A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics
A preferable approach of a combination of a multiwavelength fusion HPLC fingerprint and chemometrics for the quality control of Xiaoer Chiqiao Qingre granules (XCQG) was established in this study. A single-wavelength HPLC fingerprint was performed to identify 18 peaks as common peaks in the beginnin...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/6836981 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546878507450368 |
|---|---|
| author | Tong Xu Xiaoqi Li Mengmeng Huang Qi Wang Chao Li Gang Tian Yan Chen |
| author_facet | Tong Xu Xiaoqi Li Mengmeng Huang Qi Wang Chao Li Gang Tian Yan Chen |
| author_sort | Tong Xu |
| collection | DOAJ |
| description | A preferable approach of a combination of a multiwavelength fusion HPLC fingerprint and chemometrics for the quality control of Xiaoer Chiqiao Qingre granules (XCQG) was established in this study. A single-wavelength HPLC fingerprint was performed to identify 18 peaks as common peaks in the beginning, and 12 of them were recognized by HPLC-Q/TOF-MS. To overcome the limitation of the single-wavelength HPLC fingerprint, a three-wavelength (230 nm, 250 nm, and 330 nm) fusion fingerprint was established for a more thorough quality assessment. Six main active ingredients (geniposide, paeoniflorin, forsythin, forsythoside A, baicalin, and wogonoside) were selected as chemical markers for simultaneous quantitative analysis, while the results indicated that the content of other five ingredients except forsythoside A presented comparatively stable. Chemometrics including hierarchical cluster analysis (HCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to evaluate the homogeneity and heterogeneity of sixteen batches of XCQG. The results of the multiwavelength fingerprint were clearly classified into two clusters by HCA, whereas the single-wavelength fingerprint showed no distinct difference between them. OPLS-DA was further employed to prove that the above six main active ingredients made great contributions to clustering. In summary, this integrated analysis provided a better promoted and more comprehensive method to control the quality of XCQG. |
| format | Article |
| id | doaj-art-64151b892bb74167b29a8144ce4d2b64 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-64151b892bb74167b29a8144ce4d2b642025-02-03T06:46:41ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/68369816836981A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and ChemometricsTong Xu0Xiaoqi Li1Mengmeng Huang2Qi Wang3Chao Li4Gang Tian5Yan Chen6Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, ChinaJumpcan Pharmaceutical Co., Ltd., Taixing 225400, ChinaJumpcan Pharmaceutical Co., Ltd., Taixing 225400, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, ChinaA preferable approach of a combination of a multiwavelength fusion HPLC fingerprint and chemometrics for the quality control of Xiaoer Chiqiao Qingre granules (XCQG) was established in this study. A single-wavelength HPLC fingerprint was performed to identify 18 peaks as common peaks in the beginning, and 12 of them were recognized by HPLC-Q/TOF-MS. To overcome the limitation of the single-wavelength HPLC fingerprint, a three-wavelength (230 nm, 250 nm, and 330 nm) fusion fingerprint was established for a more thorough quality assessment. Six main active ingredients (geniposide, paeoniflorin, forsythin, forsythoside A, baicalin, and wogonoside) were selected as chemical markers for simultaneous quantitative analysis, while the results indicated that the content of other five ingredients except forsythoside A presented comparatively stable. Chemometrics including hierarchical cluster analysis (HCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to evaluate the homogeneity and heterogeneity of sixteen batches of XCQG. The results of the multiwavelength fingerprint were clearly classified into two clusters by HCA, whereas the single-wavelength fingerprint showed no distinct difference between them. OPLS-DA was further employed to prove that the above six main active ingredients made great contributions to clustering. In summary, this integrated analysis provided a better promoted and more comprehensive method to control the quality of XCQG.http://dx.doi.org/10.1155/2020/6836981 |
| spellingShingle | Tong Xu Xiaoqi Li Mengmeng Huang Qi Wang Chao Li Gang Tian Yan Chen A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics Journal of Analytical Methods in Chemistry |
| title | A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics |
| title_full | A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics |
| title_fullStr | A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics |
| title_full_unstemmed | A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics |
| title_short | A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics |
| title_sort | preferable approach for the quality control of xiaoer chiqiao qingre granules based on the combination of chromatographic fingerprints and chemometrics |
| url | http://dx.doi.org/10.1155/2020/6836981 |
| work_keys_str_mv | AT tongxu apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT xiaoqili apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT mengmenghuang apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT qiwang apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT chaoli apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT gangtian apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT yanchen apreferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT tongxu preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT xiaoqili preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT mengmenghuang preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT qiwang preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT chaoli preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT gangtian preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics AT yanchen preferableapproachforthequalitycontrolofxiaoerchiqiaoqingregranulesbasedonthecombinationofchromatographicfingerprintsandchemometrics |