Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom
Introduction: Venomous snakes Bites are a serious public health issue, and in Mexico, Bothrops asper is responsible for the majority of viper bites. Objective: To evaluate, in a mouse model, the effect of Bothrops asper venom snake on liver enzymes LDH, AST, and ALT, as well as to assess its e...
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Universidad Autónoma del Estado México
2025-01-01
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| Series: | Revista de Medicina e Investigación Universidad Autónoma del Estado de México |
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| Online Access: | https://medicinainvestigacion.uaemex.mx/article/view/24277 |
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| author | Dr. Carlos Gallardo-Casas Ángel Gallardo-Casas María Teresa Valadez-Omaña Emmanuel Correa Solís María del Carmen Castillo-Hernández Rocio Ortiz-Butron |
| author_facet | Dr. Carlos Gallardo-Casas Ángel Gallardo-Casas María Teresa Valadez-Omaña Emmanuel Correa Solís María del Carmen Castillo-Hernández Rocio Ortiz-Butron |
| author_sort | Dr. Carlos Gallardo-Casas Ángel Gallardo-Casas |
| collection | DOAJ |
| description | Introduction: Venomous snakes Bites are a serious public health issue, and in Mexico, Bothrops asper is responsible for the majority of viper bites.
Objective: To evaluate, in a mouse model, the effect of Bothrops asper venom snake on liver enzymes LDH, AST, and ALT, as well as to assess its effect on renal and hepatic histology.
Methods: The LD50 was determined using a dose-response curve after intramuscular administration of increasing doses of the venom to mice (0.5, 1, 2.5, 5, and 10 mg of protein/kg). Subsequently, the mice were administered the LD25 value of the venom (or saline solution for control), and 24 hours after the injection, LDH, AST, and ALT enzymes were measured in the blood, and the liver and kidneys were collected from each animal.
Results: Results: Animals treated with poison (LD25 of 1 mg of protein/kg) had greater activity of AST, ALT and LDH enzymes, structural damage in the cortex and renal medulla, as well as in the liver after 24 hours of administration in the experimental group
Conclusion: B. asper venom causes an increase in LDH and transaminase enzymes, as well as renal and hepatic histopathological alterations. |
| format | Article |
| id | doaj-art-63f11318588844998c3a4ba164bd8f83 |
| institution | Kabale University |
| issn | 2594-0600 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidad Autónoma del Estado México |
| record_format | Article |
| series | Revista de Medicina e Investigación Universidad Autónoma del Estado de México |
| spelling | doaj-art-63f11318588844998c3a4ba164bd8f832025-01-23T16:45:02ZengUniversidad Autónoma del Estado MéxicoRevista de Medicina e Investigación Universidad Autónoma del Estado de México2594-06002025-01-01131111116https://doi.org/10.36677/medicinainvestigacion.v13i1.24277Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venomDr. Carlos Gallardo-Casas Ángel Gallardo-Casas0https://orcid.org/0000-0002-3532-0149María Teresa Valadez-Omaña1https://orcid.org/0009-0004-3013-2503Emmanuel Correa Solís2https://orcid.org/0000-0001-9394-8703María del Carmen Castillo-Hernández3https://orcid.org/0000-0003-1192-6776Rocio Ortiz-Butron4https://orcid.org/0000-0001-8958-5233Universidad de Chalcatongo, SUNEO, Oaxaca. México.CECyT No. 6, IPN México.Universidad de la Cañada, SUNEO, Oaxaca. MéxicoEscuela Superior de Medicina, IPN, MéxicoENCB-IPNIntroduction: Venomous snakes Bites are a serious public health issue, and in Mexico, Bothrops asper is responsible for the majority of viper bites. Objective: To evaluate, in a mouse model, the effect of Bothrops asper venom snake on liver enzymes LDH, AST, and ALT, as well as to assess its effect on renal and hepatic histology. Methods: The LD50 was determined using a dose-response curve after intramuscular administration of increasing doses of the venom to mice (0.5, 1, 2.5, 5, and 10 mg of protein/kg). Subsequently, the mice were administered the LD25 value of the venom (or saline solution for control), and 24 hours after the injection, LDH, AST, and ALT enzymes were measured in the blood, and the liver and kidneys were collected from each animal. Results: Results: Animals treated with poison (LD25 of 1 mg of protein/kg) had greater activity of AST, ALT and LDH enzymes, structural damage in the cortex and renal medulla, as well as in the liver after 24 hours of administration in the experimental group Conclusion: B. asper venom causes an increase in LDH and transaminase enzymes, as well as renal and hepatic histopathological alterations. https://medicinainvestigacion.uaemex.mx/article/view/24277venenodañoplanta medicinalsalud pública |
| spellingShingle | Dr. Carlos Gallardo-Casas Ángel Gallardo-Casas María Teresa Valadez-Omaña Emmanuel Correa Solís María del Carmen Castillo-Hernández Rocio Ortiz-Butron Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom Revista de Medicina e Investigación Universidad Autónoma del Estado de México veneno daño planta medicinal salud pública |
| title | Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom |
| title_full | Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom |
| title_fullStr | Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom |
| title_full_unstemmed | Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom |
| title_short | Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom |
| title_sort | murine model evaluation of biochemical and tissue damage induced by bothrops asper nauyaca snake venom |
| topic | veneno daño planta medicinal salud pública |
| url | https://medicinainvestigacion.uaemex.mx/article/view/24277 |
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