Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats
Background. Omega-3 (ω3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects of ω3, in a model of global ischemia. Methods. Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups wer...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2016/6462120 |
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| author | Maria Elizabeth Pereira Nobre Alyne Oliveira Correia Francisco Nilson Maciel Mendonça Luiz Ricardo Araújo Uchoa Jessica Tamara Nunes Vasconcelos Carlos Ney Alencar de Araújo Gerly Anne de Castro Brito Rafaelly Maria Pinheiro Siqueira Gilberto dos Santos Cerqueira Kelly Rose Tavares Neves Ricardo Mário Arida Glauce Socorro de Barros Viana |
| author_facet | Maria Elizabeth Pereira Nobre Alyne Oliveira Correia Francisco Nilson Maciel Mendonça Luiz Ricardo Araújo Uchoa Jessica Tamara Nunes Vasconcelos Carlos Ney Alencar de Araújo Gerly Anne de Castro Brito Rafaelly Maria Pinheiro Siqueira Gilberto dos Santos Cerqueira Kelly Rose Tavares Neves Ricardo Mário Arida Glauce Socorro de Barros Viana |
| author_sort | Maria Elizabeth Pereira Nobre |
| collection | DOAJ |
| description | Background. Omega-3 (ω3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects of ω3, in a model of global ischemia. Methods. Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups were SO, untreated ischemic and ischemic treated rats with ω3 (5 and 10 mg/kg, 7 days). The SO and untreated ischemic animals were orally treated with 1% cremophor and, 1 h after the last administration, they were behaviorally tested and euthanized for neurochemical (DA, DOPAC, and NE determinations), histological (Fluoro jade staining), and immunohistochemical (TNF-alpha, COX-2 and iNOS) evaluations. The data were analyzed by ANOVA and Newman-Keuls as the post hoc test. Results. Ischemia increased the locomotor activity and rearing behavior that were partly reversed by ω3. Ischemia decreased striatal DA and DOPAC contents and increased NE contents, effects reversed by ω3. This drug protected hippocampal neuron degeneration, as observed by Fluoro-Jade staining, and the increased immunostainings for TNF-alpha, COX-2, and iNOS were partly or totally blocked by ω3. Conclusion. This study showed a neuroprotective effect of ω3, in great part due to its anti-inflammatory properties, stimulating translational studies focusing on its use in clinic for stroke managing. |
| format | Article |
| id | doaj-art-64598935c3ac4d2a9835d5bb015dfcf5 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-64598935c3ac4d2a9835d5bb015dfcf52025-08-20T03:34:24ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322016-01-01201610.1155/2016/64621206462120Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in RatsMaria Elizabeth Pereira Nobre0Alyne Oliveira Correia1Francisco Nilson Maciel Mendonça2Luiz Ricardo Araújo Uchoa3Jessica Tamara Nunes Vasconcelos4Carlos Ney Alencar de Araújo5Gerly Anne de Castro Brito6Rafaelly Maria Pinheiro Siqueira7Gilberto dos Santos Cerqueira8Kelly Rose Tavares Neves9Ricardo Mário Arida10Glauce Socorro de Barros Viana11Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilFederal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, BrazilFederal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, BrazilFederal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, BrazilFederal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, BrazilFederal University of São Paulo (UNIFESP), Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, BrazilFaculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, BrazilBackground. Omega-3 (ω3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects of ω3, in a model of global ischemia. Methods. Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups were SO, untreated ischemic and ischemic treated rats with ω3 (5 and 10 mg/kg, 7 days). The SO and untreated ischemic animals were orally treated with 1% cremophor and, 1 h after the last administration, they were behaviorally tested and euthanized for neurochemical (DA, DOPAC, and NE determinations), histological (Fluoro jade staining), and immunohistochemical (TNF-alpha, COX-2 and iNOS) evaluations. The data were analyzed by ANOVA and Newman-Keuls as the post hoc test. Results. Ischemia increased the locomotor activity and rearing behavior that were partly reversed by ω3. Ischemia decreased striatal DA and DOPAC contents and increased NE contents, effects reversed by ω3. This drug protected hippocampal neuron degeneration, as observed by Fluoro-Jade staining, and the increased immunostainings for TNF-alpha, COX-2, and iNOS were partly or totally blocked by ω3. Conclusion. This study showed a neuroprotective effect of ω3, in great part due to its anti-inflammatory properties, stimulating translational studies focusing on its use in clinic for stroke managing.http://dx.doi.org/10.1155/2016/6462120 |
| spellingShingle | Maria Elizabeth Pereira Nobre Alyne Oliveira Correia Francisco Nilson Maciel Mendonça Luiz Ricardo Araújo Uchoa Jessica Tamara Nunes Vasconcelos Carlos Ney Alencar de Araújo Gerly Anne de Castro Brito Rafaelly Maria Pinheiro Siqueira Gilberto dos Santos Cerqueira Kelly Rose Tavares Neves Ricardo Mário Arida Glauce Socorro de Barros Viana Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats Journal of Nutrition and Metabolism |
| title | Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats |
| title_full | Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats |
| title_fullStr | Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats |
| title_full_unstemmed | Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats |
| title_short | Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats |
| title_sort | omega 3 fatty acids possible neuroprotective mechanisms in the model of global ischemia in rats |
| url | http://dx.doi.org/10.1155/2016/6462120 |
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