The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation
Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated impla...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/305638 |
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| author | Humberto Osvaldo Schwartz-Filho Kostas Bougas Paulo G. Coelho Ying Xue Mariko Hayashi Rafael Silveira Faeda Rosemary Adriana Chiérici Marcantonio Daisuke Ono Fumio Kobayashi Kamal Mustafa Ann Wennerberg Ryo Jimbo |
| author_facet | Humberto Osvaldo Schwartz-Filho Kostas Bougas Paulo G. Coelho Ying Xue Mariko Hayashi Rafael Silveira Faeda Rosemary Adriana Chiérici Marcantonio Daisuke Ono Fumio Kobayashi Kamal Mustafa Ann Wennerberg Ryo Jimbo |
| author_sort | Humberto Osvaldo Schwartz-Filho |
| collection | DOAJ |
| description | Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface. |
| format | Article |
| id | doaj-art-63d1e50b77934a5bb36d014cc0bc1888 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-63d1e50b77934a5bb36d014cc0bc18882025-02-03T01:21:07ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/305638305638The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological EvaluationHumberto Osvaldo Schwartz-Filho0Kostas Bougas1Paulo G. Coelho2Ying Xue3Mariko Hayashi4Rafael Silveira Faeda5Rosemary Adriana Chiérici Marcantonio6Daisuke Ono7Fumio Kobayashi8Kamal Mustafa9Ann Wennerberg10Ryo Jimbo11Department of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Biomaterials and Biomimetics, New York University, New York, NY 10010, USADepartment of Clinical Dentistry, Center for Clinical Dental Research, University of Bergen, 5020 Bergen, NorwayDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDivision of Periodontology, Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP, São Paulo State University, 01049-010 Araraquara, SP, BrazilDivision of Periodontology, Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP, São Paulo State University, 01049-010 Araraquara, SP, BrazilDivision of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8102, JapanPrivate Practice, Kobe 658-0012, JapanDepartment of Clinical Dentistry, Center for Clinical Dental Research, University of Bergen, 5020 Bergen, NorwayDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenDepartment of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, SwedenAim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.http://dx.doi.org/10.1155/2012/305638 |
| spellingShingle | Humberto Osvaldo Schwartz-Filho Kostas Bougas Paulo G. Coelho Ying Xue Mariko Hayashi Rafael Silveira Faeda Rosemary Adriana Chiérici Marcantonio Daisuke Ono Fumio Kobayashi Kamal Mustafa Ann Wennerberg Ryo Jimbo The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation International Journal of Biomaterials |
| title | The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation |
| title_full | The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation |
| title_fullStr | The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation |
| title_full_unstemmed | The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation |
| title_short | The Effect of Laminin-1-Doped Nanoroughened Implant Surfaces: Gene Expression and Morphological Evaluation |
| title_sort | effect of laminin 1 doped nanoroughened implant surfaces gene expression and morphological evaluation |
| url | http://dx.doi.org/10.1155/2012/305638 |
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