Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants
The present study employed a <sup>65</sup>Zn radioactive isotope as a tracer to investigate the foliar uptake and distribution patterns of ZnO concentrated suspension- and ZnSO<sub>4</sub> solution-sprayed on soybean plant leaves. The radiolabeled foliar treatments were spray...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/15/2/197 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589471916228608 |
|---|---|
| author | Anita Beltrame João Paulo Rodrigues Marques Mariana Ayres Rodrigues Eduardo de Almeida Márcio Arruda Bacchi Elisabete Aparecida De Nadai Fernandes Rafael Otto Hudson Wallace Pereira de Carvalho |
| author_facet | Anita Beltrame João Paulo Rodrigues Marques Mariana Ayres Rodrigues Eduardo de Almeida Márcio Arruda Bacchi Elisabete Aparecida De Nadai Fernandes Rafael Otto Hudson Wallace Pereira de Carvalho |
| author_sort | Anita Beltrame |
| collection | DOAJ |
| description | The present study employed a <sup>65</sup>Zn radioactive isotope as a tracer to investigate the foliar uptake and distribution patterns of ZnO concentrated suspension- and ZnSO<sub>4</sub> solution-sprayed on soybean plant leaves. The radiolabeled foliar treatments were sprayed on the leaves at V4 and V8 phenological stages. The radioactivity of <sup>65</sup>Zn in the leaves, roots, stems, and pods was determined using γ-ray spectrometry. After the first foliar spray, V4, the partition of radiolabeled Zn in plants treated with ZnO and ZnSO<sub>4</sub> was 99.22% and 98.12% in treated leaves, 0.15% and 0.39% in stems, 0.16% and 0.29% in roots, and 0.47% and 1.19% in newly expanded non-treated leaves, respectively. After two sprayings, V4 and V8, the partition of radiolabeled Zn in plants treated with ZnO and ZnSO<sub>4</sub> was 92.56% and 92.18% in treated leaves, 0.92% and 0.70% in stems, 0.52% and 0.39% in roots, 5.60% and 6.15% in newly expanded non-treated leaves, and 0.43% and 0.61% in grains, respectively. The total fraction translocated from the application tissue was 0.79% and 1.91% for ZnO and ZnSO<sub>4</sub>, respectively, after 12 days and 8.03% and 8.48% for ZnO and ZnSO<sub>4</sub>, respectively, after 72 days. An anatomical analysis revealed that plants cultivated in a nutrition solution with 10% ionic strength had 63% fewer stomata, and the xylem vessels were 63% smaller compared to plants grown in a solution with 100% Zn ionic. One can conclude that after a short period, 12 days, the absorption and translocation of ZnSO<sub>4</sub> was higher and faster than ZnO, and after the long period, 72 days, their performance was similar. |
| format | Article |
| id | doaj-art-65636b8dbb3943749fdf0e071e43598c |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-65636b8dbb3943749fdf0e071e43598c2025-01-24T13:16:05ZengMDPI AGAgriculture2077-04722025-01-0115219710.3390/agriculture15020197Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean PlantsAnita Beltrame0João Paulo Rodrigues Marques1Mariana Ayres Rodrigues2Eduardo de Almeida3Márcio Arruda Bacchi4Elisabete Aparecida De Nadai Fernandes5Rafael Otto6Hudson Wallace Pereira de Carvalho7Department of Soil Science, “Luiz de Queiroz” College of Agriculture, University of São Paulo, 11 Paduas Dias Avenue, Piracicaba 13418-900, SP, BrazilDepartment of Basic Sciences, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Soil Science, “Luiz de Queiroz” College of Agriculture, University of São Paulo, 11 Paduas Dias Avenue, Piracicaba 13418-900, SP, BrazilCenter for Nuclear Energy in Agriculture (CENA), University of São Paulo, Piracicaba 13416-000, SP, BrazilCenter for Nuclear Energy in Agriculture (CENA), University of São Paulo, Piracicaba 13416-000, SP, BrazilCenter for Nuclear Energy in Agriculture (CENA), University of São Paulo, Piracicaba 