GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER
It is believed that the heterochromatic locus flamenco, controlling transposition of retrotransposon/retrovirus gypsy in Drosophila melanogaster, is a source of Piwi-interacting RNA. Piwi is the primary component of the RNA interference machinery. Mutations in piwi and flamenco have the same phenoty...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/160 |
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| author | F. A. Urusov L. N. Nefedova A. R. Lavrenov N. I. Romanova A. I. Kim |
| author_facet | F. A. Urusov L. N. Nefedova A. R. Lavrenov N. I. Romanova A. I. Kim |
| author_sort | F. A. Urusov |
| collection | DOAJ |
| description | It is believed that the heterochromatic locus flamenco, controlling transposition of retrotransposon/retrovirus gypsy in Drosophila melanogaster, is a source of Piwi-interacting RNA. Piwi is the primary component of the RNA interference machinery. Mutations in piwi and flamenco have the same phenotype – an enhanced transcription and frequency of transposition of the retrotransposon/retrovirus gypsy. This paper discusses the results of the complementation test, which involves crossing strains MS and SS, mutant for the flamenco locus, with the piwi strain 3, heterozygous for a mutation in the piwi gene, followed by study of the transcription level of gypsy in the ovaries and testes of hybrids derived from these crosses. Genetic (hybridological) and molecular analysis of the interaction of the flamenco and piwi loci has been conducted. It has been revealed that transcription of gypsy is differently regulated in testis and ovaries of strains SS and MS, apparently owing to the different activity of piRNA clusters in these tissues. It has been also shown that the piwi and flamenco genes interact complementarily in testis tissue. |
| format | Article |
| id | doaj-art-63ea7a0003b742f98086ffd7b213cb62 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-63ea7a0003b742f98086ffd7b213cb622025-02-01T09:57:59ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01173381389144GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTERF. A. Urusov0L. N. Nefedova1A. R. Lavrenov2N. I. Romanova3A. I. Kim4M.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaM.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaM.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaM.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaM.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaIt is believed that the heterochromatic locus flamenco, controlling transposition of retrotransposon/retrovirus gypsy in Drosophila melanogaster, is a source of Piwi-interacting RNA. Piwi is the primary component of the RNA interference machinery. Mutations in piwi and flamenco have the same phenotype – an enhanced transcription and frequency of transposition of the retrotransposon/retrovirus gypsy. This paper discusses the results of the complementation test, which involves crossing strains MS and SS, mutant for the flamenco locus, with the piwi strain 3, heterozygous for a mutation in the piwi gene, followed by study of the transcription level of gypsy in the ovaries and testes of hybrids derived from these crosses. Genetic (hybridological) and molecular analysis of the interaction of the flamenco and piwi loci has been conducted. It has been revealed that transcription of gypsy is differently regulated in testis and ovaries of strains SS and MS, apparently owing to the different activity of piRNA clusters in these tissues. It has been also shown that the piwi and flamenco genes interact complementarily in testis tissue.https://vavilov.elpub.ru/jour/article/view/160drosophila melanogasterflamencopiwimolecular analysis |
| spellingShingle | F. A. Urusov L. N. Nefedova A. R. Lavrenov N. I. Romanova A. I. Kim GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER Вавиловский журнал генетики и селекции drosophila melanogaster flamenco piwi molecular analysis |
| title | GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER |
| title_full | GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER |
| title_fullStr | GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER |
| title_full_unstemmed | GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER |
| title_short | GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER |
| title_sort | genetic and molecular analysis of complementation of the flamenco and piwi loci in drosophila melanogaster |
| topic | drosophila melanogaster flamenco piwi molecular analysis |
| url | https://vavilov.elpub.ru/jour/article/view/160 |
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