Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone
Smartphones are widely spread and their usage does not require any trained personnel. Recently, smartphones were successfully used in analytical chemistry as a simple detection tool in some applications. This paper focuses on immobilization of acetylcholinesterase (AChE) onto commercially available...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/3712384 |
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| _version_ | 1832558727879720960 |
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| author | Adam Kostelnik Alexander Cegan Miroslav Pohanka |
| author_facet | Adam Kostelnik Alexander Cegan Miroslav Pohanka |
| author_sort | Adam Kostelnik |
| collection | DOAJ |
| description | Smartphones are widely spread and their usage does not require any trained personnel. Recently, smartphones were successfully used in analytical chemistry as a simple detection tool in some applications. This paper focuses on immobilization of acetylcholinesterase (AChE) onto commercially available pH strips with stabilization in the gelatin membrane. AChE degrades acetylcholine into choline and acetic acid which causes color change of acid-base indicator. Smartphone served as a tool for measurement of indicator color change from red to orange while inhibitors blocked this process. AChE inhibitors were measured with limits of detection, 149 nM and 22.3 nM for galanthamine and donepezil, respectively. Organic solvents were measured for method interferences. Measurement procedure was performed on 3D printed holder and digital photography was evaluated using red-green-blue (RGB) channels. The invented assay was validated to the standard Ellman’s test and verified on murine plasma samples spiked with inhibitors. We consider that the assay is fully suitable for practical performance. |
| format | Article |
| id | doaj-art-642c0fcf98db4395a4257850d86043ee |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-642c0fcf98db4395a4257850d86043ee2025-02-03T01:31:46ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792017-01-01201710.1155/2017/37123843712384Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a SmartphoneAdam Kostelnik0Alexander Cegan1Miroslav Pohanka2Faculty of Chemical Technology, University of Pardubice, Studentska 95, 53210 Pardubice, Czech RepublicFaculty of Chemical Technology, University of Pardubice, Studentska 95, 53210 Pardubice, Czech RepublicFaculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech RepublicSmartphones are widely spread and their usage does not require any trained personnel. Recently, smartphones were successfully used in analytical chemistry as a simple detection tool in some applications. This paper focuses on immobilization of acetylcholinesterase (AChE) onto commercially available pH strips with stabilization in the gelatin membrane. AChE degrades acetylcholine into choline and acetic acid which causes color change of acid-base indicator. Smartphone served as a tool for measurement of indicator color change from red to orange while inhibitors blocked this process. AChE inhibitors were measured with limits of detection, 149 nM and 22.3 nM for galanthamine and donepezil, respectively. Organic solvents were measured for method interferences. Measurement procedure was performed on 3D printed holder and digital photography was evaluated using red-green-blue (RGB) channels. The invented assay was validated to the standard Ellman’s test and verified on murine plasma samples spiked with inhibitors. We consider that the assay is fully suitable for practical performance.http://dx.doi.org/10.1155/2017/3712384 |
| spellingShingle | Adam Kostelnik Alexander Cegan Miroslav Pohanka Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone International Journal of Analytical Chemistry |
| title | Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone |
| title_full | Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone |
| title_fullStr | Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone |
| title_full_unstemmed | Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone |
| title_short | Acetylcholinesterase Inhibitors Assay Using Colorimetric pH Sensitive Strips and Image Analysis by a Smartphone |
| title_sort | acetylcholinesterase inhibitors assay using colorimetric ph sensitive strips and image analysis by a smartphone |
| url | http://dx.doi.org/10.1155/2017/3712384 |
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