13416-000, SP, BrazilDepartment of Soil Science, “Luiz de Queiroz” College of Agriculture, University of São Paulo, 11 Paduas Dias Avenue, Piracicaba 13418-900, SP, BrazilCenter for Nuclear Energy in Agriculture (CENA), University of São Paulo, Piracicaba 13416-000, SP, BrazilThe present study employed a <sup>65</sup>Zn radioactive isotope as a tracer to investigate the foliar uptake and distribution patterns of ZnO concentrated suspension- and ZnSO<sub>4</sub> solution-sprayed on soybean plant leaves. The radiolabeled foliar treatments were sprayed on the leaves at V4 and V8 phenological stages. The radioactivity of <sup>65</sup>Zn in the leaves, roots, stems, and pods was determined using γ-ray spectrometry. After the first foliar spray, V4, the partition of radiolabeled Zn in plants treated with ZnO and ZnSO<sub>4</sub> was 99.22% and 98.12% in treated leaves, 0.15% and 0.39% in stems, 0.16% and 0.29% in roots, and 0.47% and 1.19% in newly expanded non-treated leaves, respectively. After two sprayings, V4 and V8, the partition of radiolabeled Zn in plants treated with ZnO and ZnSO<sub>4</sub> was 92.56% and 92.18% in treated leaves, 0.92% and 0.70% in stems, 0.52% and 0.39% in roots, 5.60% and 6.15% in newly expanded non-treated leaves, and 0.43% and 0.61% in grains, respectively. The total fraction translocated from the application tissue was 0.79% and 1.91% for ZnO and ZnSO<sub>4</sub>, respectively, after 12 days and 8.03% and 8.48% for ZnO and ZnSO<sub>4</sub>, respectively, after 72 days. An anatomical analysis revealed that plants cultivated in a nutrition solution with 10% ionic strength had 63% fewer stomata, and the xylem vessels were 63% smaller compared to plants grown in a solution with 100% Zn ionic. One can conclude that after a short period, 12 days, the absorption and translocation of ZnSO<sub>4</sub> was higher and faster than ZnO, and after the long period, 72 days, their performance was similar.https://www.mdpi.com/2077-0472/15/2/197<sup>65</sup>ZnZnSO<sub>4</sub>ZnOfoliar sprayleaf anatomy |
| spellingShingle | Anita Beltrame João Paulo Rodrigues Marques Mariana Ayres Rodrigues Eduardo de Almeida Márcio Arruda Bacchi Elisabete Aparecida De Nadai Fernandes Rafael Otto Hudson Wallace Pereira de Carvalho Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants Agriculture <sup>65</sup>Zn ZnSO<sub>4</sub> ZnO foliar spray leaf anatomy |
| title | Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants |
| title_full | Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants |
| title_fullStr | Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants |
| title_full_unstemmed | Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants |
| title_short | Foliar Application and Translocation of Radiolabeled Zinc Oxide Suspension vs. Zinc Sulfate Solution by Soybean Plants |
| title_sort | foliar application and translocation of radiolabeled zinc oxide suspension vs zinc sulfate solution by soybean plants |
| topic | <sup>65</sup>Zn ZnSO<sub>4</sub> ZnO foliar spray leaf anatomy |
| url | https://www.mdpi.com/2077-0472/15/2/197 |
| work_keys_str_mv | AT anitabeltrame foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT joaopaulorodriguesmarques foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT marianaayresrodrigues foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT eduardodealmeida foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT marcioarrudabacchi foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT elisabeteaparecidadenadaifernandes foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT rafaelotto foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants AT hudsonwallacepereiradecarvalho foliarapplicationandtranslocationofradiolabeledzincoxidesuspensionvszincsulfatesolutionbysoybeanplants |