Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy
Temporal lobe epilepsy (TLE) is a chronic neurological disorder that is often resistant to antiepileptic drugs. The pathogenesis of TLE is extremely complicated and remains elusive. Understanding the molecular mechanisms underlying TLE is crucial for its diagnosis and treatment. In the present study...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2022/8511066 |
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| author | Xu Qian Ji-Qiang Ding Xin Zhao Xin-Wen Sheng Zhao-Rui Wang Qi-xing Yang Jing-Jun Zheng Jia-Gui Zhong Teng-Yue Zhang Shu-Qiao He Wei-Dong Ji Wei Li Mei Zhang |
| author_facet | Xu Qian Ji-Qiang Ding Xin Zhao Xin-Wen Sheng Zhao-Rui Wang Qi-xing Yang Jing-Jun Zheng Jia-Gui Zhong Teng-Yue Zhang Shu-Qiao He Wei-Dong Ji Wei Li Mei Zhang |
| author_sort | Xu Qian |
| collection | DOAJ |
| description | Temporal lobe epilepsy (TLE) is a chronic neurological disorder that is often resistant to antiepileptic drugs. The pathogenesis of TLE is extremely complicated and remains elusive. Understanding the molecular mechanisms underlying TLE is crucial for its diagnosis and treatment. In the present study, a lithium-pilocarpine-induced TLE model was employed to reveal the pathological changes of hippocampus in rats. Hippocampal samples were taken for proteomic analysis at 2 weeks after the onset of spontaneous seizure (a chronic stage of epileptogenesis). Isobaric tag for relative and absolute quantization (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS) technique was applied for proteomic analysis of hippocampus. A total of 4173 proteins were identified from the hippocampi of epileptic rats and its control, of which 27 differentially expressed proteins (DEPs) were obtained with a fold change>1.5 and P<0.05. Bioinformatics analysis indicated 27 DEPs were mainly enriched in “regulation of synaptic plasticity and structure” and “calmodulin-dependent protein kinase activity,” which implicate synaptic remodeling may play a vital role in the pathogenesis of TLE. Consequently, the synaptic plasticity-related proteins and synaptic structure were investigated to verify it. It has been demonstrated that CaMKII-α, CaMKII-β, and GFAP were significant upregulated coincidently with proteomic analysis in the hippocampus of TLE rats. Moreover, the increased dendritic spines and hippocampal sclerosis further proved that synaptic plasticity involves in the development of TLE. The present study may help to understand the molecular mechanisms underlying epileptogenesis and provide a basis for further studies on synaptic plasticity in TLE. |
| format | Article |
| id | doaj-art-646d5ac1f53b4a5bad781a5d16939f5d |
| institution | Kabale University |
| issn | 1687-5443 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-646d5ac1f53b4a5bad781a5d16939f5d2025-02-03T06:04:49ZengWileyNeural Plasticity1687-54432022-01-01202210.1155/2022/8511066Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe EpilepsyXu Qian0Ji-Qiang Ding1Xin Zhao2Xin-Wen Sheng3Zhao-Rui Wang4Qi-xing Yang5Jing-Jun Zheng6Jia-Gui Zhong7Teng-Yue Zhang8Shu-Qiao He9Wei-Dong Ji10Wei Li11Mei Zhang12Department of Clinical PharmacologyDepartment of NeurosurgeryDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of NeurosurgeryDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyDepartment of NeurosurgeryDepartment of Clinical PharmacologyDepartment of Clinical PharmacologyCenter for Translational MedicineDepartment of NeurosurgeryDepartment of Clinical PharmacologyTemporal lobe epilepsy (TLE) is a chronic neurological disorder that is often resistant to antiepileptic drugs. The pathogenesis of TLE is extremely complicated and remains elusive. Understanding the molecular mechanisms underlying TLE is crucial for its diagnosis and treatment. In the present study, a lithium-pilocarpine-induced TLE model was employed to reveal the pathological changes of hippocampus in rats. Hippocampal samples were taken for proteomic analysis at 2 weeks after the onset of spontaneous seizure (a chronic stage of epileptogenesis). Isobaric tag for relative and absolute quantization (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS) technique was applied for proteomic analysis of hippocampus. A total of 4173 proteins were identified from the hippocampi of epileptic rats and its control, of which 27 differentially expressed proteins (DEPs) were obtained with a fold change>1.5 and P<0.05. Bioinformatics analysis indicated 27 DEPs were mainly enriched in “regulation of synaptic plasticity and structure” and “calmodulin-dependent protein kinase activity,” which implicate synaptic remodeling may play a vital role in the pathogenesis of TLE. Consequently, the synaptic plasticity-related proteins and synaptic structure were investigated to verify it. It has been demonstrated that CaMKII-α, CaMKII-β, and GFAP were significant upregulated coincidently with proteomic analysis in the hippocampus of TLE rats. Moreover, the increased dendritic spines and hippocampal sclerosis further proved that synaptic plasticity involves in the development of TLE. The present study may help to understand the molecular mechanisms underlying epileptogenesis and provide a basis for further studies on synaptic plasticity in TLE.http://dx.doi.org/10.1155/2022/8511066 |
| spellingShingle | Xu Qian Ji-Qiang Ding Xin Zhao Xin-Wen Sheng Zhao-Rui Wang Qi-xing Yang Jing-Jun Zheng Jia-Gui Zhong Teng-Yue Zhang Shu-Qiao He Wei-Dong Ji Wei Li Mei Zhang Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy Neural Plasticity |
| title | Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy |
| title_full | Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy |
| title_fullStr | Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy |
| title_full_unstemmed | Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy |
| title_short | Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy |
| title_sort | proteomic analysis reveals the vital role of synaptic plasticity in the pathogenesis of temporal lobe epilepsy |
| url | http://dx.doi.org/10.1155/2022/8511066 |
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