Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis
Chronic apical periodontitis is characterized by alveolar bone absorption in the apical region and is the result of the participation of various inflammatory mediators. Studies have shown that the Bruton tyrosine kinase- (Btk-) phospholipase Cγ2 (PLCγ2) signaling pathway plays an important role in b...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/8767529 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564823919951872 |
|---|---|
| author | Lina Wang Hong Zhang Ming Dong Meina Zuo Shuo Liu Ying Lu Weidong Niu |
| author_facet | Lina Wang Hong Zhang Ming Dong Meina Zuo Shuo Liu Ying Lu Weidong Niu |
| author_sort | Lina Wang |
| collection | DOAJ |
| description | Chronic apical periodontitis is characterized by alveolar bone absorption in the apical region and is the result of the participation of various inflammatory mediators. Studies have shown that the Bruton tyrosine kinase- (Btk-) phospholipase Cγ2 (PLCγ2) signaling pathway plays an important role in bone absorption, but it is unknown whether it plays a role in apical periodontitis bone destruction. Therefore, this study verified the role of Btk and PLCγ2 in bone resorption of apical periodontitis by in vivo and in vitro experiments. In the in vivo experiment, a mice model of apical periodontitis was established; apical bone resorption was confirmed by the numbers of osteoclasts and HE staining. Btk, PLCγ2, and nuclear factor of activated T-cells 1 (NFATc-1) were detected by immunohistochemical staining. In the in vitro experiment, lipopolysaccharides (LPS) were used to stimulate osteoclast precursor cell RAW264.7 to establish an inflammatory microenvironment and detect osteoclast differentiation. By silencing Btk, the expression of Btk, PLCγ2, and NFATc-1 was detected by real-time qPCR and Western blot, and osteoclastogenesis was detected by enzyme histochemical staining to further confirm the role of Btk in bone resorption. It was found that the expression of Btk, PLCγ2, and NFATc-1 changed significantly with the progression of inflammation and bone destruction, indicating that Btk and PLCγ2 may be involved in the progression of inflammation in apical periodontitis and bone absorption. In vitro experiments confirmed that the differentiation of osteoclasts and the expression of PLCγ2 and NFATc-1 were significantly inhibited after silencing Btk expression, but osteoclast precursor cells could be differentiated due to the proinflammatory factor lipopolysaccharide. This study demonstrates that Btk and PLCγ2 are key factors involved in the apical inflammatory response and bone destruction. |
| format | Article |
| id | doaj-art-6437eacb12ee40c7bbd434a8ef9076fb |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6437eacb12ee40c7bbd434a8ef9076fb2025-02-03T01:10:09ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/87675298767529Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical PeriodontitisLina Wang0Hong Zhang1Ming Dong2Meina Zuo3Shuo Liu4Ying Lu5Weidong Niu6Department of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaDepartment of Endodontics and Periodontics, College of Stomatology, Dalian Medical University, Dalian, Liaoning Province, ChinaChronic apical periodontitis is characterized by alveolar bone absorption in the apical region and is the result of the participation of various inflammatory mediators. Studies have shown that the Bruton tyrosine kinase- (Btk-) phospholipase Cγ2 (PLCγ2) signaling pathway plays an important role in bone absorption, but it is unknown whether it plays a role in apical periodontitis bone destruction. Therefore, this study verified the role of Btk and PLCγ2 in bone resorption of apical periodontitis by in vivo and in vitro experiments. In the in vivo experiment, a mice model of apical periodontitis was established; apical bone resorption was confirmed by the numbers of osteoclasts and HE staining. Btk, PLCγ2, and nuclear factor of activated T-cells 1 (NFATc-1) were detected by immunohistochemical staining. In the in vitro experiment, lipopolysaccharides (LPS) were used to stimulate osteoclast precursor cell RAW264.7 to establish an inflammatory microenvironment and detect osteoclast differentiation. By silencing Btk, the expression of Btk, PLCγ2, and NFATc-1 was detected by real-time qPCR and Western blot, and osteoclastogenesis was detected by enzyme histochemical staining to further confirm the role of Btk in bone resorption. It was found that the expression of Btk, PLCγ2, and NFATc-1 changed significantly with the progression of inflammation and bone destruction, indicating that Btk and PLCγ2 may be involved in the progression of inflammation in apical periodontitis and bone absorption. In vitro experiments confirmed that the differentiation of osteoclasts and the expression of PLCγ2 and NFATc-1 were significantly inhibited after silencing Btk expression, but osteoclast precursor cells could be differentiated due to the proinflammatory factor lipopolysaccharide. This study demonstrates that Btk and PLCγ2 are key factors involved in the apical inflammatory response and bone destruction.http://dx.doi.org/10.1155/2019/8767529 |
| spellingShingle | Lina Wang Hong Zhang Ming Dong Meina Zuo Shuo Liu Ying Lu Weidong Niu Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis Mediators of Inflammation |
| title | Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis |
| title_full | Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis |
| title_fullStr | Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis |
| title_full_unstemmed | Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis |
| title_short | Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis |
| title_sort | role of the btk plcγ2 signaling pathway in the bone destruction of apical periodontitis |
| url | http://dx.doi.org/10.1155/2019/8767529 |
| work_keys_str_mv | AT linawang roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT hongzhang roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT mingdong roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT meinazuo roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT shuoliu roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT yinglu roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis AT weidongniu roleofthebtkplcg2signalingpathwayinthebonedestructionofapicalperiodontitis |