Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation
Regulatory T cells (Tregs) play a crucial role in immune homeostasis. Treg induction is a strategy that parasites have evolved to modulate the host’s inflammatory environment, facilitating their establishment and permanence. In human Taenia solium neurocysticercosis (NC), the concurrence of increase...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/981468 |
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| author | Laura Adalid-Peralta Asiel Arce-Sillas Gladis Fragoso Graciela Cárdenas Marcos Rosetti Didier Casanova-Hernández Claudia Rangel-Escareño Laura Uribe-Figueroa Agnes Fleury Edda Sciutto |
| author_facet | Laura Adalid-Peralta Asiel Arce-Sillas Gladis Fragoso Graciela Cárdenas Marcos Rosetti Didier Casanova-Hernández Claudia Rangel-Escareño Laura Uribe-Figueroa Agnes Fleury Edda Sciutto |
| author_sort | Laura Adalid-Peralta |
| collection | DOAJ |
| description | Regulatory T cells (Tregs) play a crucial role in immune homeostasis. Treg induction is a strategy that parasites have evolved to modulate the host’s inflammatory environment, facilitating their establishment and permanence. In human Taenia solium neurocysticercosis (NC), the concurrence of increased peripheral and central Treg levels and their capacity to inhibit T cell activation and proliferation support their role in controlling neuroinflammation. This study is aimed at identifing possible mechanisms of Treg induction in human NC. Monocyte-derived dendritic cells (DC) from healthy human donors, cocultivated with autologous CD4+ naïve cells either in the presence or absence of cysticerci, promoted CD25highFoxp3+ Treg differentiation. An increased Treg induction was observed when cysticerci were present. Moreover, an augmentation of suppressive-related molecules (SLAMF1, B7-H1, and CD205) was found in parasite-induced DC differentiation. Increased Tregs and a higher in vivo DC expression of the regulatory molecules SLAMF1 and CD205 in NC patients were also found. SLAMF1 gene was downregulated in NC patients with extraparenchymal cysticerci, exhibiting higher inflammation levels than patients with parenchymal parasites. Our findings suggest that cysticerci may modulate DC to favor a suppressive environment, which may help parasite establishment, minimizing the excessive inflammation, which may lead to tissue damage. |
| format | Article |
| id | doaj-art-645fa6d354e24b93898b5b555aa49136 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-645fa6d354e24b93898b5b555aa491362025-02-03T01:20:59ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/981468981468Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs DifferentiationLaura Adalid-Peralta0Asiel Arce-Sillas1Gladis Fragoso2Graciela Cárdenas3Marcos Rosetti4Didier Casanova-Hernández5Claudia Rangel-Escareño6Laura Uribe-Figueroa7Agnes Fleury8Edda Sciutto9Instituto Nacional de Neurología y Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoUnidad Periférica para el Estudio de Neuroinflamación del Instituto de Investigaciones Biomédicas de la UNAM en el Instituto Nacional de Neurología Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoInstituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 México, DF, MexicoInstituto Nacional de Neurología y Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoInstituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 México, DF, MexicoUnidad Periférica para el Estudio de Neuroinflamación del Instituto de Investigaciones Biomédicas de la UNAM en el Instituto Nacional de Neurología Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoDepartamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, INMEGEN. Periférico Sur 4809, Arenal Tepepan, 14610 México, DF, MexicoUnidad de Genotipificación y Análisis de Expresión Affymetrix, INMEGEN, Periférico Sur 4809, Arenal Tepepan, 14610 México, DF, MexicoInstituto Nacional de Neurología y Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoUnidad Periférica para el Estudio de Neuroinflamación del Instituto de Investigaciones Biomédicas de la UNAM en el Instituto Nacional de Neurología Neurocirugía, Insurgentes Sur 3877, Col. La Fama, 14269 México, DF, MexicoRegulatory T cells (Tregs) play a crucial role in immune homeostasis. Treg induction is a strategy that parasites have evolved to modulate the host’s inflammatory environment, facilitating their establishment and permanence. In human Taenia solium neurocysticercosis (NC), the concurrence of increased peripheral and central Treg levels and their capacity to inhibit T cell activation and proliferation support their role in controlling neuroinflammation. This study is aimed at identifing possible mechanisms of Treg induction in human NC. Monocyte-derived dendritic cells (DC) from healthy human donors, cocultivated with autologous CD4+ naïve cells either in the presence or absence of cysticerci, promoted CD25highFoxp3+ Treg differentiation. An increased Treg induction was observed when cysticerci were present. Moreover, an augmentation of suppressive-related molecules (SLAMF1, B7-H1, and CD205) was found in parasite-induced DC differentiation. Increased Tregs and a higher in vivo DC expression of the regulatory molecules SLAMF1 and CD205 in NC patients were also found. SLAMF1 gene was downregulated in NC patients with extraparenchymal cysticerci, exhibiting higher inflammation levels than patients with parenchymal parasites. Our findings suggest that cysticerci may modulate DC to favor a suppressive environment, which may help parasite establishment, minimizing the excessive inflammation, which may lead to tissue damage.http://dx.doi.org/10.1155/2013/981468 |
| spellingShingle | Laura Adalid-Peralta Asiel Arce-Sillas Gladis Fragoso Graciela Cárdenas Marcos Rosetti Didier Casanova-Hernández Claudia Rangel-Escareño Laura Uribe-Figueroa Agnes Fleury Edda Sciutto Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation Clinical and Developmental Immunology |
| title | Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation |
| title_full | Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation |
| title_fullStr | Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation |
| title_full_unstemmed | Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation |
| title_short | Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation |
| title_sort | cysticerci drive dendritic cells to promote in vitro and in vivo tregs differentiation |
| url | http://dx.doi.org/10.1155/2013/981468 |
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