Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles
Whole bacterial cell characterization is critically important for fast bacterial identification. Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal. In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2012/297560 |
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| author | Mustafa Çulha M. Müge Yazıcı Mehmet Kahraman Fikrettin Şahin Sesin Kocagöz |
| author_facet | Mustafa Çulha M. Müge Yazıcı Mehmet Kahraman Fikrettin Şahin Sesin Kocagöz |
| author_sort | Mustafa Çulha |
| collection | DOAJ |
| description | Whole bacterial cell characterization is critically important for fast bacterial identification. Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal. In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal silver nanoparticles (AgNPs) with “convective-assembly” method is reported. The proper size of the microwells for the model bacteria, Escherichia coli and Staphylococcus cohnii, is determined to be 1.2 μm from their electron microscopy images. A minimum dilution factor of 20 is necessary for the bacterial samples collected from growth media to diminish the bacterial aggregation to place the bacterial cells into the microwells. The constructed microwell structures are tested for their bacterial SERS performance and compared to the SERS spectra obtained from the samples prepared with a simple mixing of bacteria and AgNPs for the same bacteria. The results indicate that microwell structures not only improve the spectral quality but also increase the reproducibility of the SERS spectra. |
| format | Article |
| id | doaj-art-6486d4e4594a4bf49a9c714449f1d8fe |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-6486d4e4594a4bf49a9c714449f1d8fe2025-02-03T05:47:51ZengWileyJournal of Nanotechnology1687-95031687-95112012-01-01201210.1155/2012/297560297560Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver NanoparticlesMustafa Çulha0M. Müge Yazıcı1Mehmet Kahraman2Fikrettin Şahin3Sesin Kocagöz4Genetics and Bioengineering Department, Faculty of Engineering and Architecture, Yeditepe University, Istanbul 34755, TurkeyGenetics and Bioengineering Department, Faculty of Engineering and Architecture, Yeditepe University, Istanbul 34755, TurkeyGenetics and Bioengineering Department, Faculty of Engineering and Architecture, Yeditepe University, Istanbul 34755, TurkeyGenetics and Bioengineering Department, Faculty of Engineering and Architecture, Yeditepe University, Istanbul 34755, TurkeyAcıbadem University, School of Medicine, Department of Infectious Diseases, Büyükdere Cad. No. 40, Maslak, Istanbul 34457, TurkeyWhole bacterial cell characterization is critically important for fast bacterial identification. Surface-enhanced Raman scattering (SERS) is proven to be powerful technique to serve such a goal. In this study, the characterization of whole bacterial cells in the microwells constructed from colloidal silver nanoparticles (AgNPs) with “convective-assembly” method is reported. The proper size of the microwells for the model bacteria, Escherichia coli and Staphylococcus cohnii, is determined to be 1.2 μm from their electron microscopy images. A minimum dilution factor of 20 is necessary for the bacterial samples collected from growth media to diminish the bacterial aggregation to place the bacterial cells into the microwells. The constructed microwell structures are tested for their bacterial SERS performance and compared to the SERS spectra obtained from the samples prepared with a simple mixing of bacteria and AgNPs for the same bacteria. The results indicate that microwell structures not only improve the spectral quality but also increase the reproducibility of the SERS spectra.http://dx.doi.org/10.1155/2012/297560 |
| spellingShingle | Mustafa Çulha M. Müge Yazıcı Mehmet Kahraman Fikrettin Şahin Sesin Kocagöz Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles Journal of Nanotechnology |
| title | Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles |
| title_full | Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles |
| title_fullStr | Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles |
| title_full_unstemmed | Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles |
| title_short | Surface-Enhanced Raman Scattering of Bacteria in Microwells Constructed from Silver Nanoparticles |
| title_sort | surface enhanced raman scattering of bacteria in microwells constructed from silver nanoparticles |
| url | http://dx.doi.org/10.1155/2012/297560 |
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