YushiTANG XiujunLU JunxianMA LinaCHEN DaweiTANG MengjunA study on florfenicol (FF) and florfenicol amine (FFA) residue depletion raws was conducted in eggs. After the chickens were administered successively florfenicol (FF) powder of 15 mg/kg (prevention group) and 30 mg/kg (treatment group) of body mass in the form of premix for 5 d, the eggs of each group were collected at administration period and 1, 2, 3, 5, 7, 10, 12, 15, 18 d at withdrawal period. Then FF and FFA residues of egg, egg yolk and egg white were detected using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the limits of detection (LOD) of FF and FFA in egg, egg yolk and egg white were 2 μg/kg, and the limits of quantitation (LOQ) were 5 μg/kg. The average recoveries were in the range of 78.9%-105.0% when the egg samples were added with FF in the range of 1.0-5.0 μg/kg, and the intraday precision and the interday precision was 2.22%-4.22% and 4.22%-6.54%, respectively. The peak values of FF residues (520.70, 797.12 μg/kg) of egg white in prevention group and treatment group, respectively, were higher than those of egg yolk (285.96, 442.40 μg/kg), while the peak values of FFA residue (218.40, 646.14 μg/kg) of egg yolk in prevention group and treatment group, respectively, were higher than those of egg white (87.36, 36.89 μg/kg). The elimination rates of FF and FFA in egg white were faster than those in egg yolk. FF and FFA residues and their total residues were positively correlated with the dosage. FF and FFA mainly existed in the egg yolk, and FF mainly existed in the egg white. The FF residual time of egg yolk in different dosage groups was 18 d.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.03.241florfenicolflorfenicol amineeggresidue depletionhigh performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
spellingShingle ZHANG Jing
ZHOU Qian
GAO Yushi
TANG Xiujun
LU Junxian
MA Lina
CHEN Dawei
TANG Mengjun
Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
浙江大学学报. 农业与生命科学版
florfenicol
florfenicol amine
egg
residue depletion
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
title Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
title_full Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
title_fullStr Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
title_full_unstemmed Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
title_short Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
title_sort residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs
topic florfenicol
florfenicol amine
egg
residue depletion
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.03.241
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