Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media
Citric acid was produced with free and k-carrageenan-entrapped cells of the yeast Yarrowia lipolytica in single and repeated batch-shake-flask fermentations on glycerol-based media. Simultaneous solubilization of hydroxyapatite of animal bone origin (HABO) was tested in all experiments. The highest...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/907143 |
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| author | Maria Vassileva Bettina Eichler-Lobermann Antonia Reyes Nikolay Vassilev |
| author_facet | Maria Vassileva Bettina Eichler-Lobermann Antonia Reyes Nikolay Vassilev |
| author_sort | Maria Vassileva |
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| description | Citric acid was produced with free and k-carrageenan-entrapped cells of the yeast Yarrowia lipolytica in single and repeated batch-shake-flask fermentations on glycerol-based media. Simultaneous solubilization of hydroxyapatite of animal bone origin (HABO) was tested in all experiments. The highest citric acid production by free yeast cells of 20.4 g/L and 18.7 g/L was reached after 96 h of fermentation in the absence and presence of 3 g/L HABO, respectively. The maximum values for the same parameter achieved by gel-entrapped cells in conditions of single batch and repeated-batch fermentation processes were 18.7 g/L and 28.1 g/L registered after 96 h and the 3d batch cycle, respectively. The highest citric acid productivity of 0.58 g L−1 h−1 was obtained with immobilized cells in repeated batch mode of fermentation when the added hydroxyapatite of 3 g/L was solubilized to 399 mg/L whereas the maximum efficiency of 89.0% was obtained with 1 g/L of HABO. |
| format | Article |
| id | doaj-art-6559e03225f0497aa6a5a8284b157ff5 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6559e03225f0497aa6a5a8284b157ff52025-02-03T06:14:12ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/907143907143Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based MediaMaria Vassileva0Bettina Eichler-Lobermann1Antonia Reyes2Nikolay Vassilev3Department of Chemical Engineering, Faculty of Sciences, University of Granada, c/Fuentenueva s/n, 18071 Granada, SpainFaculty of Agricultural and Environmental Sciences, University of Rostock, 18051 Rostock, GermanyDepartment of Chemical Engineering, Faculty of Sciences, University of Granada, c/Fuentenueva s/n, 18071 Granada, SpainDepartment of Chemical Engineering, Faculty of Sciences, University of Granada, c/Fuentenueva s/n, 18071 Granada, SpainCitric acid was produced with free and k-carrageenan-entrapped cells of the yeast Yarrowia lipolytica in single and repeated batch-shake-flask fermentations on glycerol-based media. Simultaneous solubilization of hydroxyapatite of animal bone origin (HABO) was tested in all experiments. The highest citric acid production by free yeast cells of 20.4 g/L and 18.7 g/L was reached after 96 h of fermentation in the absence and presence of 3 g/L HABO, respectively. The maximum values for the same parameter achieved by gel-entrapped cells in conditions of single batch and repeated-batch fermentation processes were 18.7 g/L and 28.1 g/L registered after 96 h and the 3d batch cycle, respectively. The highest citric acid productivity of 0.58 g L−1 h−1 was obtained with immobilized cells in repeated batch mode of fermentation when the added hydroxyapatite of 3 g/L was solubilized to 399 mg/L whereas the maximum efficiency of 89.0% was obtained with 1 g/L of HABO.http://dx.doi.org/10.1100/2012/907143 |
| spellingShingle | Maria Vassileva Bettina Eichler-Lobermann Antonia Reyes Nikolay Vassilev Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media The Scientific World Journal |
| title | Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media |
| title_full | Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media |
| title_fullStr | Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media |
| title_full_unstemmed | Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media |
| title_short | Animal Bones Char Solubilization by Gel-Entrapped Yarrowia lipolytica on Glycerol-Based Media |
| title_sort | animal bones char solubilization by gel entrapped yarrowia lipolytica on glycerol based media |
| url | http://dx.doi.org/10.1100/2012/907143 |
| work_keys_str_mv | AT mariavassileva animalbonescharsolubilizationbygelentrappedyarrowialipolyticaonglycerolbasedmedia AT bettinaeichlerlobermann animalbonescharsolubilizationbygelentrappedyarrowialipolyticaonglycerolbasedmedia AT antoniareyes animalbonescharsolubilizationbygelentrappedyarrowialipolyticaonglycerolbasedmedia AT nikolayvassilev animalbonescharsolubilizationbygelentrappedyarrowialipolyticaonglycerolbasedmedia |