Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients
Abstract Background Liver transplantation (LT) is a critical intervention for individuals with end-stage liver disease; yet, post-transplant problems, especially infections, graft rejection, and chronic liver disease, are often linked to systemic inflammation. Cytokines, small signaling molecules, s...
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BMC
2025-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-025-10466-9 |
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| author | Mohamed Abdel-Raoof Fouda Mohamed Abdel-Wahhab Ahmed Esmail Abdelkader Mohsen El-Sayd Ibrahim Taher Abozeid Elsheikh Hisham Mohammad Aldeweik Nora Elfeky |
| author_facet | Mohamed Abdel-Raoof Fouda Mohamed Abdel-Wahhab Ahmed Esmail Abdelkader Mohsen El-Sayd Ibrahim Taher Abozeid Elsheikh Hisham Mohammad Aldeweik Nora Elfeky |
| author_sort | Mohamed Abdel-Raoof Fouda |
| collection | DOAJ |
| description | Abstract Background Liver transplantation (LT) is a critical intervention for individuals with end-stage liver disease; yet, post-transplant problems, especially infections, graft rejection, and chronic liver disease, are often linked to systemic inflammation. Cytokines, small signaling molecules, significantly influence immune responses during and post-liver transplantation. Nonetheless, the intricate relationships among cytokines, immune responses, and the gut microbiota, especially gut dysbiosis, are still inadequately comprehended. Thus, this study aims to identify the gut microbiota (GM) and determine their relationship to cytokines (IL-17 and IL-10) in LT patients, due to their importance in enhancing the recovery rate. Result The research included 31 liver transplant (LT) patients from the Gastroenterology Surgical Center at Mansoura University, resulting in the collection of 174 stool and blood samples from all participants. Fourteen bacterial species have been identified in samples collected at three intervals: one week before, one week post, and two weeks post LT. A change in gut microbiota composition was noted, characterized by a rise in potentially pathogenic bacteria such as Enterococci and Enterobacteriaceae (including Escherichia coli and Klebsiella) and a reduction in beneficial bacteria such as Bacteroidetes and Firmicutes. The examination of patient demographic and clinical data revealed no significant correlations between sex, age, or diagnostic categories and gut microbiota composition. The findings of the Multivariate Analysis of Variance (MANOVA) indicated a substantial effect of gut microbiota composition on cytokine levels (IL-10 and IL-17), with all tests producing p-values of 0.001. The assessment of cytokine levels indicated fluctuating variations at several time points following surgery. IL-10 levels in the GM groups exhibited a statistically significant elevation during the second week post-surgery (p = 0.036), suggesting a potential recovery-related anti-inflammatory response. In contrast, IL-17 levels rose in the NI group over time, indicating a transition to a pro-inflammatory condition. Conclusion This study emphasizes the pivotal role of the gut microbiota in regulating immune responses following transplantation. |
| format | Article |
| id | doaj-art-63b1375ae66645f6843f78e4b6f7de1e |
| institution | Kabale University |
| issn | 1471-2334 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj-art-63b1375ae66645f6843f78e4b6f7de1e2025-02-02T12:10:32ZengBMCBMC Infectious Diseases1471-23342025-01-0125111010.1186/s12879-025-10466-9Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patientsMohamed Abdel-Raoof Fouda0Mohamed Abdel-Wahhab1Ahmed Esmail Abdelkader2Mohsen El-Sayd Ibrahim3Taher Abozeid Elsheikh4Hisham Mohammad Aldeweik5Nora Elfeky6Microbiology, Faculty of Medicine, Mansoura UniversityGeneral Surgery, Faculty of Medicine, Mansoura UniversityFaculty of Science, Port Said UniversityFaculty of Science, Port Said UniversityFaculty of Science, Port Said UniversityMicrobiology Lab., Environmental Monitoring Administration, Ministry of HealthBotany and Microbiology Department, Faculty of Science, Menoufia UniversityAbstract Background Liver transplantation (LT) is a critical intervention for individuals with end-stage liver disease; yet, post-transplant problems, especially infections, graft rejection, and chronic liver disease, are often linked to systemic inflammation. Cytokines, small signaling molecules, significantly influence immune responses during and post-liver transplantation. Nonetheless, the intricate relationships among cytokines, immune responses, and the gut microbiota, especially gut dysbiosis, are still inadequately comprehended. Thus, this study aims to identify the gut microbiota (GM) and determine their relationship to cytokines (IL-17 and IL-10) in LT patients, due to their importance in enhancing the recovery rate. Result The research included 31 liver transplant (LT) patients from the Gastroenterology Surgical Center at Mansoura University, resulting in the collection of 174 stool and blood samples from all participants. Fourteen bacterial species have been identified in samples collected at three intervals: one week before, one week post, and two weeks post LT. A change in gut microbiota composition was noted, characterized by a rise in potentially pathogenic bacteria such as Enterococci and Enterobacteriaceae (including Escherichia coli and Klebsiella) and a reduction in beneficial bacteria such as Bacteroidetes and Firmicutes. The examination of patient demographic and clinical data revealed no significant correlations between sex, age, or diagnostic categories and gut microbiota composition. The findings of the Multivariate Analysis of Variance (MANOVA) indicated a substantial effect of gut microbiota composition on cytokine levels (IL-10 and IL-17), with all tests producing p-values of 0.001. The assessment of cytokine levels indicated fluctuating variations at several time points following surgery. IL-10 levels in the GM groups exhibited a statistically significant elevation during the second week post-surgery (p = 0.036), suggesting a potential recovery-related anti-inflammatory response. In contrast, IL-17 levels rose in the NI group over time, indicating a transition to a pro-inflammatory condition. Conclusion This study emphasizes the pivotal role of the gut microbiota in regulating immune responses following transplantation.https://doi.org/10.1186/s12879-025-10466-9Liver transplantationGut microbiotaCytokineIL-10IL-17Nosocomial infection |
| spellingShingle | Mohamed Abdel-Raoof Fouda Mohamed Abdel-Wahhab Ahmed Esmail Abdelkader Mohsen El-Sayd Ibrahim Taher Abozeid Elsheikh Hisham Mohammad Aldeweik Nora Elfeky Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients BMC Infectious Diseases Liver transplantation Gut microbiota Cytokine IL-10 IL-17 Nosocomial infection |
| title | Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients |
| title_full | Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients |
| title_fullStr | Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients |
| title_full_unstemmed | Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients |
| title_short | Effect of gut microbiota changes on cytokines IL-10 and IL-17 levels in liver transplantation patients |
| title_sort | effect of gut microbiota changes on cytokines il 10 and il 17 levels in liver transplantation patients |
| topic | Liver transplantation Gut microbiota Cytokine IL-10 IL-17 Nosocomial infection |
| url | https://doi.org/10.1186/s12879-025-10466-9 |
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