Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure
Objective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and five months were divided into four groups: CC (control dams, control offspring); DC (diabetic dams, control offspring); CFA (control dams, folic ac...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9151607 |
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| author | Carlos Donizete Pereira Júnior Camila Souza de Oliveira Guimarães Aline Cristina Souza da Silva Aldo Rogelis Aquiles Rodrigues Maria Aparecida da Glória Vicente de Paula Antunes Teixeira Niels Olsen Saraiva Câmara Lenaldo Branco Rocha Marlene Antônia dos Reis Juliana Reis Machado Laura Penna Rocha Fernanda Rodrigues Helmo Rosana Rosa Miranda Corrêa |
| author_facet | Carlos Donizete Pereira Júnior Camila Souza de Oliveira Guimarães Aline Cristina Souza da Silva Aldo Rogelis Aquiles Rodrigues Maria Aparecida da Glória Vicente de Paula Antunes Teixeira Niels Olsen Saraiva Câmara Lenaldo Branco Rocha Marlene Antônia dos Reis Juliana Reis Machado Laura Penna Rocha Fernanda Rodrigues Helmo Rosana Rosa Miranda Corrêa |
| author_sort | Carlos Donizete Pereira Júnior |
| collection | DOAJ |
| description | Objective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and five months were divided into four groups: CC (control dams, control offspring); DC (diabetic dams, control offspring); CFA (control dams, folic acid offspring, 250 mg/Kg); and DFA (diabetic dams, folic acid offspring). Gene expression of inflammatory markers MCP-1, IL-1, NOS3, TGF-β, TNF-α, and VEGF was evaluated by RT-PCR. Results. MCP-1 was increased in the CFA and DFA groups at two and five months of age, as well as in DC5 when compared to CC5. There was a higher expression of IL-1 in the CFA2, DFA2, and DC2 groups. There was a decrease in NOS3 and an increase in TNF-α in DFA5 in relation to CFA5. The gene expression of TGF-β increased in cases that had received folic acid at two and five months, and VEGF decreased in the CFA5 and DFA5 groups. DC5 showed increased VEGF expression in comparison with CC5. Conclusions. Gestational diabetes mellitus and folic acid both change the expression of inflammatory markers, thus demonstrating that the exposure to harmful agents in adulthood has a more severe impact in cases which underwent fetal reprogramming. |
| format | Article |
| id | doaj-art-63545c959e184416b36de8b012dbd764 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-63545c959e184416b36de8b012dbd7642025-02-03T01:12:38ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/91516079151607Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal FailureCarlos Donizete Pereira Júnior0Camila Souza de Oliveira Guimarães1Aline Cristina Souza da Silva2Aldo Rogelis Aquiles Rodrigues3Maria Aparecida da Glória4Vicente de Paula Antunes Teixeira5Niels Olsen Saraiva Câmara6Lenaldo Branco Rocha7Marlene Antônia dos Reis8Juliana Reis Machado9Laura Penna Rocha10Fernanda Rodrigues Helmo11Rosana Rosa Miranda Corrêa12Discipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDepartment of Health Sciences, Lavras Federal University, Lavras, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of Physiology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilNephrology Division, Federal University of São Paulo (UNIFESP), São Paulo, SP, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilNephrology Division, Federal University of São Paulo (UNIFESP), São Paulo, SP, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilDiscipline of General Pathology, Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba, MG, BrazilObjective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and five months were divided into four groups: CC (control dams, control offspring); DC (diabetic dams, control offspring); CFA (control dams, folic acid offspring, 250 mg/Kg); and DFA (diabetic dams, folic acid offspring). Gene expression of inflammatory markers MCP-1, IL-1, NOS3, TGF-β, TNF-α, and VEGF was evaluated by RT-PCR. Results. MCP-1 was increased in the CFA and DFA groups at two and five months of age, as well as in DC5 when compared to CC5. There was a higher expression of IL-1 in the CFA2, DFA2, and DC2 groups. There was a decrease in NOS3 and an increase in TNF-α in DFA5 in relation to CFA5. The gene expression of TGF-β increased in cases that had received folic acid at two and five months, and VEGF decreased in the CFA5 and DFA5 groups. DC5 showed increased VEGF expression in comparison with CC5. Conclusions. Gestational diabetes mellitus and folic acid both change the expression of inflammatory markers, thus demonstrating that the exposure to harmful agents in adulthood has a more severe impact in cases which underwent fetal reprogramming.http://dx.doi.org/10.1155/2016/9151607 |
| spellingShingle | Carlos Donizete Pereira Júnior Camila Souza de Oliveira Guimarães Aline Cristina Souza da Silva Aldo Rogelis Aquiles Rodrigues Maria Aparecida da Glória Vicente de Paula Antunes Teixeira Niels Olsen Saraiva Câmara Lenaldo Branco Rocha Marlene Antônia dos Reis Juliana Reis Machado Laura Penna Rocha Fernanda Rodrigues Helmo Rosana Rosa Miranda Corrêa Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure Journal of Immunology Research |
| title | Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure |
| title_full | Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure |
| title_fullStr | Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure |
| title_full_unstemmed | Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure |
| title_short | Influence of the Expression of Inflammatory Markers on Kidney after Fetal Programming in an Experimental Model of Renal Failure |
| title_sort | influence of the expression of inflammatory markers on kidney after fetal programming in an experimental model of renal failure |
| url | http://dx.doi.org/10.1155/2016/9151607 |
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