Construction and evaluation of Brassica rapa orphan genes overexpression library
Orphan genes (OGs) are crucial for species-specific characteristics and stress responses and are restricted to a specific taxon. However, their functions within particular species are poorly understood. Previous research identified OGs in Brassica rapa (BrOGs). In this study, the BrOGs overexpressio...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1532449/full |
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| author | Mingliang Jiang Mingliang Jiang Zongxiang Zhan Xiaonan Li Zhongyun Piao |
| author_facet | Mingliang Jiang Mingliang Jiang Zongxiang Zhan Xiaonan Li Zhongyun Piao |
| author_sort | Mingliang Jiang |
| collection | DOAJ |
| description | Orphan genes (OGs) are crucial for species-specific characteristics and stress responses and are restricted to a specific taxon. However, their functions within particular species are poorly understood. Previous research identified OGs in Brassica rapa (BrOGs). In this study, the BrOGs overexpression (BrOGsOE) library in Arabidopsis thaliana was constructed. Approximately 128 unknown functional BrOGs were selected from Chinese cabbage and were overexpressed. The analysis focused on the phenotypes of leaf morphology and flowering time against phenotypic differences between Chinese cabbage and Arabidopsis. Interestingly, 72.66% of the transgenic lines showed distinctive phenotypic changes. Chinese cabbage-specific features, including curved, hairy, upward or downward-curving leaves, serrated margins, and multiple leaves, were observed in the BrOGsOE lines. The BrOGs overexpression library was associated with numerous variations in flowering time, particularly delayed flowering. This suggested that the delayed flowering time caused by BrOGs may be associated with resistance to bolting seem in Chinese cabbage. Furthermore, the results of stress treatment of 24 BrOGsOE lines with no apparent significant phenotypes suggested that a number of BrOGs have both general and specific functions against environmental and pathogenic stress. The findings of this study provide a comprehensive overview of the roles of BrOGs, emphasizing their significance as a resource for identifying positive genes associated with species-specific characteristics and stress responses and offering a solid foundation for the functional analysis of BrOGs. |
| format | Article |
| id | doaj-art-65283e8404154eb29571c82a35dc2d51 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-65283e8404154eb29571c82a35dc2d512025-01-22T07:15:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011610.3389/fpls.2025.15324491532449Construction and evaluation of Brassica rapa orphan genes overexpression libraryMingliang Jiang0Mingliang Jiang1Zongxiang Zhan2Xiaonan Li3Zhongyun Piao4Molecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaSchool of Agriculture, Jilin Agricultural Science and Technology University, Jilin, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaMolecular Biology of Vegetable Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaOrphan genes (OGs) are crucial for species-specific characteristics and stress responses and are restricted to a specific taxon. However, their functions within particular species are poorly understood. Previous research identified OGs in Brassica rapa (BrOGs). In this study, the BrOGs overexpression (BrOGsOE) library in Arabidopsis thaliana was constructed. Approximately 128 unknown functional BrOGs were selected from Chinese cabbage and were overexpressed. The analysis focused on the phenotypes of leaf morphology and flowering time against phenotypic differences between Chinese cabbage and Arabidopsis. Interestingly, 72.66% of the transgenic lines showed distinctive phenotypic changes. Chinese cabbage-specific features, including curved, hairy, upward or downward-curving leaves, serrated margins, and multiple leaves, were observed in the BrOGsOE lines. The BrOGs overexpression library was associated with numerous variations in flowering time, particularly delayed flowering. This suggested that the delayed flowering time caused by BrOGs may be associated with resistance to bolting seem in Chinese cabbage. Furthermore, the results of stress treatment of 24 BrOGsOE lines with no apparent significant phenotypes suggested that a number of BrOGs have both general and specific functions against environmental and pathogenic stress. The findings of this study provide a comprehensive overview of the roles of BrOGs, emphasizing their significance as a resource for identifying positive genes associated with species-specific characteristics and stress responses and offering a solid foundation for the functional analysis of BrOGs.https://www.frontiersin.org/articles/10.3389/fpls.2025.1532449/fullBrassica rapaorphan genesoverexpression libraryconstructionevaluation |
| spellingShingle | Mingliang Jiang Mingliang Jiang Zongxiang Zhan Xiaonan Li Zhongyun Piao Construction and evaluation of Brassica rapa orphan genes overexpression library Frontiers in Plant Science Brassica rapa orphan genes overexpression library construction evaluation |
| title | Construction and evaluation of Brassica rapa orphan genes overexpression library |
| title_full | Construction and evaluation of Brassica rapa orphan genes overexpression library |
| title_fullStr | Construction and evaluation of Brassica rapa orphan genes overexpression library |
| title_full_unstemmed | Construction and evaluation of Brassica rapa orphan genes overexpression library |
| title_short | Construction and evaluation of Brassica rapa orphan genes overexpression library |
| title_sort | construction and evaluation of brassica rapa orphan genes overexpression library |
| topic | Brassica rapa orphan genes overexpression library construction evaluation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1532449/full |
| work_keys_str_mv | AT mingliangjiang constructionandevaluationofbrassicarapaorphangenesoverexpressionlibrary AT mingliangjiang constructionandevaluationofbrassicarapaorphangenesoverexpressionlibrary AT zongxiangzhan constructionandevaluationofbrassicarapaorphangenesoverexpressionlibrary AT xiaonanli constructionandevaluationofbrassicarapaorphangenesoverexpressionlibrary AT zhongyunpiao constructionandevaluationofbrassicarapaorphangenesoverexpressionlibrary |