Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells
The aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism. MIN6 cells were, respectively, treated with 0 μM, 2 μM, 5 μM, and 10 μM simvastatin for 48 h. Radio immunoassay was performed to measure the effect of simvastatin...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/376570 |
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| author | Jieqiong Zhou Weihua Li Qiang Xie Yuxi Hou Shaopeng Zhan Xi Yang Xiaofeng Xu Jun Cai Zhengrong Huang |
| author_facet | Jieqiong Zhou Weihua Li Qiang Xie Yuxi Hou Shaopeng Zhan Xi Yang Xiaofeng Xu Jun Cai Zhengrong Huang |
| author_sort | Jieqiong Zhou |
| collection | DOAJ |
| description | The aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism. MIN6 cells were, respectively, treated with 0 μM, 2 μM, 5 μM, and 10 μM simvastatin for 48 h. Radio immunoassay was performed to measure the effect of simvastatin on insulin secretion in MIN6 cells. Luciferase method was used to examine the content of ATP in MIN6 cells. Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 (Cav1.2), and glucose transporter-2 (GLUT2), respectively. ATP-sensitive potassium current and L-type calcium current were recorded by whole-cell patch-clamp technique. The results showed that high concentrations of simvastatin (5 μM and 10 μM) significantly reduced the synthesis and secretion of insulin compared to control groups in MIN6 cells (P<0.05). ATP content in simvastatin-treated cells was lower than in control cells (P<0.05). Compared with control group, the mRNA and protein expression of Kir6.2 increased with treatment of simvastatin (P<0.05), and mRNA and protein expression of Cav1.2 and GLUT2 decreased in response to simvastatin (P<0.05). Moreover, simvastatin increased the ATP-sensitive potassium current and reduced the L-type calcium current. These results suggest that simvastatin inhibits the synthesis and secretion of insulin through a reduction in saccharometabolism in MIN6 cells. |
| format | Article |
| id | doaj-art-645e03cae8204f76bc0a9f589bf9efa1 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-645e03cae8204f76bc0a9f589bf9efa12025-02-03T06:07:02ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/376570376570Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 CellsJieqiong Zhou0Weihua Li1Qiang Xie2Yuxi Hou3Shaopeng Zhan4Xi Yang5Xiaofeng Xu6Jun Cai7Zhengrong Huang8Department of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaDepartment of Cardiology, Chaoyang Hospital, Capital Medical University, 8th Gongtinanlu Road, Chaoyang District, Beijing 100020, ChinaDepartment of Cardiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Xiamen 361003, ChinaThe aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism. MIN6 cells were, respectively, treated with 0 μM, 2 μM, 5 μM, and 10 μM simvastatin for 48 h. Radio immunoassay was performed to measure the effect of simvastatin on insulin secretion in MIN6 cells. Luciferase method was used to examine the content of ATP in MIN6 cells. Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 (Cav1.2), and glucose transporter-2 (GLUT2), respectively. ATP-sensitive potassium current and L-type calcium current were recorded by whole-cell patch-clamp technique. The results showed that high concentrations of simvastatin (5 μM and 10 μM) significantly reduced the synthesis and secretion of insulin compared to control groups in MIN6 cells (P<0.05). ATP content in simvastatin-treated cells was lower than in control cells (P<0.05). Compared with control group, the mRNA and protein expression of Kir6.2 increased with treatment of simvastatin (P<0.05), and mRNA and protein expression of Cav1.2 and GLUT2 decreased in response to simvastatin (P<0.05). Moreover, simvastatin increased the ATP-sensitive potassium current and reduced the L-type calcium current. These results suggest that simvastatin inhibits the synthesis and secretion of insulin through a reduction in saccharometabolism in MIN6 cells.http://dx.doi.org/10.1155/2014/376570 |
| spellingShingle | Jieqiong Zhou Weihua Li Qiang Xie Yuxi Hou Shaopeng Zhan Xi Yang Xiaofeng Xu Jun Cai Zhengrong Huang Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells Journal of Diabetes Research |
| title | Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells |
| title_full | Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells |
| title_fullStr | Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells |
| title_full_unstemmed | Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells |
| title_short | Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells |
| title_sort | effects of simvastatin on glucose metabolism in mouse min6 cells |
| url | http://dx.doi.org/10.1155/2014/376570 |
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