Plasticity of Cells and Ex Vivo Production of Red Blood Cells
The supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If transfusable RBCs could be produced abundantly from certain resources, it would be very useful. Our group has developed a method to produce enucleated RBCs efficiently from hematopoietic stem/progenitor cells p...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/195780 |
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| author | Takashi Hiroyama Kenichi Miharada Ryo Kurita Yukio Nakamura |
| author_facet | Takashi Hiroyama Kenichi Miharada Ryo Kurita Yukio Nakamura |
| author_sort | Takashi Hiroyama |
| collection | DOAJ |
| description | The supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If transfusable RBCs could be produced abundantly from certain resources, it would be very useful. Our group has developed a method to produce enucleated RBCs efficiently from hematopoietic stem/progenitor cells present in umbilical cord blood. More recently, it was reported that enucleated RBCs could be abundantly produced from human embryonic stem (ES) cells. The common obstacle for application of these methods is that they require very high cost to produce sufficient number of RBCs that are applicable in the clinic. If erythroid cell lines (immortalized cell lines) able to produce transfusable RBCs ex vivo were established, they would be valuable resources. Our group developed a robust method to obtain immortalized erythroid cell lines able to produce mature RBCs. To the best of our knowledge, this was the first paper to show the feasibility of establishing immortalized erythroid progenitor cell lines able to produce enucleated RBCs ex vivo. This result strongly suggests that immortalized human erythroid progenitor cell lines able to produce mature RBCs ex vivo can also be established. |
| format | Article |
| id | doaj-art-6523dde188ab4ab0bb2dfe50f32e8d2e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6523dde188ab4ab0bb2dfe50f32e8d2e2025-02-03T06:10:57ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/195780195780Plasticity of Cells and Ex Vivo Production of Red Blood CellsTakashi Hiroyama0Kenichi Miharada1Ryo Kurita2Yukio Nakamura3Cell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, JapanCell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, JapanCell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, JapanCell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, JapanThe supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If transfusable RBCs could be produced abundantly from certain resources, it would be very useful. Our group has developed a method to produce enucleated RBCs efficiently from hematopoietic stem/progenitor cells present in umbilical cord blood. More recently, it was reported that enucleated RBCs could be abundantly produced from human embryonic stem (ES) cells. The common obstacle for application of these methods is that they require very high cost to produce sufficient number of RBCs that are applicable in the clinic. If erythroid cell lines (immortalized cell lines) able to produce transfusable RBCs ex vivo were established, they would be valuable resources. Our group developed a robust method to obtain immortalized erythroid cell lines able to produce mature RBCs. To the best of our knowledge, this was the first paper to show the feasibility of establishing immortalized erythroid progenitor cell lines able to produce enucleated RBCs ex vivo. This result strongly suggests that immortalized human erythroid progenitor cell lines able to produce mature RBCs ex vivo can also be established.http://dx.doi.org/10.4061/2011/195780 |
| spellingShingle | Takashi Hiroyama Kenichi Miharada Ryo Kurita Yukio Nakamura Plasticity of Cells and Ex Vivo Production of Red Blood Cells Stem Cells International |
| title | Plasticity of Cells and Ex Vivo Production of Red Blood Cells |
| title_full | Plasticity of Cells and Ex Vivo Production of Red Blood Cells |
| title_fullStr | Plasticity of Cells and Ex Vivo Production of Red Blood Cells |
| title_full_unstemmed | Plasticity of Cells and Ex Vivo Production of Red Blood Cells |
| title_short | Plasticity of Cells and Ex Vivo Production of Red Blood Cells |
| title_sort | plasticity of cells and ex vivo production of red blood cells |
| url | http://dx.doi.org/10.4061/2011/195780 |
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