Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer
IntroductionSpecifically regulating endogenous molecules is a potential molecular therapeutic strategy. Naturally occurring circular RNAs (circRNAs) are structurally stable and have been proved to serve as highly efficient miR-sponges and protein-sponges in cancer cells.MethodsWe chemically synthesi...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2025.1443571/full |
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| author | Chujin Ye Chujin Ye Zhiye Liu Zhiye Liu Qifan Xie Qifan Xie Yanlin Tang Yanlin Tang Jiayi Zeng Jiayi Zeng Ziwei Feng Ziwei Feng Jiumin Liu Jiumin Liu Haibiao Xie Haibiao Xie |
| author_facet | Chujin Ye Chujin Ye Zhiye Liu Zhiye Liu Qifan Xie Qifan Xie Yanlin Tang Yanlin Tang Jiayi Zeng Jiayi Zeng Ziwei Feng Ziwei Feng Jiumin Liu Jiumin Liu Haibiao Xie Haibiao Xie |
| author_sort | Chujin Ye |
| collection | DOAJ |
| description | IntroductionSpecifically regulating endogenous molecules is a potential molecular therapeutic strategy. Naturally occurring circular RNAs (circRNAs) are structurally stable and have been proved to serve as highly efficient miR-sponges and protein-sponges in cancer cells.MethodsWe chemically synthesized circRNA (ScircRNA) in vitro to achieve therapeutic dysfunction by targeting specific miRNAs. RNase R and fetal bovine serum were used to evaluate the stability of ScircRNAs. In prostate cancer cell lines, the competitive inhibition of the ScircRNA on miR-375 and miR-21 activity was evaluated using luciferase report gene, cell proliferation, and apoptosis assays.ResultsWe found that ScircRNAs were more resistant to nuclease digestion and more effective inhibiting target miRNAs than linear RNA sponges. The ScircRNAs inhibited malignant phenotype of prostate cancer by specifically inhibiting the activity of miR-21 and miR-375. In addition, we used the ScircRNA inhibiting CDK5 expression to trigger T-cell mediated cancer immunotherapy for treating prostate cancer in vivo.DiscussionThe ScircRNAs possessed the advantages of stable structure and simple construction, and can specifically inhibit the function of target miRNAs, which has a potential therapeutic application prospect in prostate cancer. |
| format | Article |
| id | doaj-art-654388200999460fb28fb879fdb85903 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-654388200999460fb28fb879fdb859032025-01-31T06:40:04ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-01-011510.3389/fonc.2025.14435711443571Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancerChujin Ye0Chujin Ye1Zhiye Liu2Zhiye Liu3Qifan Xie4Qifan Xie5Yanlin Tang6Yanlin Tang7Jiayi Zeng8Jiayi Zeng9Ziwei Feng10Ziwei Feng11Jiumin Liu12Jiumin Liu13Haibiao Xie14Haibiao Xie15Department of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, ChinaThe Second School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaIntroductionSpecifically regulating endogenous molecules is a potential molecular therapeutic strategy. Naturally occurring circular RNAs (circRNAs) are structurally stable and have been proved to serve as highly efficient miR-sponges and protein-sponges in cancer cells.MethodsWe chemically synthesized circRNA (ScircRNA) in vitro to achieve therapeutic dysfunction by targeting specific miRNAs. RNase R and fetal bovine serum were used to evaluate the stability of ScircRNAs. In prostate cancer cell lines, the competitive inhibition of the ScircRNA on miR-375 and miR-21 activity was evaluated using luciferase report gene, cell proliferation, and apoptosis assays.ResultsWe found that ScircRNAs were more resistant to nuclease digestion and more effective inhibiting target miRNAs than linear RNA sponges. The ScircRNAs inhibited malignant phenotype of prostate cancer by specifically inhibiting the activity of miR-21 and miR-375. In addition, we used the ScircRNA inhibiting CDK5 expression to trigger T-cell mediated cancer immunotherapy for treating prostate cancer in vivo.DiscussionThe ScircRNAs possessed the advantages of stable structure and simple construction, and can specifically inhibit the function of target miRNAs, which has a potential therapeutic application prospect in prostate cancer.https://www.frontiersin.org/articles/10.3389/fonc.2025.1443571/fullprostate cancermiRNAcircRNAcancer immunotherapysynthetic biology |
| spellingShingle | Chujin Ye Chujin Ye Zhiye Liu Zhiye Liu Qifan Xie Qifan Xie Yanlin Tang Yanlin Tang Jiayi Zeng Jiayi Zeng Ziwei Feng Ziwei Feng Jiumin Liu Jiumin Liu Haibiao Xie Haibiao Xie Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer Frontiers in Oncology prostate cancer miRNA circRNA cancer immunotherapy synthetic biology |
| title | Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer |
| title_full | Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer |
| title_fullStr | Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer |
| title_full_unstemmed | Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer |
| title_short | Adeno-associated virus mediated artificial circular RNA for triggering cancer immunotherapy to treat prostate cancer |
| title_sort | adeno associated virus mediated artificial circular rna for triggering cancer immunotherapy to treat prostate cancer |
| topic | prostate cancer miRNA circRNA cancer immunotherapy synthetic biology |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2025.1443571/full |
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