Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines
Galectins are found in the placenta during both normal and pathological conditions. Specifically, galectins -1, -3, and -8 have been identified in extravillous trophoblast cells, with galectins -1, and -3 playing notable roles in trophoblast cell function. Altered expression profiles of galectins ar...
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University of Belgrade, University of Novi Sad
2024-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400022J.pdf |
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| author | Jovanović Krivokuća Milica Legner Janko Ćujić Danica Vilotić Aleksandra Grubiša Ivana Vićovac Ljiljana Bojić-Trbojević Žanka |
| author_facet | Jovanović Krivokuća Milica Legner Janko Ćujić Danica Vilotić Aleksandra Grubiša Ivana Vićovac Ljiljana Bojić-Trbojević Žanka |
| author_sort | Jovanović Krivokuća Milica |
| collection | DOAJ |
| description | Galectins are found in the placenta during both normal and pathological conditions. Specifically, galectins -1, -3, and -8 have been identified in extravillous trophoblast cells, with galectins -1, and -3 playing notable roles in trophoblast cell function. Altered expression profiles of galectins are linked to various cancers. This study explores the expression and localization of galectins -1, -3, and -8 in a normal extravillous HTR-8/SVneo cell line and two choriocarcinoma cell lines, JAR and JEG-3. Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. Galectins -1, -3, and -8 were expressed at lower levels in JAR and JEG-3 cells compared to HTR-8/SVneo cells. Additionally, different splice variants of galectin-8 were detected across all cell lines. Galectin-1 was predominantly localized at the plasma membrane. Notable differences in galectin-3 localization and distribution were observed between HTR-8/SVneo cells and choriocarcinoma cells. These findings highlight variations in the expression, staining patterns, and subcellular distribution of galectins -1, -3, and -8, suggesting their potential involvement in the pathology of choriocarcinoma. |
| format | Article |
| id | doaj-art-643e6e9b1a1a49c08d9d132e00f0d356 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-643e6e9b1a1a49c08d9d132e00f0d3562025-01-30T06:38:33ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176330331210.2298/ABS240605022J0354-46642400022JComparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell linesJovanović Krivokuća Milica0https://orcid.org/0000-0001-8292-4853Legner Janko1https://orcid.org/0000-0003-3720-1933Ćujić Danica2https://orcid.org/0000-0002-6145-3850Vilotić Aleksandra3https://orcid.org/0000-0002-0898-5401Grubiša Ivana4https://orcid.org/0000-0001-8352-0383Vićovac Ljiljana5https://orcid.org/0000-0002-2744-415XBojić-Trbojević Žanka6https://orcid.org/0000-0001-5619-1027Department for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaDepartment for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaDepartment for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaDepartment for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaDepartment of Human Genetics, Zvezdara University Medical Center, University of Belgrade, Belgrade, SerbiaDepartment for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaDepartment for the Biology of Reproduction, Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Belgrade, SerbiaGalectins are found in the placenta during both normal and pathological conditions. Specifically, galectins -1, -3, and -8 have been identified in extravillous trophoblast cells, with galectins -1, and -3 playing notable roles in trophoblast cell function. Altered expression profiles of galectins are linked to various cancers. This study explores the expression and localization of galectins -1, -3, and -8 in a normal extravillous HTR-8/SVneo cell line and two choriocarcinoma cell lines, JAR and JEG-3. Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. Galectins -1, -3, and -8 were expressed at lower levels in JAR and JEG-3 cells compared to HTR-8/SVneo cells. Additionally, different splice variants of galectin-8 were detected across all cell lines. Galectin-1 was predominantly localized at the plasma membrane. Notable differences in galectin-3 localization and distribution were observed between HTR-8/SVneo cells and choriocarcinoma cells. These findings highlight variations in the expression, staining patterns, and subcellular distribution of galectins -1, -3, and -8, suggesting their potential involvement in the pathology of choriocarcinoma.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400022J.pdfgalectin-1galectin-3galectin-8extravillous trophoblastchoriocarcinoma cells |
| spellingShingle | Jovanović Krivokuća Milica Legner Janko Ćujić Danica Vilotić Aleksandra Grubiša Ivana Vićovac Ljiljana Bojić-Trbojević Žanka Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines Archives of Biological Sciences galectin-1 galectin-3 galectin-8 extravillous trophoblast choriocarcinoma cells |
| title | Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines |
| title_full | Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines |
| title_fullStr | Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines |
| title_full_unstemmed | Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines |
| title_short | Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines |
| title_sort | comparative analysis of galectin 1 galectin 3 and galectin 8 signatures in normal and choriocarcinoma trophoblast cell lines |
| topic | galectin-1 galectin-3 galectin-8 extravillous trophoblast choriocarcinoma cells |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400022J.pdf |
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