Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties
The Compendium of Materia Medica highlights the therapeutic properties of <i>Trypoxylus dichotomus</i> (<i>T. dichotomus</i>). In this study, the species and content of volatile components, inorganic elements, and amino acids were measured, and the activity of crude extracts...
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2025-01-01
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| author | Dongyan Ye Qianhui Li Xuwen Liu Jie Zhou Shuren Yin Suyi Zhang Jing Wang Kh Ahammad Uz Zaman Helong Bai Fanlei Meng |
| author_facet | Dongyan Ye Qianhui Li Xuwen Liu Jie Zhou Shuren Yin Suyi Zhang Jing Wang Kh Ahammad Uz Zaman Helong Bai Fanlei Meng |
| author_sort | Dongyan Ye |
| collection | DOAJ |
| description | The Compendium of Materia Medica highlights the therapeutic properties of <i>Trypoxylus dichotomus</i> (<i>T. dichotomus</i>). In this study, the species and content of volatile components, inorganic elements, and amino acids were measured, and the activity of crude extracts of ethanol and water was studied. GC-MS analysis revealed 37–53 components across different life stages, excluding excessive heavy metals and containing essential trace elements. Amino acid profiling identified 15 types, rich in seven medicinal varieties. The in vitro anti-tumor testing of ethanol extracts from <i>T. dichotomus</i> adult males (TDA (M)) and <i>T. dichotomus</i> adult females (TDA (F)) was also performed. The experimental results showed that TDA (M) exhibited growth inhibition rates of 86.22% ± 1.19%, 85.42% ± 0.63%, 88.15% ± 3.27%, and 97.23% ± 0.60% against MKN-45, K-562, 239T, and 5637 tumor cells, respectively, while TDA (F) showed 81.28% ± 5.06% inhibition against K-562 cells. TDA best protected cells induced by H<sub>2</sub>O<sub>2</sub> at the concentration of 12.5 μg/mL, achieving a cell survival rate of 81%. Overall, TDA, TDA(F), and TDA(M) have notable anti-tumor and antioxidant activity, highlighting the significance of chemical analysis for their potential bioactivity. |
| format | Article |
| id | doaj-art-635cad6498014d75be6a97c956468346 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-635cad6498014d75be6a97c9564683462025-01-24T13:43:09ZengMDPI AGMolecules1420-30492025-01-0130222010.3390/molecules30020220Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective PropertiesDongyan Ye0Qianhui Li1Xuwen Liu2Jie Zhou3Shuren Yin4Suyi Zhang5Jing Wang6Kh Ahammad Uz Zaman7Helong Bai8Fanlei Meng9College of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaCollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaDKI College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, USACollege of Chemistry, Changchun Normal University, Changchun 130032, ChinaInstitute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, ChinaThe Compendium of Materia Medica highlights the therapeutic properties of <i>Trypoxylus dichotomus</i> (<i>T. dichotomus</i>). In this study, the species and content of volatile components, inorganic elements, and amino acids were measured, and the activity of crude extracts of ethanol and water was studied. GC-MS analysis revealed 37–53 components across different life stages, excluding excessive heavy metals and containing essential trace elements. Amino acid profiling identified 15 types, rich in seven medicinal varieties. The in vitro anti-tumor testing of ethanol extracts from <i>T. dichotomus</i> adult males (TDA (M)) and <i>T. dichotomus</i> adult females (TDA (F)) was also performed. The experimental results showed that TDA (M) exhibited growth inhibition rates of 86.22% ± 1.19%, 85.42% ± 0.63%, 88.15% ± 3.27%, and 97.23% ± 0.60% against MKN-45, K-562, 239T, and 5637 tumor cells, respectively, while TDA (F) showed 81.28% ± 5.06% inhibition against K-562 cells. TDA best protected cells induced by H<sub>2</sub>O<sub>2</sub> at the concentration of 12.5 μg/mL, achieving a cell survival rate of 81%. Overall, TDA, TDA(F), and TDA(M) have notable anti-tumor and antioxidant activity, highlighting the significance of chemical analysis for their potential bioactivity.https://www.mdpi.com/1420-3049/30/2/220<i>T. dichotomus</i>chemical componentsanti-tumorantioxidantneuro-antioxidant |
| spellingShingle | Dongyan Ye Qianhui Li Xuwen Liu Jie Zhou Shuren Yin Suyi Zhang Jing Wang Kh Ahammad Uz Zaman Helong Bai Fanlei Meng Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties Molecules <i>T. dichotomus</i> chemical components anti-tumor antioxidant neuro-antioxidant |
| title | Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties |
| title_full | Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties |
| title_fullStr | Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties |
| title_full_unstemmed | Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties |
| title_short | Comprehensive Analysis of <i>Trypoxylus dichotomus</i>: Inorganic Elements, Amino Acids, and Bioactive Compounds and Their Anticancer, Antioxidant, and Neuroprotective Properties |
| title_sort | comprehensive analysis of i trypoxylus dichotomus i inorganic elements amino acids and bioactive compounds and their anticancer antioxidant and neuroprotective properties |
| topic | <i>T. dichotomus</i> chemical components anti-tumor antioxidant neuro-antioxidant |
| url | https://www.mdpi.com/1420-3049/30/2/220 |
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