Increased NTPDase Activity in Lymphocytes during Experimental Sepsis
We investigated in rats induced to sepsis the activity of ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39; E.C. 3.6.1.5), an enzyme involved in the modulation of immune responses. After 12 hours of surgery, lymphocytes were isolated from blood and NTPDase activity was determined. It wa...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/941906 |
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| author | Claudia de Mello Bertoncheli Carine Eloise Prestes Zimmermann Jeandre Augusto dos Santos Jaques Cláudio Alberto Martins Leal Jader Betsch Ruchel Bruna Cipolatto Rocha Kelly de Vargas Pinheiro Viviane do Carmo Gonçalves Souza Daniel Roulim Stainki Sônia Cristina Almeida Luz Maria Rosa Chitolina Schetinger Daniela Bitencourt Rosa Leal |
| author_facet | Claudia de Mello Bertoncheli Carine Eloise Prestes Zimmermann Jeandre Augusto dos Santos Jaques Cláudio Alberto Martins Leal Jader Betsch Ruchel Bruna Cipolatto Rocha Kelly de Vargas Pinheiro Viviane do Carmo Gonçalves Souza Daniel Roulim Stainki Sônia Cristina Almeida Luz Maria Rosa Chitolina Schetinger Daniela Bitencourt Rosa Leal |
| author_sort | Claudia de Mello Bertoncheli |
| collection | DOAJ |
| description | We investigated in rats induced to sepsis the activity of ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39; E.C. 3.6.1.5), an enzyme involved in the modulation of immune responses. After 12 hours of surgery, lymphocytes were isolated from blood and NTPDase activity was determined. It was also performed the histology of kidney, liver, and lung. The results demonstrated an increase in the hydrolysis of adenosine-5′-triphosphate (ATP) (P<0.01), but no changes regarding adenosine-5′-monophosphate (ADP) hydrolysis (P>0.05). Histological analysis showed several morphological changes in the septic group, such as vascular congestion, necrosis, and infiltration of mononuclear cells. It is known that the intracellular milieu contains much more ATP nucleotides than the extracellular. In this context, the increased ATPasic activity was probably induced as a dynamic response to clean up the elevated ATP levels resulting from cellular death. |
| format | Article |
| id | doaj-art-645f6f91b893464bb36f19eed2b959e9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-645f6f91b893464bb36f19eed2b959e92025-02-03T05:43:36ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/941906941906Increased NTPDase Activity in Lymphocytes during Experimental SepsisClaudia de Mello Bertoncheli0Carine Eloise Prestes Zimmermann1Jeandre Augusto dos Santos Jaques2Cláudio Alberto Martins Leal3Jader Betsch Ruchel4Bruna Cipolatto Rocha5Kelly de Vargas Pinheiro6Viviane do Carmo Gonçalves Souza7Daniel Roulim Stainki8Sônia Cristina Almeida Luz9Maria Rosa Chitolina Schetinger10Daniela Bitencourt Rosa Leal11Centro de Ciências da Saúde, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilCentro de Ciências da Saúde, Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, BrazilWe investigated in rats induced to sepsis the activity of ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39; E.C. 3.6.1.5), an enzyme involved in the modulation of immune responses. After 12 hours of surgery, lymphocytes were isolated from blood and NTPDase activity was determined. It was also performed the histology of kidney, liver, and lung. The results demonstrated an increase in the hydrolysis of adenosine-5′-triphosphate (ATP) (P<0.01), but no changes regarding adenosine-5′-monophosphate (ADP) hydrolysis (P>0.05). Histological analysis showed several morphological changes in the septic group, such as vascular congestion, necrosis, and infiltration of mononuclear cells. It is known that the intracellular milieu contains much more ATP nucleotides than the extracellular. In this context, the increased ATPasic activity was probably induced as a dynamic response to clean up the elevated ATP levels resulting from cellular death.http://dx.doi.org/10.1100/2012/941906 |
| spellingShingle | Claudia de Mello Bertoncheli Carine Eloise Prestes Zimmermann Jeandre Augusto dos Santos Jaques Cláudio Alberto Martins Leal Jader Betsch Ruchel Bruna Cipolatto Rocha Kelly de Vargas Pinheiro Viviane do Carmo Gonçalves Souza Daniel Roulim Stainki Sônia Cristina Almeida Luz Maria Rosa Chitolina Schetinger Daniela Bitencourt Rosa Leal Increased NTPDase Activity in Lymphocytes during Experimental Sepsis The Scientific World Journal |
| title | Increased NTPDase Activity in Lymphocytes during Experimental Sepsis |
| title_full | Increased NTPDase Activity in Lymphocytes during Experimental Sepsis |
| title_fullStr | Increased NTPDase Activity in Lymphocytes during Experimental Sepsis |
| title_full_unstemmed | Increased NTPDase Activity in Lymphocytes during Experimental Sepsis |
| title_short | Increased NTPDase Activity in Lymphocytes during Experimental Sepsis |
| title_sort | increased ntpdase activity in lymphocytes during experimental sepsis |
| url | http://dx.doi.org/10.1100/2012/941906 |
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